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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Schneider, H. Articles by Giebel, L. B. Search for Related Content PubMed PubMed Citation Articles by Schneider, H. Articles by Giebel, L. B. Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, Vol. 89 No. 2 (January 15), 1997: pp. 473-482 Homodimerization of Erythropoietin Receptor by a Bivalent Monoclonal Antibody Triggers Cell Proliferation and Differentiation of Erythroid Precursors By Helmut Schneider, Warak Chaovapong, David J. Matthews, Cyrus Karkaria, Robert T. Cass, Hangjun Zhan, Mark Boyle, Tony Lorenzini, Steve G. Elliott, and Lutz B. Giebel From the Department of Receptor Biology, Arris Pharmaceutical Corp, South San Francisco; and the Amgen Center, Amgen, Thousand Oaks, CA.     ABSTRACT Abstract Introduction Methods Results Discussion References Erythropoietin (EPO) stimulates proliferation and differentiation of erythroid progenitor cells. Several lines of evidence indicate that the most likely mechanism of EPO receptor (EPO-R) activation by EPO is homodimerization of the receptor on the surface of erythrocyte precursors. Therefore, we argued that it should be possible to raise EPO-R monoclonal antibodies (MoAbs) that would activate the receptor by dimerization and thus mimic EPO action. We have identified such an agonist MoAb (MoAb34) directed against the extracellular EPO binding domain of the EPO-R. This bivalent IgG antibody triggers the proliferation of EPO-dependent cell lines and induces differentiation of erythroid precursors in vitro. In contrast, the monovalent Fab fragment, which cannot dimerize the receptor, is completely inactive. The mechanism of receptor activation by homodimerization implies that at high ligand concentrations the formation of 1:1 receptor/ligand complexes is favored over 2:1 complexes, thereby turning the ligand agonist into an antagonist. Thus, EPO and MoAb34 should self-antagonize at high concentrations in both cell proliferation and differentiation assays. Our data indeed demonstrate that EPO and MoAb34 antagonize ligand-dependent cell proliferation with IC50 values of approximately 20 and 2 µmol/L, respectively. Erythroid colony formation (BFUe) is inhibited at MoAb34 concentrations above 1 µmol/L. Furthermore, we analyzed the MoAb34:EPO-R interaction using a mathematic model describing antibody-mediated receptor dimerization. The data for proliferation and differentiation activity were consistent with the receptor dimer formation on the cell surface predicted by the model.     INTRODUCTION Abstract Introduction Methods Results Discussion References ERYTHROPOIETIN (EPO), a 34-kD glycoprotein hormone, is the major regulator of mammalian erythropoiesis.1 EPO acts on erythroid progenitor cells by preventing apoptosis,2,3 stimulating proliferation of erythroid precursor cells, and inducing differentiation into mature erythrocytes. These effects are transduced by the binding of EPO to a specific EPO receptor (EPO-R) on the surface of committed erythroid progenitor cells.4 Deletion of EPO and EPO-R genes in mice has shown that EPO is crucial for the survival, proliferation, and differentiation of late committed progenitors (colony-forming unit-erythroid [CFU-E]), but not of early progenitors (burst-forming unit-erythroid [BFU-E]).5 Mice homozygous for a deletion of either EPO or EPO-R genes die during embryogenesis due to failure of erythropoiesis in the fetal liver. The EPO-R is a member of the cytokine receptor type I superfamily, which includes the receptors for interleukin-2 (IL-2) through IL-7, granulocyte-macrophage colony-stimulating factor, granulocyte colony-stimulating factor (G-CSF ), growth hormone (GH), prolactin (PRL), thrombopoietin (TPO), leukemia inhibitory factor, and leptin.6-8 The receptors for EPO, GH, PRL, TPO, and G-CSF appear to be triggered by ligand-induced receptor homodimerization.4,7,9 For the EPO-R, direct evidence for the dimerization model has been provided by the recent discovery of a dimeric peptide that binds to and activates the receptor.10 The crystal structure of the peptide in complex with the extracellular domain of the EPO-R (EPO binding protein [EPObp]) shows that the peptide dimer binds to two molecules of EPObp.10 The formation of complexes between EPO and two molecules of EPObp in solution has been described using light-scattering, sedimentation equilibrium, and titration calorimetry techniques.11 Stable EPObp2EPO complexes have also been purified (Zhan H, Karkaria C, Koe G, Savel L, Giebel LB, manuscript submitted). Previous evidence for EPO-induced receptor dimerization on the cell surface is based primarily on constitutively active EPO-R mutants, which contain point mutations introducing cysteine substitutions into the extracellular domain at amino acid positions R129, E132, and E133.12-16 The EPO-R mutants form disulfide-linked homodimers in the endoplasmic reticulum and on the cell surface.14 Based on sequence alignments with the related GH receptor, these mutations are expected to be in the receptor-dimer interface region. Expression of the constitutively active EPO-R (R129C) mutant in BaF3 cells results in factor-independent proliferation, and expression in primary cultures of mouse fetal liver cells induces EPO-independent erythroid differentiation.17 Furthermore, mice infected with a retrovirus carrying the EPO-R (R129C) mutant develop erythroleukemia.12 Truncated receptor mutants that lack part of the intracellular signaling domain are dominant-negative for signal transduction when coexpressed with the wild-type EPO-R.15,18 Both wild-type and truncated receptors can be coimmunoprecipitated with an antibody directed against the C-terminus of the wild-type receptor, which is not present in the truncated form,19 further suggesting the presence of receptor dimers on the cell surface. Receptor dimerization has been analyzed in great detail for the GH receptor.20,21 GH has two distinct receptor binding sites. At high ligand concentrations, formation of 1:1 complexes via the high-affinity GH site 1 is favored over 2:1 complexes, preventing GH receptor signaling, and Fuh et al22 demonstrated self-antagonism of GH in a GH-dependent cell proliferation assay at GH concentrations greater than 100 nmol/L. Homodimerization and signal transduction of GH receptor can also be achieved by specific monoclonal receptor antibodies. IgG-type antibodies are bivalent molecules that bind to two antigen molecules at the same time. IgGs specific to GH receptor therefore are able to dimerize and stimulate the receptor. Like GH, these antibodies are self-antagonists at high concentrations.22 Similar agonist antibodies have also been described for the PRL receptor.23 We have raised a monoclonal antibody (MoAb) directed against EPObp, which mimics EPO action by inducing ligand-dependent cell proliferation and differentiation. Self-antagonism of EPO has not been reported so far, probably because the concentrations of EPO tested have not been high enough. We analyzed EPO at concentrations up to 30 µmol/L and were able to detect specific inhibition of EPO-dependent proliferation. This provides further evidence that EPO triggers its receptor by ligand-induced dimerization.     MATERIALS AND METHODS Abstract Introduction Methods Results Discussion References Cell lines. BaF3, a murine IL-3-dependent cell line,24 was a gift from Dr H. Lodish (Whitehead Institute, Cambridge, MA). An EPO-dependent BaF3/EPO-R cell line was generated by transfecting the full-length human EPO-R into BaF3 cells. A cDNA encoding the full-length human EPO-R (a gift from Dr J. Winkelmann, University of Cincinnati, OH; nucleotide sequence identical to that used by Winkelmann et al25 and Jones et al26 ) was cloned into plasmid expression vector pRc/CMV (Invitrogen, San Diego, CA). After electroporation into BaF3 cells, the cells were cultured for 2 days in RPMI 1640 medium containing 10% fetal bovine serum (FBS) and IL-3. Cells were washed twice, transferred into RPMI 1640 medium plus 135 pmol/L EPO, and selected for EPO-dependent growth. Individual clones were selected by limiting-dilution cloning. The EPO-dependent cell line chosen for this study proliferates in the presence of EPO with an EC50 of 15 pmol/L. Scatchard analysis showed 800 receptors per cell that bind EPO with 300-pmol/L affinity if, for simplification, single-site binding is assumed (data not shown). Cells were maintained in RPMI 1640 medium with 10% FBS, 20 mmol/L HEPES, pH 7.8, and 10 µmol/L mercaptoethanol supplemented with 100 pmol/L EPO. BaF3 cells were supplemented with 10% IL-3 containing WEHI-3B-conditioned medium. UT-7/EPO cells27 were a gift from Dr Norio Komatsu, and were grown in 1X Iscove's modified Dulbecco's medium (IMDM) with L-glutamine, 25 mmol/L HEPES, 3.024 g/L sodium bicarbonate, 10% FBS, and 1% L-glutamine-penicillin-streptomycin solution (Irvine Scientific, Santa Ana, CA) containing 270 pmol/L EPO. Expression and affinity purification of soluble human EPO-R. DNA encoding a soluble truncated EPO-R (EPObp) was generated by polymerase chain reaction (PCR) using the full-length cDNA as template. The amplification product introduces a TAG termination codon 5' of the transmembrane region and encodes the extracellular domain consisting of amino acids 1 through 249 of the published sequence.26 The PCR product was subcloned into expression vector pRc/CMV (Invitrogen) and stably transfected into CHO cells. Individual clones secreting EPObp were selected by limiting-dilution cloning. Roller bottles (surface area, 1,700 cm2; Corning, Corning, NY) were seeded with the stable cell line, which was then grown to confluence in RPMI 1640 medium plus 10% FBS. The cells were washed twice in serum-free RPMI 1640 medium and cultured in 200 mL serum-free RPMI 1640 medium. Cell supernatant was collected after 2 days, and fresh medium was added for another 2 days. EPO was oxidized with 10 mmol/L NaIO4 and biotinylated using 10 mmol/L biotin hydrazide (Pierce, Rockford, IL) following the manufacturer's instructions. A ligand affinity column was prepared by immobilizing biotinylated EPO (10 mg) on Streptavidin 3M Emphaze beads (3 mL; Pierce) overnight in Dulbecco's phosphate-buffered saline ([PBS] Irvine Scientific) at 4°C. The beads were separated from the supernatant by centrifugation, and incubated with 10 mmol/L biotin in PBS for 2 hours at 4°C to saturate all biotin binding sites. After washing with PBS, the EPO-coated beads were packed in a glass column (Omnifit; Alltech Corp, Deerfield, IL). Cell supernatant (10 L) was concentrated and diafiltered to 1 L in 20 mmol/L Tris hydrochloride, pH 7.6, and loaded on the column at a flow rate of 0.7 mL/min. The column was washed with 50 mL 20-mmol/L Tris hydrochloride, pH 7.6. Bound EPObp was eluted with 750 mmol/L NaCl in 20 mmol/L Tris hydrochloride, pH 7.6. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed a single 30-kD EPObp band. The EPObp fractions were pooled and concentrated, and the buffer was exchanged with PBS to a final concentration of 0.8 mg/mL. Generation and screening of MoAb. Five balb/c mice were immunized by seven subcutaneous injections at two sites over a period of 14 weeks. Each 50-µL injection contained 25 µg EPObp in Freund's adjuvant. After 12 weeks, all mice had developed anti-EPObp antibody titers. The dilutions needed to reach a half-maximal signal in an enzyme-linked immunosorbent assay (ELISA) ranging from 1:20,000 to 1:50,000. An additional final injection of 150 µg EPObp was administered intravenously to the mouse expressing the highest antibody titer. After 3 days, spleen cells were isolated and fused with myeloma strain P3X63Ag8.653 (American Type Culture Collection, Rockville, MD; CRL 1580). After selection in hypoxanthine-aminopterin-thymidine medium (Sigma, St Louis, MO) for 10 days, a total of 475 supernatants were screened for specific antibody production by ELISA. Positive clones were transferred to 24-well microtiter plates, and supernatants were assayed in a thymidine uptake proliferation assay using the cell line BaF3/EPO-R and the parental BaF3 as a control. Hybridoma clone MoAb34 was subcloned twice by limiting dilution. Ig isotyping was performed using the IsoStrip Mouse Monoclonal Antibody Isotyping Kit from Boehringer Mannheim (Indianapolis, IN). Purification of MoAb34 and Fab preparation. Hybridomas were grown in 47.5% RPMI 1640 medium, 47.5% Dulbecco's modified Eagle's medium, and 5% FBS. Culture supernatant was filtered through a 0.2-µm membrane. A 6-mL protein G-Sepharose 4 fast-flow column (Pharmacia Biotech, Piscataway, NJ) was packed with 80 psi pressure. A 1-L sample was loaded at 4 mL/min at 4°C, followed by washing with greater than 5 column vol PBS. MoAb34 was then eluted from the column with ImmunoPure IgG elution buffer (Pierce) at 2 mL/min. The eluate was immediately neutralized to pH 7.5 by adding 3 mol/L Tris. The purity was evaluated by nonreducing SDS-PAGE. Fab fragments were generated by papain cleavage using the ImmunoPure IgG1 Fab Preparation Kit (Pierce) following the manufacturer's instructions. The Fab was further purified by gel filtration using a Superdex 75 column (1.6 cm × 60 cm; Pharmacia), eluted with PBS, and analyzed for purity by SDS-PAGE. ELISA. Because we expected the number of agonist antibodies to be low, we used two different methods for immobilization of the EPObp to ensure identification of a maximum number of anti-EPObp MoAbs. In ELISA 1, EPObp was covalently immobilized. EPObp was oxidized in 1 mmol/L NaIO4 and 50 mmol/L sodium acetate, pH 5.5, at 4°C for 30 minutes in the dark. The protein was separated from periodate on a NAP-5 column (Pharmacia) and incubated on hydrazide-activated microtiter plates (Unisyn, San Diego, CA) at 2 µg/mL (100 µL per well) for 1 hour at room temperature. Plates were washed and blocked with PBS and 20 mg/mL bovine serum albumin (BSA) for 1 hour. In ELISA 2, Polysorb microtiter plates (Nunc, Roskilde, Denmark) were incubated for 1 hour at 37°C with 10 µg/mL MoAb 2E12, a specific, nonneutralizing rat MoAb directed against EPObp (B. van Dyke, personal communication, February 1994). The plates were washed and blocked, and then EPObp (1 µg/mL) was added in ELISA buffer (PBS, 1 mg/mL BSA, and 0.02% Tween-20) for 1 hour at 37°C. After immobilization of EPObp, both ELISA protocols were identical. Antibody-containing samples in ELISA buffer were added and incubated for 1 hour at 37°C. After washing, plates were incubated with a sheep anti-mouse IgG coupled to horseradish peroxidase ([HRP] Sigma) at 0.1 ng/mL in ELISA buffer for 1 hour at 37°C. The plates were then washed, and 100 µL TMB/H2O2 developing solution (Pierce) was added and incubated for 5 minutes. Color development was stopped by adding 100 µL 1-mol/L sulfuric acid, and the OD450-650 was determined using a plate reader (Molecular Devices, Sunnyvale, CA). Thymidine uptake proliferation assays. BaF3 and BaF3/EPO-R cells were grown to the late logarithmic phase, collected by centrifugation, washed three times with RPMI 1640 media containing 10% FBS and 10 mmol/L HEPES, pH 7, in the absence of EPO and IL-3, and then starved in the same media for 2 hours. Antibody test samples (hybridoma supernatants or purified proteins) were diluted at least fourfold into 100 µL media, and 100 µL cells were added (25,000 cells per well). EPO was dialyzed against 10 mmol/L HEPES, pH 7.0, and 100-µL test samples were combined with 100 µL cells (25,000 cells per well) in twofold-concentrated medium. Plates were incubated for 4 hours at 37°C and 5% CO2 in a humidified tissue culture incubator. Then, 0.5 µCi methyl-[3H]thymidine (Amersham, Arlington Heights, IL; 1 mCi/mL, 20 Ci/mmol) diluted into 20 µL medium, was added, and the incubation was continued for another 15 hours. Cells were harvested onto glass fiber filtermats using a Tomtec cell harvester, and incorporated radiolabel was determined using a Microbeta 1450 scintillation counter (Wallac, Turku, Finland). UT-7/EPO cells were grown to approximately 3 × 105/mL, collected by centrifugation, washed twice with PBS, and resuspended at 50,000/mL in assay medium (RPMI 1640 medium with 1% L-glutamine and 4% FBS). Tissue culture plates (96-well) were loaded with 100-µL test samples (diluted at least fivefold in assay medium) and 50 µL cells per well and incubated at 37°C and 5% CO2 . After 72 hours, 0.5 µCi methyl-[3H]thymidine diluted in 50 µL assay medium was added, and the cells were incubated for another 4 hours at 37°C and 5% CO2 . Labeled cells were harvested onto glass fiber filtermats using a PHD cell harvester (Cambridge Technology, Inc, Watertown, MA). Filters were rinsed with 2-propanol, dried, and counted in a Beckman model LS6000IC scintillation counter (Fullerton, CA). Cell-based EPO binding-competition assay. OCIM1 cells, a human erythroleukemia cell line that expresses EPO-R on the cell surface,28 were grown in IMDM, 10% FBS, and 1% penicillin-streptomycin to approximately 2 to 5 × 105 cells/mL. Cells were collected by centrifugation, washed two times in binding buffer (RPMI 1640 medium, 1% BSA, and 25 mmol/L HEPES, pH 7.3), and resuspended in binding buffer containing 0.1% NaN3 and 10 µg/mL cytochalisin B at 1 to 2 × 107 cells/mL. Tissue culture plates (96-well) were loaded with 100 µL cells, 10 µL sample, and 10 µL [125I]-EPO (Amersham, high specific activity, 3,000 Ci/mmol, 2 mCi/mL) and incubated for 3 hours at 37°C in a humidified tissue culture incubator. Then, the cells were centrifuged through phthalate oil (60:40 vol/vol dibutyl/dinonyl phthalate) in titer tubes. The tubes containing cell pellets were quick-frozen in a dry ice-ethanol bath, and the cell pellet was clipped and then counted in a Pharmacia-LKB (Uppsala, Sweden) 1277 Gammamaster automatic gamma counter. BFUe cell differentiation assay. Normal human donors underwent lymphopheresis according to standard protocols to purify CD34+ erythroid cells.29 Human blood was obtained after informed consent. The cells were washed, resuspended in Hanks balanced salt solution (HBSS), and separated by density centrifugation over a gradient (Ficoll-Paque; Pharmacia Biotech). The low-density cells were collected, washed with HBSS, and resuspended in PBS supplemented with 0.5% BSA and 5 mmol/L EDTA at a concentration of 5 × 108 cells/mL. From these cells, purified CD34+ cells were obtained using a CD34 Progenitor Cell Isolation Kit (QBend/10; Miltenyl Biotech GmbH, Bergisch Gladbach, Germany). The in vitro BFU-E assay on purified CD34+ cells was performed in methylcellulose. The medium contained 20% FBS, 0.33X IMDM (GIBCO, Grand Island, NY), salts, 2-mercaptoethanol, nucleosides, cholesterol, sodium pyruvate, Hu-transferrin, lipids, Hu-insulin, deionized BSA, and 100 ng/mL stem cell factor (SCF ).30 A suspension of CD34+ cells (10,000/mL), 0.015 mL SCF (20 µg/mL), and a combination of sample and medium totaling 3 mL was prepared in sterile polystyrene tubes. Duplicate 1-mL aliquots were placed onto 35 × 100-mm tissue culture plates. The plates were incubated at 37°C and 10% CO2 in a humidified tissue culture incubator. Erythroid colonies (orange to red in color) were scored after 19 to 20 days. BIAcore analysis. Kinetic parameters for the interaction of MoAb34 and its Fab fragment (Fab34) with EPObp were measured using real-time biospecific interaction analysis (BIAcore).31 The BIAcore system, CM-5 sensor chip, and reagents were from Pharmacia Biosensor (Piscataway, NJ). All injections on the sensor chip surface were at a flow rate of 5 mL/min and 25°C unless otherwise stated. Between injections of reagents, the sensor chip was continuously washed with 10 mmol/L HEPES, pH 7.2, 150 mmol/L NaCl, 3.4 mmol/L EDTA, and 0.005% surfactant P20 . The interaction of MoAb34 with EPObp was characterized by coupling approximately 6,800 resonance units (RU) of MoAb34 to the sensor chip surface using standard amine immobilization chemistry.32 EPObp samples of 10 to 1,500 nmol/L were injected for 7 minutes over the MoAb34 surface and over a control flow cell. After each injection of EPObp, a 1-minute pulse of 1 mmol/L formic acid was used to regenerate the MoAb34 surface. To measure the interaction of EPObp and Fab34, oxidized EPObp (~500 RU) was immobilized via carbohydrazide coupling to the carboxymethylated dextran matrix.33 Injections of Fab34 spanned a concentration range of 1 to 500 nmol/L. After each injection of Fab34, the EPObp surface was regenerated with a 50-µL pulse, at 50 µL/min, of 10 mmol/L 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS; pH 10.4). Association and dissociation rate constants were determined by least-squares fitting using BIAevaluation software (Pharmacia Biosensor). To minimize potential rebinding effects, only the initial 15 seconds of each dissociation profile was used for calculation of the dissociation rate constant. View larger version (14K): [in this window] [in a new window]   Fig 1. MoAb34 immunoblot analysis of EPObp. EPObp was heat-denatured and analyzed by reducing SDS-PAGE on a 12% acrylamide gel and subsequent transfer to nitrocellulose. The blot was incubated with MoAb34 (10 µg/mL) and subsequently with antimouse IgG coupled to horseradish peroxidase. View larger version (23K): [in this window] [in a new window]   Fig 2. BIAcore analysis of MoAb34. Sensorgrams of various concentrations of EPObp (10 to 1,500 nmol/L) injected over immobilized MoAb34, corrected by subtraction of data from control surfaces. The ligand was removed after 420 seconds, and dissociation was measured after this point. Data from 8 representative free-EPObp concentrations (nmol/L) from a total of 16 are shown: 1,500 (a), 750 (b), 150 (c), 100 (d), 75 (e), 50 (f ), 25 (g), and 10 (h).     RESULTS Abstract Introduction Methods Results Discussion References MoAb34 stimulates proliferation of BaF3/EPO-R cells. We have isolated a total of 48 MoAbs specific for EPObp, the soluble extracellular ligand-binding domain of the human EPO-R. The hybridoma supernatant of MoAb clone 34 stimulated thymidine uptake in BaF3/EPO-R cells, but did not stimulate proliferation in parental BaF3 cells. MoAb34 is an IgG MoAb that was subtyped as IgG1 . Immunoblot analysis of heat-denatured and reduced EPObp suggested that MoAb34 recognizes a linear epitope (Fig 1). MoAb34 did not compete with [125I]-EPO in a binding-competition assay using human OCIM1 cells28 (data not shown). Thus, MoAb34 does not interfere with the binding of EPO to its receptor, indicating that there is no overlap of MoAb34 and EPO binding epitopes on the receptor. The binding kinetics of both MoAb34 and Fab34 to EPObp were characterized using surface plasmon resonance (Fig 2). For MoAb34 kinetics, the antibody was immobilized to avoid avidity effects. For Fab34 kinetics, we immobilized EPObp. The kinetic constants for MoAb34 and Fab34 are summarized in Table 1.   View this table: [in this window] [in a new window]   Table 1. BIAcore Analysis of MoAb34 and Fab34 Interactions With EPObp MoAb34, but not the Fab fragments, stimulate proliferation in EPO-dependent cells. Purified MoAb34 was able to stimulate proliferation in EPO-dependent cell lines. A dose-dependent response evaluation in a [3H]thymidine uptake cell proliferation assay showed EC50 values of approximately 10 nmol/L (Fig 3A) in BaF3/EPO-R cells. The effect of MoAb34 was specific to EPO-R, because it did not stimulate growth of the parental BaF3 cell line (data not shown). Under identical conditions, the maximal amount of [3H]thymidine incorporation caused by EPO (Fig 4) was eightfold to 10-fold higher than the incorporation caused by MoAb34 (Fig 3A). In contrast to the bivalent MoAb34, monovalent Fab fragments did not stimulate proliferation of the BaF3/EPO-R cell line (Fig 3A), even though the affinities of MoAb34 and Fab34 to EPObp were similar (Table 1). MoAb34 was even more active in the cell line UT-7/EPO,27 which expresses endogenous EPO-R, where it stimulated proliferation with an EC50 of approximately 300 pmol/L (Fig 3B). The maximum incorporation was close to the value obtained with EPO. This may be due to the higher concentration of EPO-R molecules on the surface of UT-7/EPO cells, which contain 2,400 receptors per cell,27,34 versus 800 for BaF3/EPO-R (data not shown). For higher receptor concentrations, the ligand concentration necessary to induce dimerization of the receptors should be lower. At higher concentrations (>200 nmol/L), MoAb34 antagonizes cell proliferation in both cell lines (Fig 3), as expected based on the homodimerization model. The resulting dose-dependent proliferation curves have a bell-shaped character with IC50 values for self-antagonism of approximately 2 µmol/L. View larger version (17K): [in this window] [in a new window]   Fig 3. Dose-dependent proliferation of EPO-sensitive cells by MoAb34. (A) Proliferation of BaF3/EPO-R cells in the presence of [3H]thymidine and various concentrations of MoAb34 () and Fab34 (). (B) Proliferation of UT-7/EPO in the presence of [3H]thymidine and various concentrations of MoAb34 (), control (anti-Axl) antibody (), and EPO (), respectively. Experiments were made in duplicate; the mean values are shown. View larger version (17K): [in this window] [in a new window]   Fig 4. Effect of high EPO concentrations on the proliferation of BaF3/EPO-R cells () and parental BaF3 cells (). Both cell lines were assayed with identical dilution series of EPO. BaF3 cells were supplemented with 10% WEHI-conditioned media as a source for IL-3. Experiments were made in duplicate; the mean values are shown. If EPO homodimerizes the receptor, then self-antagonism should also be observed at high EPO concentrations. We demonstrated this using our BaF3/EPO-R cell proliferation assay (Fig 4). Proliferation significantly decreased above 3 µmol/L; however, complete inhibition was not observed at the concentrations tested. The estimated IC50 value was approximately 20 µmol/L, representing 74,000 U/mL or 370 µg/mL of EPO. This is an extremely high concentration BaF3/EPO-R cells proliferate with an EC50 of 15 pmol/L, which is six orders of magnitude lower. To confirm that the decrease in signal in BaF3/EPO-R cells was specific to EPO and not due to toxicity or other artifacts at such high ligand concentrations, parental BaF3 cells were incubated with EPO at identical concentrations in the presence of IL-3. No decrease in IL-3-dependent proliferation was observed at any EPO concentration (Fig 4). MoAb34 induces differentiation of CD34+ erythroid progenitor cells in the presence of SCF. The differentiation of BFUe cells in the CD34+ population to erythroid bursts is dependent on EPO and SCF. MoAb34 was able to induce in vitro differentiation of human CD34+ erythroid cell precursors. In two independent experiments, duplicate sets of CD34+ cells were treated with various concentrations of MoAb34, EPO, or a control antibody. The cells were incubated in the presence of a fixed concentration of SCF (100 ng/mL). After 19 days in the first experiment and 20 days in the second experiment, colonies from BFUe cells were visible in the presence of either MoAb34 or EPO, but not in the antibody control (Table 2). The colonies showed typical red color and could be identified as erythroid cells by microscopic analysis (Fig 5). As in the cell proliferation assays described earlier, EPO was more potent than MoAb34. Colonies developed at the lowest EPO concentration tested (1.3 pmol/L), whereas no erythroid colonies were observed at a MoAb34 concentration less than 7 nmol/L. Both the absolute number of colonies and the size of the colonies were higher in the presence of EPO than in the presence of MoAb34. The approximate EC50 value was 15 nmol/L, and the highest stimulation of differentiation by MoAb34 observed was 22 to 220 nmol/L, similar to the maxima observed in the cell proliferation assays (Table 2). In addition, at concentrations above 720 nmol/L, MoAb34 self-antagonizes in this cell differentiation assay. All these data demonstrate that both cell proliferation and differentiation are driven by ligand-induced receptor homodimerization.   View this table: [in this window] [in a new window]   Table 2. BFUe In Vitro Differentiation Assay View larger version (117K): [in this window] [in a new window]   Fig 5. BFUe colonies stimulated by MoAb34 and EPO. Purified CD34+ cells from human peripheral blood were incubated for 20 days. BFUe colonies were identified by microscopic examination and photographed. Representative colonies obtained at 2 different concentrations of EPO and MoAb34, respectively, are shown. (A) 144 nmol/L MoAb34; (B) 36 nmol/L MoAb34; (C) 130 pmol/L EPO; (D) 6.6 pmol/L EPO. Agonist activity of MoAb34 correlates well with a model for antibody-mediated receptor dimerization. Mathematic models have been developed to describe the formation of receptor dimers on the cell surface by bivalent ligand antibodies35 or by GH.36 We investigated how the agonist activity of MoAb34 would correlate with the occurrence of receptor dimers predicted by the model of Perelson.35 Briefly, Perelson postulates a two-step mechanism, whereby the formation of 1:1 complexes is driven by the affinity constant KA (= 1 KD ). Subsequent dimer formation is dependent on a "cross-linking" constant KX , which includes KA but also depends on the effective concentration of receptors on the cell surface and other factors. In the following equations, "Ab" stands for antireceptor antibody and "R" for receptor: The concentration of dimer is calculated as assuming that the amount of antibody bound is small compared with the total antibody concentration. The maximal concentration of dimer is solely dependent on KA : If the percentage of receptor/antibody 2:1 complexes versus the total number of receptors is plotted against the antibody concentration, a symmetric bell-shaped curve is predicted. The maximum of 2:1 complexes occurs at a defined antibody concentration equal to half the antibody Kd value. Details of the above derivation may be found elsewhere.35 View larger version (17K): [in this window] [in a new window]   Fig 6. Fit of data obtained from in vitro cell assays to a mathematic model describing antibody-mediated receptor dimerization. Determination of KD by BIAcore analysis showed a value of ~84 nmol/L. Baseline values for proliferation ([3H]thymidine incorporation in the absence of MoAb34) were subtracted. (A) Proliferation of BaF3/EPO-R cells; (B) proliferation of UT-7/EPO cells; (C) differentiation of CD34+ cells. The mean number of colonies obtained in the 2 counts of experiment 2 (Table 2) was used. Figure 6 fits the data of the MoAb34 cell proliferation and differentiation assays to the equation. The resulting bell-shaped curves for the proliferation assays correlate well with the assay data. The obtained 2:1 complex maxima were 114 nmol/L for BaF3/EPO-R cells and 26 nmol/L for UT-7/EPO cells. According to the model, this translates to apparent Kd values of 228 and 52 nmol/L, respectively, in good agreement with the Kd value of 84 nmol/L determined by BIAcore analysis (Table 1). These results demonstrate that the agonist activity of the bivalent MoAb34 in cell proliferation and differentiation assays is consistent with ligand-induced homodimerization of the EPO-R on the cell surface.     DISCUSSION Abstract Introduction Methods Results Discussion References Homodimerization of the EPO-R by EPO on the cell surface is believed to be the key event in signal transduction.4 The model for homodimerization of the EPO-R by EPO implies that it should be possible to trigger the receptor by bivalent MoAbs directed against EPObp. For the related GH receptor, a variety of agonist MoAbs have been reported.22 MoAb34 is such a bivalent IgG with the ability to dimerize two receptor molecules. We have shown stimulation of cell proliferation in BaF3/EPO-R and UT-7/EPO, as well as stimulation of cell differentiation in CD34+. On the other hand, monovalent Fab fragments, which cannot form receptor dimers, are totally unable to stimulate cell proliferation in BaF3/EPO-R, although their affinity for EPObp is similar. In all three test systems used, we observed self-antagonism of MoAb34 at high concentrations. This is precisely what the homodimerization mechanism requires: when high ligand concentrations drive the equilibrium from 2:1 complexes toward 1:1 complexes, there are fewer receptor dimers on the cell surface triggering signal transduction. Self-antagonism has been described for agonist GH receptor and PRL receptor antibodies, too,22,23 suggesting that all three receptors are activated by the same principle. Over the full antibody concentration range, activation of cell proliferation and differentiation shows a bell-shaped response curve. The maximum observed in all three in vitro experiments occurs at similar MoAb34 concentrations, in close vicinity to its Kd . Indeed, a mathematic model35 predicts that a maximum of 2:1 receptor/antibody complexes is formed at a concentration of 0.5 × Kd . We were able to fit the data obtained in the cell proliferation and differentiation assays to this model. The correlation of agonist activity with the predicted occurrence of 2:1 complexes further supports the homodimerization model. In contrast, data reported for agonist antibodies stimulating the GH and PRL receptor show a maximum response in proliferation assays at concentrations approximately two orders of magnitude higher than their Kd values.22,23 It is possible that the affinities of anti-PRL receptor antibodies were overestimated due to avidity effects, since they were determined by binding analysis of radiolabeled antibodies to whole-cell homogenates.37 In the GH-GH receptor complex, there is a substantial contact surface between the two GH receptor molecules in addition to the hormone-receptor interfaces, which contributes to the binding energy.21 In contrast, receptor-receptor interaction in the EPObp2EPO complex seems to be poor, if there is any.11 We have demonstrated for the first time that EPO exhibits self-antagonism (Fig 4). The concentration of EPO needed was more than four orders of magnitude higher than that necessary for a maximal response. The likely reason that this effect has not been reported previously is that the micromolar concentrations necessary have not been tested in proliferation assays. The IC50 for EPO self-antagonism is approximately 10-fold higher than the IC50 for human GH self-antagonism, whereas EC50 values for agonism of EPO and GH are similar.22 There are several explanations possible. The IC50 of EPO self-antagonism could be higher due to a lower affinity of site 1 for EPO than for GH. Alternatively, different receptor densities and site 2 affinities could account for the observed discrepancies. Unfortunately, the available data are limited. A well-derived Kd is available for GH (Kd = 0.3 nmol/L),22 but the corresponding site of EPO has not been as well characterized (Kd , ~0.5 nmol/L).11 On the other hand, the Kd of site 2 has been well determined for EPO (0.85 to 1.35 µmol/L),11 but it is unknown for GH. Previous study has demonstrated that there are interactions between the extracellular domains of GH receptor21; however, the contributions of the membrane-spanning and intracellular domains to the dimerization of cytokine receptors are poorly understood. A counterpart to the inactive Fab fragments of MoAb34 would be an EPO mutant that lacks the putative second binding site. Such a mutant would still be able to bind to the receptor, but it would not be able to cause dimerization and therefore should be inactive in a proliferation assay. For GH, Fuh et al22 have shown that a mutant in which residue Gly-120 is replaced by arginine disrupts the site 2 receptor binding site. This mutant binds to GH receptor with the same affinity as the wild-type hormone, but it cannot stimulate the receptor and it acts as an antagonist. Such an EPO mutant has been described recently38 and gives further evidence that EPO acts through homodimerization of its receptor. Why are agonist antibodies for EPO-R so rare? All MoAbs specific to the extracellular domain should dimerize the receptor because they are bivalent. However, the vast majority of antibodies in our screen are not agonists (47 of 48) and form inactive 2:1 complexes. Although generation of specific MoAbs with antagonist activity has been reported previously,39-41 agonist antibodies have not been described. Apparently, the cell surface imposes steric constraints and the two receptor subunits in the 2:1 complex have to be at a specific orientation and/or distance relative to each other. If this is not the case, receptor-receptor interactions necessary for signal transduction cannot be formed. Stimulation of EPO induces binding of a JAK2 kinase molecule to the cytosolic domain of each EPO-R molecule and increases phosphorylation of EPO-R and JAK2 kinase itself.42 If the latter is an intermolecular phosphorylation process as has been described for receptor tyrosine kinases,43,44 close proximity of the two receptor molecules would be essential. Because of the size of the antibody, this proximity is unlikely in most antibody-receptor complexes. Since MoAb34 is a less potent agonist than EPO, it suggests that it dimerizes EPO-R in a slightly different way than the natural hormone does, and that this dimerization is suboptimal for signal transduction.     FOOTNOTES    Submitted May 1, 1996; accepted September 3, 1996.    Address reprint requests to Helmut Schneider, PhD, Arris Pharmaceutical Corp, 385 Oyster Point Blvd, South San Francisco, CA 94080.    The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hearly marked ``advertisment'' in accordance with 18 U.S.C. section 1734 solely to indicate this fact.     ACKNOWLEDGMENT We thank Dr H. Lodish for providing the BaF3 cell line, Dr J. Winkelmann for human EPO-R plasmid pLAP-37, Dr N. Komatsu for the cell line UT-7/EPO, Dr T. Papayannopoulou for OCIM1 cells, and B. van Dyke for the antibody 2E12. We are grateful to Drs H. Lodish and D. Milligan for stimulating discussion.     REFERENCES Abstract Introduction Methods Results Discussion References 1. Krantz SB: Erythropoietin. Blood 77:419, 1991[Free Full Text] 2. Koury MJ, Bondurant MC: Erythropoietin retards DNA breakdown and prevents programmed death in erythroid progenitor cells. Science 248:378, 1990[Abstract/Free Full Text] 3. Zhuang H, Niu Z, He TC, Patel SV, Wojchowski DM: Erythropoietin-dependent inhibition of apoptosis is supported by carboxyl-truncated receptor forms and blocked by dominant-negative forms of Jak2. 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