Blood, 1 June 1999, Vol. 93, No. 11, pp. 4030-4030
ERRATUM
ERRATUM
In the article by Shah et al, entitled "Development of a Model for
Evaluating the Interaction Between Human Pre-B Acute Lymphoblastic Leukemic Cells in the Bone Marrow Stromal Cell Microenvironment," which appeared in the November 15, 1998 issue (Vol 92, No 10, pp
3817-3828) a correction should be noted regarding the primers used to
amplify p16ink4a locus genes. Figure 10 showed a PCR analysis of the
p16ink4a locus, but the primer pair used to amplify p16 exon 1
was
actually a sequence within the p19 (now designated p14ARF)
exon 1
. Thus, the figure inadvertently shows the PCR results of p19
exon 1 twice. We have subsequently designed correct primers for
amplifying p16 exon 1. The sequence and location of these primers
and probes are as follows:
5' primer: (position 215)
5'-GCC-CAA-CGC-ACC-GAA-TAG-T-3'
3' primer: (position 280)
5'-GCT-CCC-GCT-GCA-GAC-CCT-CTA-3'
probe: (position 239)
5'-TCA-GAG-GCC-GAT-CCA-GGT-G-3'
When this experiment was repeated
with the correct primers, p16 exon 1 was shown to be deleted on both
alleles of BLIN-2. Therefore, the conclusions of the paper are not changed.
