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HEMATOPOIESIS
From the Institute of Hematology, Erasmus University
Rotterdam, Rotterdam, The Netherlands; Department of Molecular Biology,
Jichi Medical School, Kawachi-gun, Tochigi, Japan.
Stem cell factor (SCF) has an important role in the proliferation,
differentiation, survival, and migration of hematopoietic cells. SCF
exerts its effects by binding to cKit, a receptor with intrinsic
tyrosine kinase activity. Activation of phosphatidylinositol 3'-kinase
(PI3-K) by cKit was previously shown to contribute to many SCF-induced
cellular responses. Therefore, PI3-K-dependent signaling pathways
activated by SCF were investigated. The PI3-K-dependent activation and
phosphorylation of the tyrosine kinase Tec and the adapter molecule
p62Dok-1 are reported. The study shows that Tec and Dok-1 form a stable
complex with Lyn and 2 unidentified phosphoproteins of 56 and 140 kd.
Both the Tec homology and the SH2 domain of Tec were identified as
being required for the interaction with Dok-1, whereas 2 domains in
Dok-1 appeared to mediate the association with Tec. In addition, Tec
and Lyn were shown to phosphorylate Dok-1, whereas phosphorylated Dok-1
was demonstrated to bind to the SH2 domains of several signaling
molecules activated by SCF, including Abl, CrkL, SHIP, and PLC Stem cell factor (SCF; also called steel factor,
mast cell growth factor, or Kit ligand) is an important growth factor
for multiple cell types, including hematopoietic progenitor cells. SCF
exerts its effects by binding to the product of the cKit
proto-oncogene.1 cKit is a receptor with intrinsic
tyrosine kinase activity, structurally related to Flt3 and the
receptors for colony-stimulating factor-1 and platelet-derived growth
factor.2 Loss-of-function mutations in the loci for murine
cKit, White Spotting (W), or SCF,
Steel,3-6 lead to macrocytic anemia and to mast
cell deficiency, as well as to a series of nonhematological
defects.1,7,8 Conversely, mutations that render cKit
constitutively active have been found in mastocytoma and
myeloproliferative disease.9,10
Within the hematopoietic compartment, SCF can support the survival and
renewal of the earliest multilineage progenitors and regulates
proliferation and differentiation of mast cell precursors. In
combination with lineage-restricted cytokines, SCF delays
differentiation and enhances the expansion of already committed
progenitors of all lineages.1 This phenomenon is
particularly apparent in erythropoiesis in which the cooperation of
cKit with the erythropoietin receptor (EpoR) is crucial for the
proliferation of erythroblasts.11,12 It has been shown
that cKit and EpoR are closely associated in erythroid
cells,13 but the molecular basis for the synergy between cKit and EpoR or other cytokine receptors has not been resolved.
The profound effects of SCF on proliferation and survival of different
progenitor cell types has raised considerable interest in the
identification of critical signal transduction pathways activated by
cKit. SCF binding to cKit results in dimerization/oligomerization and
subsequent transient tyrosine phosphorylation of the
receptor.14 As a result, proteins of the p21Ras-MAPK
pathway, the phosphatidylinositol 3'-kinase (PI3-K), the tyrosine
kinases Src/Fyn/Lyn and Tec, phospholipase C In vitro studies have shown that activation of PI3-K contributes to
survival, mitogenesis, chemokinesis, and differentiation induced by
SCF.16,24-26 Introduction of cKit mutants unable to activate PI3-K into cKit-deficient mast cells does not restore SCF-induced cell adhesion and only partially restores SCF-induced proliferation.16 Furthermore, we recently observed that
the PI3-K inhibitor LY294002 suppressed the biological effect of SCF on
erythroid progenitors (manuscript in preparation). PI3-K yields PIP3 in
the cell membrane, thereby creating binding sites for pleckstrin
homology (PH) domain containing signaling molecules.27 The
tyrosine kinase Tec is one such PH domain that contains protein activated by cKit.18 Tec is also the founding member of a
family of tyrosine kinases that includes Bruton tyrosine kinase (BTK), Bmx, Itk/Tsk/Emt, and Rlk/Txk.28,29 These kinases are
characterized by a PH and a Tec homology (TH) domain in their
amino-terminus, followed by SH3, SH2, and kinase
domains.30 In contrast to Src kinases, they are devoid of
a membrane-targeting myristylation site, and yet they are rapidly
recruited to the plasma membrane after stimulation of various cytokine
receptors, the T- and B-cell receptors, and CD28.31-35 The
interaction between PIP3 and the PH domain of BTK is critical for this
translocation.36 In the plasma membrane, Tec members are
thought to be activated by Lyn or other members of the Src
family.37,38 Other studies have shown that the TH domain
of Tec is not only essential for the (in)direct interaction with cKit
but also for its association with Lyn and Vav.33,39
In this study, we show that Tec is activated by SCF in erythroid and
megakaryocytic cell lines and that, when activated, it forms a stable
complex with various proteins, including the recently cloned docking
protein p62Dok-1. We found that both the activation of Tec and the
phosphorylation of Dok-1 require PI3-K activity. We show that the TH
and SH2 domains of Tec mediate the interaction with Dok-1, whereas 2 separate domains in Dok-1 are involved in the interaction with Tec. We
further show that Tec, and also Lyn, can phosphorylate Dok-1. Dok-1
contains 15 tyrosine residues, suggesting an important role for Dok-1
in recruiting SH2 domain-containing proteins to the cKit-signaling
complex. In support of this hypothesis, we provide evidence that
phosphorylated Dok-1 binds the SH2 domains of multiple signaling molecules.
Cells, plasmids, and oligonucleotides
The complete complementary DNAs (cDNAs) of murine Tec, human cKit, and
human Dok-1 (a kind gift of N. Carpino, St Jude Children's Hospital,
Memphis, TN) were cloned in the expression vector pSG5 (Stratagene, La
Jolla, CA) and the retroviral vector pBabe.40 The
expression vectors encoding Tec mutants and Transient transfection and viral transduction
For viral transduction experiments, amphotropic Phoenix cells were cultured in 6-well dishes and, 3 hours later, the cells were transfected with the use of calcium phosphate. After 48 hours, cells were treated with mitomycin C (10 µg/mL) for 1 hour, washed 3 times, and washed 3 more times 4 hours later. F36P cells (2 × 105/mL) were added and co-cultured for 20 to 24 hours in RPMI/DMEM medium (50%/50%). F36P cells were removed carefully from the Phoenix cells and cultured in RPMI 1640 medium. To select for stable transfected cells, puromycin (2 µg/mL) was added 48 hours later. Immunoprecipitations, Western blotting, and antibodies After serum starvation for 16 hours, F36P, Mo7e, and TF-1 cells (30 × 106/mL) were stimulated with SCF (100 ng/mL; a generous gift of Amgen, Thousand Oaks, CA), EPO (5 U/mL; a generous gift of Janssen-Cilag, Tilburg, The Netherlands), or GM-CSF (50 ng/mL), or left unstimulated for 5 minutes (SCF) or 10 minutes (EPO and GM-CSF) at 37°C. Reactions were stopped by adding ice-cold phosphate-buffered saline (PBS). Cells were lysed in lysis buffer (1% NP-40, 20 mmol/L Tris-HCl, pH 8.0, 137 mmol/L NaCl, 10 mmol/L EDTA, and 10% glycerol), supplemented with Complete protease inhibitor mix [Roche], Pefablock [Merck, Darmstadt, Germany], and 1 mmol/L orthovanadate) on ice for 15 minutes and centrifuged at 4°C for 10 minutes at 15 000 rpm. Lysates of 15 × 106 cells were precleared with protein G beads (Sigma; St Louis, MO), incubated with the appropriate antibody (1 µg) for 90 minutes at 4°C, and protein G beads were added for an additional hour. Precipitates were washed 3 times with lysis buffer, subjected to SDS-polyacrylamide gel electrophoresis, and electrotransferred to nitrocellulose membrane (Schleicher & Schuell; Dassel, Germany). Membranes were blocked in 0.6% bovine serum albumin (BSA), incubated with appropriate antibodies, and developed with the use of enhanced chemoluminescence (ECL; NEN, Boston, MA).The following antibodies were used in this study: anti-Tec (06-561; Upstate Biotechnology, Lake Placid, NY), anti-TecSH3,31 anti-Dok-1 (M19; Santa Cruz, Santa Cruz, CA), antiphosphotyrosine (PY99; Santa Cruz), anti-HA (F-7; Santa Cruz), anti-Lyn (Transduction Laboratories, Lexington, KY), antiphospho-PKB (Ser473; New England BioLabs, Beverly, MA), anti-PKB (New England BioLabs), antiphospho-ERK1,2 (Thr202/Tyr204; E10; New England BioLabs), and anti-ERK1,2 (K-23; Santa Cruz). Glutathione S-transferase (GST)-pull down experiments For SH2 domain-inducing studies, SCF-stimulated Mo7e cells were lysed in immunoprecipitation buffer. Cleared lysates were incubated for 90 minutes with beads coupled to 10 µg of GST-SH2 fusion protein or to GST alone. Beads were washed 3 times with lysis buffer and resuspended in sample buffer. Proteins were separated by SDS-PAGE and blotted with antiphosphotyrosine antibody. The GST fusions with SH2 domains of the following proteins were used in this study: cAbl, CrkL, Fgr, Fps, GAP (both N- and C-terminal SH2 domains [N+C]), Grb14, Hck, Lyn, p85 subunit of PI3-K (SH2 N+C), PLC -1 (SH2 N+C), Shc, SHIP,
Src, Syk (SH2 N+C), Vav, and Yes.
Tec is activated in erythroid cells and complexes with various proteins after SCF addition Tec has previously been shown to be activated by SCF in the human megakaryocytic cell line Mo7e.18 To investigate whether Tec is specifically activated on cKit activation and to identify associating proteins, Tec was immunoprecipitated from the human erythroid progenitor cell line F36P and was stimulated with SCF, EPO, or GM-CSF, from Mo7e cells stimulated with SCF or GM-CSF, and from human erythroleukemic TF-1 cells stimulated with SCF. Subsequently, Tec and coprecipitating proteins were immunoblotted with antiphosphotyrosine antibody (PY99). As shown in Figure 1A, phosphorylation of Tec in F36P cells is only detected after SCF stimulation but not after incubation of the cells with EPO or GM-CSF, even though these cytokines induced tyrosine phosphorylation of multiple proteins (Figure 1A, lower panel). An in vitro kinase assay confirmed that the tyrosine phosphorylation of Tec correlated with enhanced kinase activity of Tec (not shown). Interestingly, a prominent phosphorylated protein of 62 kd as well as proteins of 145 to 150, 140, and 56 kd were precipitated with Tec after SCF induction. The proteins of 145 to 150 kd were identified as cKit by Western blot analysis (not shown). Because p56Lyn is known to associate with Tec,39 we tested whether the coprecipitating phosphoprotein of 56 kd was Lyn. Although the lower panel indeed shows that SCF treatment results in enhanced Tec-Lyn association, Lyn had a slightly faster mobility than p56. Therefore, the identities of p56 and p140 remain unknown.
Tec is also selectively phosphorylated by SCF in Mo7e cells (Figure 1B). In these cells, a similar pattern of coprecipitating proteins is observed, indicating that the same signaling complex is formed after cKit activation in F36P and Mo7e cells. Although less clear, cKit and p62 (marked with an asterisk) were also detected in the Tec precipitates of SCF-stimulated TF-1 cells. The signal in these cells is less intensive, most likely due to the lower level of activated cKit when compared with F36P and Mo7e, as is apparent from the whole cell lysate controls. The p62 coprecipitating with Tec is p62Dok-1 The most prominent tyrosine phosphorylated protein in Tec precipitates is a protein of 62 kd. Recently, Dok-1, a docking protein of 62 kd, was cloned from chronic myelogenous leukemia progenitor cells and from v-Abl-transformed B cells.22,44 In these cells, Dok-1 is constitutively phosphorylated. To examine whether Tec-associated p62 is identical to Dok-1, both Tec and Dok-1 were precipitated from SCF-stimulated F36P cells, blotted, and incubated with PY99. Figure 2A shows that p62 exactly comigrates with the slower migrating species of Dok-1. Strikingly, proteins of 140 and 56 kd coprecipitate with Dok-1, similar to what is observed when Tec is immunoprecipitated. These results suggest that (partially) identical complexes are precipitated with anti-Tec and anti-Dok sera. Reprobing of the blot with anti-Tec serum showed that Tec was present in the Tec immunoprecipitations (Figure 2A, lower panel). However, Tec was never detected in Dok-1 precipitations, possibly due to sterical hindrance of the antibody. Therefore, we assume that the coprecipitating p56 and p140 associate with Dok-1 rather than with Tec.
The anti-Dok-1 antibody could not be used in Western blot analysis but worked well in immunoprecipitations. To demonstrate that p62 is indeed Dok-1, lysates of SCF-stimulated F36P cells were first cleared with anti-Dok-1, followed by a Tec precipitation, and vice versa. If Tec and Dok-1 associate, the signal of p62/Dok-1 should be decreased in Tec- and Dok-1-precleared precipitates. In fact, significantly less p62 coprecipitates with Tec in lysates precleared from Dok-1 (Figure 2B, lanes 4 and 5), whereas there is almost no p62Dok remaining in a Dok-1 precipitate after the lysate was precleared with anti-Tec (Figure 2B, lanes 7 and 8). Together, these data are consistent with the notion that the Tec-interacting protein of 62 kd is identical to Dok-1. Tec and Dok phosphorylation depends on PI3-K activity Like Tec, Dok-1 also contains a PH domain at its N-terminus, suggesting that recruitment of both proteins to the cKit-signaling complex could depend on PI3-K activation. To inhibit PI3-K activity, both the fungal metabolite wortmannin (WM) and the synthetic inhibitor LY294002 (LY) were employed. Pretreatment of the cells with 20 nmol/L WM (20 minutes) or 15 µmol/L LY (60 minutes) was sufficient to almost completely block the SCF-induced phosphorylation of Tec and the coprecipitating Dok-1 (Figure 3A-B). As controls, phosphorylation of the classical PI3-K target, protein kinase B (PKB), and ERK were assessed. At 15 µmol/L LY, phosphorylation of PKB was completely blocked as determined with specific antibodies against phospho-PKB (Ser473; Figure 3B) and phospho-PKB (Thr308; not shown). In contrast, this concentration of LY had no effect on the SCF-induced activation of ERK1/2 (lower panel). These results demonstrate the specificity of the PI3-K inhibitors and suggest that PI3-K activation has to precede Tec/Dok phosphorylation, most likely to recruit these proteins to the signaling complex. In addition, because ERK1/2 phosphorylation is not affected by LY, it appears that SCF-induced activation of the Ras-MAP kinase pathway is independent of Dok-1 phosphorylation.
In addition to pharmacological inhibitors, a dominant negative PI3-K
construct ( Dok-1 is a substrate of Tec and Lyn in 293 cells To study the interaction of Tec and Dok-1, transient transfection experiments were performed in 293 cells as well as in COS cells. As shown in Figure 4A, immunoprecipitation of Tec from transfected cells results in the detection of a single phosphorylated protein, corresponding to Tec (lane 2). When Dok-1 is coexpressed (lane 3), a 62 kd phosphoprotein is coprecipitated, indicating that Tec and Dok-1 can form a complex in an overexpression system. However, relatively little phosphorylated Dok-1 is precipitated with Tec in this system when compared with F36P and Mo7e cells. Notably, coexpression of Dok-1 reproducibly enhanced the phosphotyrosine content of Tec (compare lanes 2 and 3), suggesting that Dok-1 enhances the auto- or cross-phosphorylation of Tec, or recruits an endogenous kinase that can phosphorylate Tec. The tyrosine kinase Lyn has been shown to phosphorylate Tec,37 and it was detected in a complex of Tec and Dok-1 in F36P cells (Figure 1A). Therefore, Lyn was coexpressed with Tec and Dok-1 in 293 cells. Lyn increased Tec phosphorylation (Figure 4A, compare lanes 2 and 4) and enhanced the intensity of Dok-1 detected by antiphosphotyrosine antibodies in a Tec immunoprecipitation (compare lanes 3 and 5). The latter may be due to Lyn-induced increased Dok-1 phosphorylation and/or increased stability of the Tec/Dok-1 complex. To examine an effect of Lyn on the Tec/Dok-1 interaction, Tec was coexpressed with a Dok-1 tagged at the N-terminus with the HA epitope, which allowed detection of total Dok-1 on Western blots. More HA-Dok was coprecipitated with Tec when Lyn was cotransfected (Figure 4B, lanes 3 and 4), suggesting that Lyn stabilizes the Tec/Dok-1 interaction. To analyze whether the association of Tec and Dok-1 is similarly enhanced through SCF-signaling, Tec was immunoprecipitated from F36P cells stably transfected with HA-Dok. Activation of cKit indeed increased the association of Dok-1 and Tec (Figure 4C). These data suggest that Lyn plays an important role in SCF-induced Tec/Dok-1 complex formation, which could be due to phosphorylation of Dok-1 that allows interaction of the Tec SH2 domain with the phoshotyrosines of Dok-1.
To examine whether Lyn is able to phosphorylate Dok-1 directly, Lyn and Tec were coexpressed with Dok-1 in COS and 293 cells. In COS cells, both Tec and Lyn induced massive Dok-1 phosphorylation, whereas they had an additive effect when cotransfected (Figure 4D, upper panel). Under these conditions, both Tec and Lyn coprecipitated with Dok-1 (indicated with arrows). In 293 cells, the SV40-driven promoter on the pSG5 plasmid is less active, resulting in a more modest expression of Tec and Lyn. In these cells, Dok-1 is a better substrate for Tec than for Lyn (Figure 4D, lower panel, lanes 3 and 4). Moreover, Lyn had no additive effect on Tec-mediated Dok phosphorylation (lane 5). Thus, although Lyn can phosphorylate Dok-1, its tyrosine phosphorylation appears to depend mainly on Tec activity. In conclusion, activation of cKit stabilizes the interaction between Tec and Dok-1, possibly by Lyn-mediated Dok-1 phosphorylation. Subsequently, Tec phosphorylates Dok-1 to yield highly tyrosine-phosphorylated Dok-1. Domains involved in Tec/Dok association Given the interaction observed between Tec and Dok-1, we next examined which domains were involved. Wild-type (wt) Tec and Tec deletion constructs (see Figure 5B for a schematic representation) were coexpressed with HA-Dok-1 in 293 cells. Subsequently, Tec precipitates were blotted and probed with anti-HA- and antiphosphotyrosine antibodies. Wt Tec, Tec SH3, and TecKD
bind equal amounts of HA-Dok-1 (Figure 5A, upper panel). In contrast,
TecPH is much more powerful in HA-Dok-1 binding, whereas no HA-Dok-1
is precipitated with TecTH and TecSH2. Similar results are
obtained when the blot is probed with PY99, ie, an equal Dok-1
phosphorylation by wt Tec and TecSH3, enhanced phosphorylation with
TecPH, and no phosphorylation with TecTH, TecSH2. The only
difference concerned TecKD (no kinase activity), which failed in
Dok-1 phosphorylation even though it precipitated HA-Dok-1, showing
that indeed Tec kinase activity is responsible for the phosphorylation
of HA-Dok-1. The detection of high levels of phosphorylated Dok-1 by
TecPH is most likely due to enhanced Dok-1 binding (upper panel).
Expression of Tec constructs was controlled with an antibody against
the TH domain, therefore, TecTH could not be detected (Figure 5A). Staining the blot with an antibody against the SH3 domain of Tec showed
that TecTH was expressed comparable to wt Tec (not shown). These
data show that 2 independent domains of Tec, the TH domain and the SH2
domain, interact with Dok-1.
Dok-1 is a docking protein that contains a PH domain, a
phosphotyrosine-binding (PTB) domain, 15 tyrosine residues, and 10 proline (PXXP) motifs. The phosphorylated tyrosines can act as SH2-binding sites, whereas the PXXP motifs mediate binding to SH3
domains. To roughly map the region(s) of Dok-1 that associate with Tec,
2 progressive deletion constructs were created (Figure 5C). In
HA-Dok SH2 domains of distinct signaling molecules bind to phosphorylated Dok-1 We demonstrated that SCF induces the complex formation of Dok-1 with Tec and Lyn and that Dok-1 is subsequently phosphorylated on tyrosine residues. Phosphorylated tyrosines act as binding sites for SH2-containing proteins. To determine which signaling intermediates could bind to phosphorylated Dok-1, GST fusion proteins that contained the SH2 domains from a range of signaling molecules were incubated with lysate from SCF-stimulated Mo7e cells. The SH2 domains of the Src family members Src, Fgr, Hck, and Yes; the tyrosine kinase cAbl; the adapter CrkL; rasGAP (SH2 N+C); the p85 subunit of PI3-K (SH2 N+C); PLC (SH2 N+C); and SHIP bind efficiently to phospho-Dok-1 (Figure
6). However, the SH2 domains of Fps,
Grb14, Shc, Syk (SH2 N+C), and Vav show little or no association with
Dok-1, illustrating a high level of substrate specificity. Precipitates
that contain Dok-1 also include proteins of 145 to 150, 140, and 56 kd,
although with different ratios. It cannot be excluded that some of the
GST-SH2 fusion proteins directly bind to cKit and thereby indirectly
precipitate Dok-1. However, these results strongly suggest that Dok-1
can recruit a variety of signaling proteins and, therefore, that Dok-1
may play an important role in SCF-mediated signaling.
Various studies have shown that SCF-mediated proliferation,
survival, adhesion, migration, and differentiation depend on PI3-K activity.16,24-26 PI3-K yields PIP3 in the cell membrane,
which can subsequently recruit PH domain-containing signaling
molecules.27 One such protein activated by SCF is the
tyrosine kinase Tec. We found that activation of cKit induces both Tec
activation and the formation of a complex of Tec with cKit, Lyn,
p62Dok-1, and 2 unidentified proteins of 56 and 140 kd, respectively.
By using pharmacological inhibitors and overexpression of
Tec and Dok-1 are partially associated in nonstimulated serum-starved F36P and Mo7e cells (Figure 1), indicating that the interaction is relatively stable in vivo. In cotransfection studies, however, the Tec/Dok-1 association is rather weak, although Dok-1 is heavily phosphorylated by Tec when coexpressed (Figure 4A and 4D, respectively). With the use of an HA-tagged Dok-1 construct, it was shown that cKit stimulation stabilizes the association between Tec and Dok (Figure 4C). This implies that additional proteins are necessary to allow the formation of a stable complex. Our results suggest that Lyn is such a protein (Figure 4B). Furthermore, Lyn also phosphorylates Dok-1 (Figure 4D), whereas the SH2 domain of Tec is crucial for its association with Dok-1 (Figure 5A). One possible mechanism to explain these results is that Lyn phosphorylates a subset of tyrosines in Dok-1, including the one(s) that mediate(s) the interaction with Tec. In turn, Tec may phosphorylate another subset of tyrosines of Dok-1. To further clarify the role of Tec in the phosphorylation of Dok-1 in vivo, we tried to stably transfect F36P cells with a Tec construct that lacked its kinase domain. However, in all clones obtained the expression level of this mutant was too low to act as a dominant negative. It can, therefore, not be excluded that Src family members fully account for the phosphorylation of Dok-1 in vivo. During our studies, Tec was identified as a possible kinase for CD28-mediated Dok-1 phosphorylation, whereas a Dok-related protein (Dok-R or p56Dok-2) has been reported as a direct target of Lyn.35,45 These data are consistent with the conclusion that Dok-1 and Dok-related proteins are substrates for both the Tec and Src families of tyrosine kinases. Dok-1 was first identified as a tyrosine-phosphorylated protein of 62 kd associated with p120-RasGAP in fibroblasts transfected with v-Src.46 In BCR-Abl-transformed cells, Dok-1 also binds to RasGAP in a tyrosine phosphorylation-dependent manner. However, RasGAP was not detected in Tec and Dok-1 precipitates, although RasGAP was detectable in RasGAP precipitates and in whole cell lysate controls (data not shown). In contrast, we detected a tyrosine-phosphorylated protein of 140 kd in Tec or Dok-1 precipitates of SCF-stimulated cells. Interestingly, a protein of about the same size was shown to bind to Dok-R in epidermal growth factor-stimulated cells.45 A protein that is activated by SCF and has a molecular weight of 150 kd is the inositol phosphatase SHIP-1. Therefore, we are currently investigating the role of this protein in the Dok-1 complex. In addition, the identity of p56 remains to be defined. Both p56Lyn and p52Shc are known to bind to Tec but have another electrophoretic mobility than Dok-bound p56 (data not shown). Still little is known about the physiological role of Dok-1. Dok-1 is directly associated with v-Abl and constitutively phosphorylated in chronic myelogenous leukemia cells.44,47 Furthermore, the extent of tyrosine phosphorylation of Dok-1 has been shown to correlate with the transforming capacities of a number of different oncogenes, including v-Src, v-Fps, v-Fms, and v-Abl.46 Because of the frequently noted correlation between constitutive tyrosine phosphorylation of Dok-1 and cellular transformation, it has been suggested that Dok-1 plays an important role in mitogenic signaling. This idea is in agreement with the observations that Dok-1 is phosphorylated in response to SCF and that SCF primarily serves as a potent proliferation factor in hematopoietic progenitor cells. The results of other studies have suggested that Dok-1 may also play a role in cellular migration responses. Recently, Noguchi et al48 showed that overexpression of wt Dok-1 enhanced insulin-induced migration, but this was not observed with DokY361F, a Dok-1 mutant unable to bind Nck. Nck is an adapter molecule that links receptors to p21cdc42/Rac-activated kinase and the Wiskott-Aldrich syndrome protein-interacting protein complex, all of which contribute to changes in the actin cytoskeleton.49,50 Furthermore, Rac has been shown to be activated by cKit via a Src- and PI3-K-dependent mechanism and plays an important role in SCF-induced proliferation of bone marrow-derived mast cells.24 One may speculate that the Tec/Dok-1 complex recruits and activates the components that regulate the (de)polymerization of actin filaments, thereby regulating cell migration. Dok-1 contains a PTB domain, 15 tyrosine residues, and 10 PXXP motifs
and has an overall structure related to the insulin-receptor substrates
1 to 4 and GAB, all of which serve as docking molecules.51 By recruiting subsets of signaling molecules into an activated receptor
complex, docking molecules play a key role in the coordination of the
cellular response. Others22,48 have shown that p120RasGAP and Nck directly bind to Dok-1, whereas our results indicate that Tec
as well as at least 2 unidentified proteins associate to Dok-1. We
further showed that many SH2 domains of proteins functioning in
different signaling routes form a complex with Dok-1. These include the
SH2 domains of Abl, CrkL, PLC
We would like to thank Nick Carpino, Paul Coffer, and Rolf de Groot for the kind gift of materials and plasmids, and Ivo Touw and Alister Ward for critically reading the manuscript and for useful discussions.
Submitted December 27, 1999; accepted July 18, 2000.
Supported by a grant from the Dutch Cancer Society (EUR 95-1021) and by a fellowship of the Dutch Academy for Arts and Sciences (KNAW) to M.v.L.
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked "advertisement" in accordance with 18 U.S.C. section 1734.
Reprints: Thamar van Dijk, Institute of Hematology, Erasmus University Rotterdam, PO Box 1738, 3000 DR Rotterdam, The Netherlands; e-mail: vandijk{at}hema.fgg.eur.nl.
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M. J. Wick, L. Q. Dong, D. Hu, P. Langlais, and F. Liu Insulin Receptor-mediated p62dok Tyrosine Phosphorylation at Residues 362 and 398 Plays Distinct Roles for Binding GTPase-activating Protein and Nck and Is Essential for Inhibiting Insulin-stimulated Activation of Ras and Akt J. Biol. Chem., November 9, 2001; 276(46): 42843 - 42850. [Abstract] [Full Text] [PDF]
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Top
Abstract
Introduction
). Tec and Dok-1 immunoprecipitates were analyzed
by Western blotting with antiphosphotyrosine (top panels) and anti-Tec
(lower left panel). Tyrosine phosphorylation of Tec and Dok-1 can be
blocked by coexpression of dominant negative PI3-K (
