|
|||||||||
|
|
|
|
|
|
|
|
|
|
|
PHAGOCYTES
From the Granulocyte Research Laboratory, Departments
of Hematology and Clinical Biochemistry, Copenhagen University
Hospital, Denmark; the Department of Infectious Diseases,
Linköping University, Sweden; the Division of Cell Biology,
Netherlands Cancer Institute, Amsterdam, The Netherlands; and the
Department of Pulmonology, Leiden University Medical Center, Leiden,
The Netherlands.
Cathelicidins are a family of antimicrobial proteins found in the
peroxidase-negative granules of neutrophils. The known biologic functions reside in the C-terminus, which must be cleaved from the
holoprotein to become active. Bovine and porcine cathelicidins are
cleaved by elastase from the azurophil granules to yield the active
antimicrobial peptides. The aim of this study was to identify the
physiological setting for cleavage of the only human cathelicidin, hCAP-18, to liberate the antibacterial and cytotoxic peptide LL-37 and
to identify the protease responsible for this cleavage. Immunoelectron microscopy demonstrated that both hCAP-18 and azurophil granule proteins were present in the phagolysosome. Immunoblotting revealed no
detectable cleavage of hCAP-18 in cells after phagocytosis. In
contrast, hCAP-18 was cleaved to generate LL-37 in exocytosed material.
Of the 3 known serine proteases from azurophil granules, proteinase 3 was solely responsible for cleavage of hCAP-18 after exocytosis. This
is the first detailed study describing the generation of a human
antimicrobial peptide from a promicrobicidal protein, and it
demonstrates that the generation of active antimicrobial peptides from
common proproteins occurs differently in related species.
(Blood. 2001;97:3951-3959) Human polymorphonuclear neutrophilic leukocytes
(PMNs) contain a variety of antibiotic proteins.1 These
are mainly localized in granules.2 When the granules are
mobilized, these proteins are released to the exterior or into the
phagolysosome, where the contents of the peroxidase-negative and
peroxidase-positive granules of neutrophils meet and cooperate in the
killing of microbes.
In human PMNs, most bactericidal proteins are localized in the
azurophil granules2 Cathelicidins are a family of antimicrobial and endotoxin-binding
proteins found in peroxidase-negative granules of vertebrate neutrophils.7 Members of this protein family share a
highly conserved N-terminus of 12 kd, named cathelin after a protein isolated from porcine neutrophils.8
The cathelicidins are synthesized as preproproteins.7
After removal of the signal peptide, they are stored in granules as
inactive proforms. The active biologic domains of the cathelicidins generally reside in the C-terminus. The C-terminal antibacterial peptides are activated when cleaved from the proforms of the
cathelicidins by serine proteases from azurophil
granules.9-11 The C-termini of the cathelicidins vary
greatly in amino acid sequence and structure, ranging from proline- and
arginine-rich sequences to sequences forming amphipathic Porcine and bovine neutrophils contain a variety of cathelicidins,
whereas hCAP-18 is the only human cathelicidin.12-16
hCAP-18 is a major protein in specific granules of
neutrophils,17 but it is also present in subpopulations of
lymphocytes and monocytes,18 in squamous
epithelia,19 epididymis and seminal plasma,20 in the lung,21,22 and in keratinocytes during
inflammatory skin diseases.23 Plasma contains a high
concentration of hCAP-18 bound to lipoproteins.24 The
antibacterial C-terminus of hCAP-18, LL-37, has been isolated
from exocytosed material from neutrophils.15 It shows
broad antimicrobial activity toward both gram-negative and
gram-positive bacteria,25 has synergistic antibacterial effects with the defensins,26 and is a chemotactic
agent for neutrophils, monocytes, and T cells using the formyl peptide
receptor-like 1 receptor.27 However, LL-37 is also
cytotoxic toward mammalian cells.28
In bovine and porcine neutrophils, the antimicrobial peptides are
liberated by elastase-mediated cleavage of
cathelicidins.10,11 However, the potential cleavage site
of hCAP-18 is different from the cleavage site of bovine and porcine
cathelicidins (Figure 1). The aims of
this study were to identify the biologic settings in which LL-37 is
cleaved from hCAP-18 and to identify the protease responsible for this
cleavage.
Materials
Anti-proteinase 3 antibodies and proteinase 3 were generously provided
by Jörgen Wieslander (Wieslab AB, Lund, Sweden). Human leukocyte
elastase, cathepsin G, and SLPI were purchased from ICN Biomedicals
(Costa Mesa, CA). Antielastase antibodies were from Biodesign
International (Kennebunk, ME). All other antibodies were purchased from
DAKO A/S (Glostrup, Denmark).
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and
immunoblotting
Isolation of neutrophils from peripheral blood Human neutrophils were isolated from freshly prepared buffy coats or from healthy donors as described.32 Briefly, after sedimentation with 2% Dextran T-500 (Amersham Pharmacia Biotech, Uppsala, Sweden) in isotonic NaCl, the leukocyte-rich supernatant was pelleted and resuspended in saline for subsequent centrifugation on Lymphoprep (Nycomed Pharma A/S, Oslo, Norway) at 400g for 30 minutes for the removal of lymphocytes and monocytes. Remaining erythrocytes were lysed in ice-cold de-ionized water for 30 seconds. Tonicity was restored by the addition of 1 vol of 1.8% NaCl. Cells were washed once and resuspended in the desired buffer. With the exception of Dextran sedimentation, all steps were carried out at 4°C.Isolation of exudate neutrophils from skin window chambers Exudate neutrophils were isolated from skin window chambers placed on the forearms of healthy human donors, as described.33,34 Briefly, chambers with 3 0.6-mL wells covering the lesions were used. They were filled with autologous serum and incubated for 18 hours. Chambers were then emptied, washed, and filled with fresh autologous citrated plasma. Neutrophils were allowed to accumulate in the chambers for 7 hours. Cells were harvested, pelleted by centrifugation, washed once, and resuspended in the desired buffer. More than 95% of the harvested cells were neutrophils.Purification of hCAP-18 from neutrophils Neutrophils were disrupted by nitrogen cavitation after the addition of 5 mM di-isopropyl fluorophosphate (Sigma, St Louis, MO). Postnuclear supernatants were loaded on 2-layer gradients (1.05/1.12 g/mL) of Percoll (Amersham Pharmacia Biotech).35 This resulted in 3 visible bands. Starting at the bottom, the bands are designated the -band, containing azurophil granules; the  -band,
containing specific and gelatinase granules; and the  -band,
containing plasma membranes and secretory vesicles.
The Isolated specific granule proteins were subjected to cation exchange chromatography on a MonoS column using ÄKTA-FPLC (Amersham Pharmacia Biotech AB). Most of the bound material was eluted with 1 M NaCl, 9.5 mM phosphate, pH 7.4. hCAP-18 was subsequently eluted with 10 mM NaOH, 140 mM NaCl. Immunoblotting with anti-hCAP-18 antibodies of the eluted hCAP-18 showed one band of the appropriate molecular mass. For cleavage experiments with purified proteases and amino acid sequence analysis, hCAP-18 was purified from specific granules on an anti-hCAP-18 antibody column as previously described.24 Isolation of azurophil granule proteins from neutrophils Neutrophils were subjected to nitrogen cavitation and subcellular fractionation as described above but without protease inhibitors. After the removal of Percoll from the-band containing
the azurophil granules, the granules were freeze-thawed 5 times in 1 M
NaCl. Membranes were pelleted by ultracentrifugation, and the
supernatant containing the matrix proteins of azurophil granules was
harvested and stored at  80°C until further use.
Exocytosis and phagocytosis experiments Isolated neutrophils, freshly prepared from peripheral blood or skin windows of healthy donors, were resuspended in Krebs Ringer phosphate (10 mM NaH2PO4/Na2HPO4, 130 mM NaCl, 5 mM KCl, 0.95 mM CaCl2, 5 mM glucose) at a concentration of 107 cell/mL. Cells were preincubated at 37°C for 5 minutes and then stimulated with 1 µM ionomycin (Calbiochem, La Jolla, CA), 10 8 M formyl
methionyleucylphenylalanine (fMLP; Sigma), or IgG-coated latex
beads for 20 minutes at 37°C. Stimulation was stopped by the addition
of 2 vol ice-cold buffer and subsequent pelleting by centrifugation.
The supernatant containing the exocytosed material was analyzed by
enzyme-linked immunosorbent assay (ELISA) or immunoblotting.
After stimulation, aliquots of the cells were either used for quantification of granule proteins by ELISA or resuspended to a concentration of 1 × 106 cells/mL and precipitated with 5% trichloroacetic acid (final concentration). The pellet was washed 5 times with acetone and resuspended in Laemmli sample buffer for analysis by SDS-PAGE and immunoblotting. Remaining cells were fixed for electron microscopy. Preparation of exocytosed material for cleavage experiments Neutrophils (3 × 107 cells/mL) were stimulated to exocytosis by 1 µM ionomycin as described above. After stimulation, the cells were placed on ice for 10 minutes and subsequently pelleted by centrifugation. The supernatant was frozen at20°C until further experiments. Endogenous hCAP-18 was subsequently removed from the
exocytosed material by affinity chromatography on an anti-hCAP-18 antibody column. After affinity chromatography, the exocytosed material
was immediately used as a source of proteases for cleavage of
hCAP-18.
Cleavage experiments Intact hCAP-18 isolated from specific granules by ÄKTA-FPLC was incubated with exocytosed material from neutrophils, azurophil granule proteins, or purified proteases at 37°C for 30 minutes. The sample was subsequently boiled in Laemmli sample buffer and run on a SDS-PAGE followed by immunoblotting.Amino acid sequence analysis Amino acid sequence was analyzed on the PVDF-blotted protein in a 494 A Procise Protein Sequencer (PerkinElmer, Palo Alto, CA) using the blot cartridge and PVDF cycles. All reagents and solvents were supplied by PerkinElmer.Immunoprecipitation Antibodies against elastase, cathepsin G, proteinase 3,1-antitrypsin, and normal rabbit immunoglobulins were
incubated with Protein A Sepharose (Pharmacia) for 30 minutes at room
temperature in PBS (pH 7) with 0.5 M NaCl. Sepharose particles were
subsequently washed 7 times in PBS with 0.5 M NaCl to remove unbound
antibodies; this was followed by incubation with exocytosed material at
4°C for 2 hours. Sepharose particles were pelleted by centrifugation. Supernatants were aspirated and immediately used for cleavage experiments.
Immunoelectron microscopy Cells were fixed in a mixture of 0.5% glutaraldehyde and 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.2) for 2 hours at room temperature. They were then stored in 4% paraformaldehyde at 4°C until they were processed for ultrathin cryosectioning. For single labeling, cryosections were incubated with rabbit anti-hCAP-18; this was followed by 10-nm protein A-conjugated colloidal gold. For double labeling, the sections were first incubated with mouse monoclonal anti-human myeloperoxidase (CLB; Amsterdam, The Netherlands) followed by rabbit anti-mouse IgG and 5-nm protein A gold, and then they were treated with 1% glutaraldehyde for 10 minutes to prevent interference between the different antibody gold complexes in the sections.36 They were further incubated with rabbit anti-hCAP-18 followed by 10-nm protein A-conjugated colloidal gold (5-nm and 10-nm protein A-conjugated gold; EM Laboratory, Utrecht University, The Netherlands). After immunolabeling, the cryosections were embedded in a mixture of methylcellulose and uranyl acetate and examined with a Philips CM 10 electron microscope (Eindhoven, The Netherlands). For controls, the primary antibody was replaced by a nonrelevant murine or rabbit antiserum, respectively.Quantitation of proteins Myeloperoxidase, hCAP-18, and gelatinase were measured by ELISA as previously described.29,37,381-Antitrypsin, elastase, cathepsin G, and proteinase 3 were quantitated by semiquantitative ELISA. Anti-proteinase 3 and
anti-cathepsin G antibodies were isolated from antiserum using
ÄKTA-FPLC. All the antibodies were biotinylated as
described.39
Samples were diluted in 50 mM Na2CO3/NaHCO3 buffer, pH 9.6, and incubated in 96-well flat-bottom immunoplates (Nunc, Roskilde, Denmark) overnight at room temperature. Unspecific binding was blocked by incubation with 200 µL/well dilution buffer (0.5 M NaCl, 3 mM KCl, 8 mM Na2HPO4/KH2PO4, 1% BSA (Sigma), 1% Triton X-100, pH 7.2) for 1 hour. Biotinylated antibodies against the above-mentioned antigens were diluted in dilution buffer and incubated for 1 hour. Horseradish peroxidase-labeled avidin (DAKO) was diluted 1500-fold in dilution buffer and incubated for 1 hour. Plates were washed 3 times in washing buffer (0.5 M NaCl, 3 mM KCl, 8 mM Na2H4/KH2PO4, 1% Triton X-100, pH 7.2) after each incubation using a SkanWasher 410 (Skatron, Roskilde, Denmark). Plates were washed once in substrate buffer (0.1 M sodium phosphate, 0.1 M citric acid, pH 5.0) before color development and then incubated with substrate buffer containing 0.04% o-phenyl-diamine (Kem-En-Tec, Copenhagen, Denmark) and 0.03% H2O2. Unless otherwise stated, 100 µL was added to each well at each incubation step. Color development was stopped by the addition of 100 µL 1 M H2SO4, absorbance measured at 492 nm in a Multiscan Plus ELISA Reader (Labsystems, Helsinki, Finland). A standard curve of serial dilutions of exocytosed material from neutrophils was used. Activity of exocytosed elastase and cathepsin G Freshly prepared exocytosed material from ionomycin-stimulated neutrophils (5 × 107 cells/mL) was incubated with specific nitroanilide substrates for elastase (N-methoxysuccinyl-ala-ala-pro-val p-nitroanilide; Sigma) or cathepsin G (N-methoxysuccinyl-ala-ala-pro-met p-nitroanilid; Sigma). The amount of free nitroanilide was quantitated by measurement of the absorbance at 410 nm.Preparation of lipoprotein-bound hCAP-18 Purified hCAP-18 was incubated with plasma for 2 hours at 37°C. Plasma was then subjected to molecular-sieve chromatography on a Superose 12 column using ÄKTA-FPLC. The high molecular peak fraction of hCAP-18 containing lipoprotein-bound hCAP-18, as previously described,24 was used for further cleavage experiments.
To investigate whether hCAP-18 is cleaved in the phagocytic
vacuole, neutrophils from peripheral blood and from skin windows were
isolated and stimulated to phagocytosis by immunoglobulin-coated latex
beads. After phagocytosis, the cells were fixed for electron microscopy
or pelleted and resuspended in 0.9% NaCl followed by TCA-precipitation. Immunoelectron microscopy demonstrated that the PMNs
had phagocytosed the latex beads and that hCAP-18 was found both in the
specific granules and in the phagolysosomes (Figure
2A). In single sections from 106 exudate
neutrophils from skin windows, phagolysosomes were found in 103 cells.
In blood neutrophils, phagolysosomes were found in only 1 of 103 cells. Double-immunogold labeling of hCAP-18 and myeloperoxidase was performed
to demonstrate that both azurophil and specific granules had fused with
the phagolysosome (Figure 2B). Thirty-three phagolysosomes were
examined for the presence of myeloperoxidase and hCAP-18 in 7 sections
from different exudate neutrophils from skin windows. Twenty-seven
phagolysosomes were labeled with both myeloperoxidase and hCAP-18.
Three were labeled only for myeloperoxidase and 3 only for hCAP-18.
Because only one section was examined for each phagolysosome, it cannot
be ruled out that those positive only for one marker would have been
positive for both markers in another section of the same phagolysosome.
Thus, most phagolysosomes contained both hCAP-18 and azurophil granule
proteins. TCA precipitates from neutrophils after phagocytosis were
analyzed by SDS-PAGE, and then they were immunoblotted with
anti-hCAP-18 antibodies. Despite the "priming" of phagocytosis in
cells from skin windows, no intracellular cleavage of hCAP-18 was found
after phagocytosis of latex beads (Figure 3A, lane
c). The same result was found in blood
neutrophils (Figure 3B, lane c). As expected, unperturbed cells and
cells stimulated to exocytosis by fMLP and ionomycin showed no cleavage
of hCAP-18 (see Figure 3A-B, lanes a, b, and d). Control experiments
ascertained that TCA-precipitation did not influence the detection of
the low-molecular-weight fragments by immunoblotting and that the
cleavage of hCAP-18 by serine proteases from azurophil granules was not
inhibited by immunoglobulin-coated latex beads (data not shown). To
validate that the lack of detectable cleavage of hCAP-18 in cell
lysates after phagocytosis did not result from insufficient
degranulation of hCAP-18 into the phagocytic vacuole, the
immunogold-labeled hCAP-18 was counted in granules and phagolysosomes.
More than 50% of the labeled hCAP-18 was present in the phagolysosome
in the neutrophils harvested from skin windows and stimulated to
phagocytosis by latex beads (Table 1).
Although this was a semiquantitative measure of degranulation into the phagolysosome, it demonstrated that a substantial part of the hCAP-18
in these cells was localized to the phagolysosome. Phagocytosis experiments with serum-treated zymosan particles performed with neutrophils from skin windows and peripheral blood gave similar results
(data not shown). Thus, cleavage of hCAP-18 was not detectable in the
phagocytic vacuole.
Exocytosis experiments Neutrophils from peripheral blood or from skin chamber windows were stimulated to exocytosis by different secretagogues (Table 2). The exocytosed material was analyzed by immunoblotting with anti-hCAP-18 antibodies. Significant cleavage of hCAP-18 was only detected in the exocytosed material from ionomycin-stimulated neutrophils (Figure 4A-B, lane d). We have previously demonstrated that the 14-kd fragment of hCAP-18 in the exocytosed material is cathelin and that the 4-kd fragment represents the noncathelin C-terminus of hCAP-18.24 The absolute concentrations of azurophil granule proteins were highest in the exocytosed material from ionomycin-stimulated cells (in particular from blood neutrophils) (Table 2). The absolute concentration of azurophil markers correlated with the degree to which hCAP-18 was cleaved. This indicates that the concentration of protease in the medium determines whether hCAP-18 is cleaved. Prolonged incubation (1 hour) of neutrophils did not give rise to further cleavage of hCAP-18 in the exocytosed material (data not shown). When neutrophils were stimulated by fMLP at a cell concentration of 3 × 108 cells/mL, the hCAP-18 in the exocytosed material was cleaved (Figure 4C). Thus, cleavage occurs even after stimulation with weak secretagogues if the cell concentration is high enough, indicating that hCAP-18 cleavage may take place during the accumulation of neutrophils in acute inflammation.
Cleavage experiments with serine proteases Immunoblotting of TCA-precipitated cells showed that hCAP-18 exists intracellularly as a holoprotein, as previously described,17 indicating that cleavage of hCAP-18 is performed by a protease not present in the same subcellular compartment as hCAP-18. Thus, it seemed likely that hCAP-18 was cleaved by a serine protease from azurophil granules, as described for bovine and porcine cathelidicins.10,11 Incubation with azurophil granule proteins resulted in the cleavage of hCAP-18 (Figure 5, lane b), which could be inhibited both by phenylmethylsulfonyl fluoride and by aprotinin (Figure 5, lanes c, d), showing that serine proteases were responsible for the cleavage of hCAP-18 by the azurophil granule proteins. However, the cleavage of hCAP-18 by azurophil granule proteins did not resemble the cleavage observed after exocytosis. There were clearly 2 bands of approximately 14 kd rather than only one band in the exocytosed material.
To further characterize the cleavage pattern of hCAP-18 by azurophil
granule proteases, immunoblotting was performed with a monoclonal
antibody toward the antimicrobial domain of hCAP-18, LL-37 (Figure
6). Immunoblotting of exocytosed material
from neutrophils showed one band of 18 kd (the holoprotein) and one
band of 4 kd (LL-37) (Figure 6, lane b). As anticipated, the 14-kd band
of cathelin was not recognized by the monoclonal antibody. In contrast, a band of 14 kd was detected by the monoclonal antibody against LL-37
when hCAP-18 was cleaved by extracts of azurophil granules (Figure 6,
lane c). Thus, at least some of the cleavage of hCAP-18 by serine
proteases from azurophil granules occurs at a location different from
that between the cathelin part and LL-37, resulting in a 14-kd fragment
that contains parts of the cathelin and of the LL-37 moiety.
hCAP-18 was then incubated with each of the 3 known serine proteases in azurophil granules, and immunoblotting was performed with the monoclonal anti-LL-37 antibody. All 3 proteases were capable of cleaving hCAP-18 (Figure 6, lanes d-f). Cleavage of hCAP-18 by elastase and cathepsin G resulted in clearly visible bands at 14 kd not seen in the exocytosed material. Incubation of hCAP-18 with different concentrations of elastase or cathepsin G did not give rise to the cleavage pattern observed in the exocytosed material (data not shown). Cleavage by proteinase 3 gave rise only to LL-37 (Figure 6, lane f), similar to what was observed in the exocytosed material. Inhibition and immunoprecipitation experiments with exocytosed material from neutrophils Because the pattern of hCAP-18 cleavage by azurophil granule extracts was different from the cleavage pattern of endogenous hCAP-18 in the exocytosed material, experiments were performed with the exocytosed material from ionomycin-stimulated neutrophils to identify the protease responsible for cleaving hCAP-18. Endogenous hCAP-18 in the exocytosed material was removed by affinity chromatography on an anti-hCAP-18 antibody column (in the presence of 0.5 M NaCl to prevent unspecific absorption to the column), and the exocytosed material was then incubated with purified hCAP-18. This resulted in cleavage of hCAP-18 that was similar to that of endogenous hCAP-18, originally observed in the exocytosed material, when immunoblotting was performed with monoclonal antibody (Figure 6, lane g) and with polyclonal antibodies (Figure 7, lane b). The cleavage of hCAP-18 by proteins in the exocytosed material was totally inhibited by the elastase inhibitor (N-methoxy-succinyl-ala-ala-pro-val chloromethyl ketone [CMK]), but not by chymostatin (an inhibitor of chymotrypsin-like proteases such as cathepsin G) or secretory leukocyte protease inhibitor (SLPI) (a known inhibitor of elastase and cathepsin G) (Figure 7A, lanes c-e).
We then examined the susceptibility of purified serine proteases to
these inhibitors. The cleavage of hCAP-18 by elastase (Figure
8A, lane b) was totally inhibited by CMK
and SLPI but not by chymostatin (Figure 8A, lanes c-e). Cleavage by
cathepsin G (Figure 8B, lane b) was totally inhibited by chymostatin
and SLPI but not by CMK (Figure 8B, lanes c-e). Cleavage by proteinase 3 (Figure 8C, lane b) was totally inhibited by CMK but not by chymostatin or SLPI (Figure 8C, lanes c-e).
Thus, both the cleavage pattern of hCAP-18 and the results of the inhibition experiments in the exocytosed material are similar to those obtained with purified proteinase 3. To validate the experiments with exocytosed material, the serine proteases were quantitated by ELISA. The removal of hCAP-18 by affinity chromatography did not increase the concentration of proteinase 3 relative to the concentrations of elastase and cathepsin G (data not shown). Proteinase 3 was then immunoprecipitated from the exocytosed material
before incubation with hCAP-18. Immunoprecipitation with preimmune
rabbit antibodies, antielastase antibodies, or anti-cathepsin G
antibodies did not inhibit the cleavage of hCAP-18 (Figure
9, lanes b-d) in the exocytosed material,
whereas there was no cleavage of hCAP-18 after immunoprecipitation of
proteinase 3 (Figure 9, lane e). Measurements in the supernatants after
immunoprecipitation showed specific immunoprecipitation of proteinase 3 but no precipitation of elastase or cathepsin G after
immunoprecipitation of proteinase 3. Proteinase 3 was not precipitated
by antielastase or anti-cathepsin G antibodies (data not shown). The
specificity of the anti-proteinase 3 antibodies was validated by
immunoblotting. Before immunoblotting with anti-proteinase 3 antibodies, 1.25 µg purified elastase, cathepsin G, and proteinase 3 were run in separate lanes on SDS-PAGE. Reactivity was found only in
the lane with proteinase 3 (data not shown). Thus, proteinase 3 was
solely responsible for the cleavage of hCAP-18 in the exocytosed
material.
Because of the in vitro activity of elastase and cathepsin G toward hCAP-18, we examined whether these proteases were inhibited in vivo after exocytosis by 2 inhibitors reported to be exocytosed from human neutrophils. SLPI is reported to be a major protein in the neutrophil cytosol and to be exocytosed from human neutrophils.40 It inhibits elastase and cathepsin G but not proteinase 3. Thus, exocytosed SLPI could prevent the cleavage of hCAP-18 by elastase or cathepsin G in the exocytosed material from neutrophils. However, we were not able to detect any significant amounts of SLPI in unperturbed neutrophils or in the exocytosed material (O.E.S., N.B., P.S.H., unpublished observation, July 1999).
The activities of elastase and cathepsin G were then measured in the exocytosed material from neutrophils (5 × 108 cells/mL) using specific nitroanilide substrates. These experiments were performed in the presence and absence of SLPI to validate that the measured activity was not caused by proteinase 3. Absorbance measured in the presence of the elastase substrate was 3.23 compared to 0.43 when SLPI was added before incubation with the substrate; the corresponding values in the experiment with cathepsin C substrate were 1.42 and 0.26. Measured activities in these experiments were greater than those necessary in the in vitro experiments for the cleavage of hCAP-18 by isolated elastase or cathepsin G. Thus, both elastase and cathepsin G are present as active enzymes in the exocytosed material from neutrophils. Identification of the C-terminal fragments after cleavage of hCAP-18 To further validate that LL-37 was liberated by proteinase 3- mediated cleavage of hCAP-18, purified hCAP-18 was cleaved by incubation with proteinase 3. The sample was run on SDS-PAGE and blotted to a PVDF membrane, and the low-molecular-mass fragment was analyzed by N-terminal amino acid sequencing of the first 10 residues. These were identified as (L)LGDFFRKSK, consistent with LL-37. Because of contamination, the identity of the first residue could not be unequivocally determined.Influence of binding to lipoproteins We have previously shown that hCAP-18 circulates in plasma in a high concentration bound to lipoproteins.24 We therefore investigated whether lipoprotein-bound hCAP-18 was susceptible to cleavage by proteinase 3. Plasma was incubated with purified hCAP-18 and was subjected to gel filtration. After gel filtration, the peak fraction of hCAP-18 bound to lipoproteins of very low density and low density was incubated with proteinase 3. The lipoprotein-bound hCAP-18 was still susceptible to cleavage by proteinase 3 (Figure 10). Thus, the association to lipoproteins does not prevent the cleavage of hCAP-18.
The antibacterial peptide LL-37 is cleaved from the human cathelicidin hCAP-18 between an alanyl and a leucyl residue. This site differs from the cleavage sites in the bovine and porcine cathelicidins, which are cleaved by elastase at elastase-cleavage sites (Figure 1). Most notably, the basic arginyl residue after the cleavage site is substituted with the small aliphatic leucyl residue, and the traditional valyl residue just before the cleavage site is substituted with an alanyl residue. Leukocyte elastase prefers to cleave at a valyl rather than at an alanyl residue.43 Proteinase 3, on the other hand, prefers to cleave between 2 small aliphatic amino acids such as Ala-Leu,42 as found in the cleavage site of hCAP-18. The cleavage of hCAP-18 by proteinase 3 is a specific cleavage between the antimicrobial peptide and the cathelin part with no further degradation of the cathelin part. Similar specific cleavage of the porcine cathelicidin protegrin 3 is mediated by elastase.11 In contrast to the porcine cathelicidins, not all the bovine cathelicidins contain a valyl residue at the putative cleavage site.7 Thus, it remains to be seen whether some of the bovine cathelicidins are cleaved by proteases other than elastase. In mice44,45 and rabbits,46 the putative cleavage sites of the cathelicidins do not resemble those in human, porcine, or bovine cathelicidins. The specific proteases responsible for cleavage of these cathelicidins remain to be characterized. Cathelicidin genes are composed of 4 exons and 3 introns. There is great similarity between the first 3 exons encoding the conserved cathelin part between different cathelicidins but no homology in the fourth exon encoding the active antimicrobial domain and the putative cleavage site.15,16,47-50 Cleavage of hCAP-18 by proteinase 3 demonstrates that the cleavage site is a functional variable part of the cathelicidins, together with the antimicrobial domain, and that the members of the cathelicidin family are activated by different proteases in related species. Thus, during evolution the variable biologic functions of the cathelicidins have been changed solely by alterations in the fourth exon. The 3 known serine proteases in azurophil granules In our in vitro experiments, proteinase 3 did not seem to be as active toward hCAP-18 as elastase is toward bovine10 and porcine cathelicidins.11 The interesting question is whether hCAP-18 is processed extracellularly in vivo to a lesser extent by proteinase 3 than the bovine and porcine cathelicidins processed by elastase. Comparison is difficult because of different experimental conditions used (including different types of antibodies). Our polyclonal anti-hCAP-18 antibodies seemed to overestimate the amount of holoprotein, and the monoclonal anti-LL37 seemed to overestimate the amount of LL-37. However, we have previously blocked the binding of the polyclonal antibodies to the cathelin part of hCAP-18 by adding recombinant cathelin to the primary antibodies24; this is probably the best way to estimate the amount of LL-37 compared to holoprotein. In an experiment in which 1.4 × 107 neutrophils/mL was stimulated with ionomycin, we estimated that approximately 95% of the holoprotein was processed to LL-37.24 Even though hCAP-18 seemed to be activated extracellularly to a lesser extent than the bovine and porcine cathelicidins in vivo,9,11,51 most of the secreted hCAP-18 was processed to LL-37 if a sufficient amount of proteinase 3 was present extracellularly. Cleavage by proteinase 3 may be functionally significant for the
hCAP-18 expressed in nonhematopoietic tissues, such as
lung,21,22 skin,23 and
epididymis.20 Pulmonary monocytes from patients with
cystic fibrosis express proteinase 3,52 and the levels of
proteinase 3 activity are greater than those of elastase in the sputum
from patients with cystic fibrosis who have chronic lung
infections.53 SLPI is assumed to play an important role in
the protection against the leukocyte proteases, elastase, and cathepsin G and in the mucosa at various sites Conditions in the phagocytosis experiments were made optimal to positively demonstrate a cleavage of hCAP-18 in the phagolysosome. After phagocytosis, the cells were TCA-precipitated to avoid cleavage of hCAP-18 during further processing of the cells for immunoblotting. Control experiments with exocytosed material from neutrophils demonstrated that TCA precipitation did not influence the detection of low-molecular-weight fragments after the cleavage of hCAP-18. Furthermore, we used exudate neutrophils from skin windows. These "primed" cells are the closest experimental correlate to the neutrophils active in the tissues.33,34 Indeed, exudate neutrophils from skin windows were significantly more active in phagocytosing the latex beads than neutrophils from peripheral blood. Immunoglobulins were used as opsonizing ligands because they optimize the incorporation of specific granules (and, thus, hCAP-18) into the phagolysosome.57 Immunoglobulin-coated latex particles were found not to inhibit the cleavage of hCAP-18 by the serine proteases from azurophil granules. Furthermore, a substantial amount of the hCAP-18 in the cells was present in the phagolysosome, and azurophil granule constituents were found together with hCAP-18 in most examined phagolysosomes. Even very limited degranulation of azurophil granules into the phagolysosome would generate much higher concentrations of azurophil granule proteases in the phagolysosome than found extracellularly because of the much smaller volume of the phagolysosome. Yet, no cleavage of hCAP-18 was observed. Additional experiments were performed with serum-treated zymosan particles with similar results. We cannot completely rule out that small undetectable amounts of LL-37 are generated in the phagolysosome, but our data do show that phagocytosis, during which specific and azurophil granules fuse with the phagolysosome, is insufficient for the generation of significant amounts of LL-37. The main function of hCAP-18, therefore, seems to be extracellular, where LL-37 also acts as a chemotactic agent for neutrophils, monocytes, and T cells.27 In contrast to the bovine cathelicidins it is unknown whether the porcine cathelicidins are processed in the phagolysosome. Extracellular inhibition of elastase in wound fluids from pigs, which prevents activation of the porcine cathelicidins, impairs the clearance of bacteria from the wounds in vivo.58 Thus, cathelicidins seem to be important mediators of the extracellular antibacterial activity generated by neutrophils. In summary, we found that the human cathelicidin hCAP-18 is processed extracellularly to the antimicrobial peptide LL-37 by proteinase 3. This is the first detailed description of the generation of a human antimicrobial peptide from a promicrobicidal protein, and it demonstrates that the generation of active antimicrobial peptides from common proproteins occurs differently in related species.
We thank Hanne Kidmose, Allan Kastrup, Hans Janssen, and Nico Ong for their expert technical assistance. We thank Karsten Lollike, Jack B. Cowland, Kim Theilgaard-Mönch, Malene Bjerregaard, Daniel Carter, and Lene Udby for critical review of the manuscript, and we thank Veronique Witko-Sarsat for useful discussions.
Submitted September 20, 2000; accepted February 22, 2001.
Supported by grants from the Danish Medical Research Council and The Amalie Jørgensen Foundation.
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked "advertisement" in accordance with 18 U.S.C. section 1734.
Reprints: Ole E. Sørensen, Dept of Hematology, Granulocyte Research Laboratory, L-9322, Rigshospitalet, 9 Blegdamsvej, DK-2100 Copenhagen, Denmark; e-mail: olesoeren{at}rh.dk.
|
Top
Abstract
Introduction
for example,
bactericidal/permeability increasing protein (BPI),
