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RED CELLS
From the Departments of Biological Chemistry and of
Genetics, Institute of Life Sciences, Hebrew University, Jerusalem,
Israel.
The role of ferritin expression on the labile iron pool of cells
and its implications for the control of cell proliferation were
assessed. Antisense oligodeoxynucleotides were used as tools for
modulating the expression of heavy and light ferritin subunits of K562
cells. mRNA and protein levels of each subunit were markedly reduced by
2-day treatment with antisense probes against the respective subunit.
Although the combined action of antisense probes against both subunits
reduced their protein expression, antisense repression of one subunit
led to an increased protein expression of the other. Antisense
treatment led to a rise in the steady-state labile iron pool, a rise in
the production of reactive oxygen species after pro-oxidative
challenges and in protein oxidation, and the down-regulation of
transferrin receptors. When compared to the repression of individual subunits, co-repression of each subunit evoked a more than additive increase in the labile iron pool and the extent of protein oxidation. These treatments had no detectable effects on the long-term growth of
cells. However, repression of ferritin synthesis facilitated the
renewal of growth and the proliferation of cells pre-arrested at the
G1/S phase. Renewed cell growth was significantly less dependent on external iron supply when ferritin synthesis was repressed
and its degradation inhibited by lysosomal antiproteases. This study
provides experimental evidence that links the effect of ferritin
repression on growth stimulation to the expansion of the labile iron pool.
(Blood. 2001;97:2863-2871) The labile iron pool (LIP)1,2 of
mammalian cells has been implicated in cell iron regulation and in the
production of intracellular reactive oxygen species
(ROS).3,4 A major role in the intracellular modulation of
that pool has been attributed to ferritin (FT), particularly to its
heavy subunits (H-FT).5,6 This view is supported by
results obtained with cells overexpressing H-FT subunits in an
iron-independent manner.3,4 In accordance with the role of
FT as an iron scavenger, those cells displayed reduced steady-state
levels of LIP,4 reduced ROS production, and increased
resistance to ROS toxicity.3,4 Similar results were
obtained with HeLa cells transfected with the wild-type H-FT cDNA
linked to an inducible promoter.7
Although H-FT evoked a reduction in LIP and thereby affected related
cellular properties, growth and proliferation were modified only at
very high levels of H-FT expression.3,7 These and other
studies based on H-FT-transfected cells raise some questions about the
significance of strategies based on FT overexpression for understanding
FT involvement in the control of cell growth. On the one hand, the
transcribed FT message might be only partially translated, and most of
the resultant polypeptides might even lack the requisite functional
properties.8,9 On the other hand, cellular compensatory
properties might mask putative FT functions.3 Moreover,
short-term effects of FT expression might not necessarily be reflected
in the long term, and vice versa. In that respect, recent
studies10-13 suggested a link between the repression of
H-FT expression and the promotion of cell proliferation by oncogenes
such as c-myc14,15 and
E1A.10-13,16 However, earlier studies with
c-myc showed the opposite, namely that stimulation of growth
correlated with H-FT expression.17
To resolve the cellular roles of FT in iron metabolism and growth
through the modulation of LIP, we opted for a complementary approach to
that of gene overexpression. Antisense (AS) technology, which is based
on Watson-Crick hybridization with unique target mRNAs, was used as a
means to selectively suppress FT expression. AS oligodeoxynucleotides
(ODNs) were the tools used because they have already proven effective
in repressing gene expression in various erythroid and myeloid
cells18,19 and because, in iron-overexposed cells, they
were effective in repressing H-FT expression.20 The
FT-AS-ODNs were used here to specifically manipulate either (or both)
FT subunits in a gradual manner and independently of the iron status of
the cells. The AS-mediated repression of FT was analyzed at both the
message and the protein levels, and the resultant changes in LIP, ROS
production, and protein oxidation were assessed in cells by
fluorescence detection of metals,2 oxidation of
carboxydihydrofluorescein (CDCF),21 and carbonyl protein
oxidation assay,22 respectively. Our results provide experimental evidence for the concept that FT repression induces an
expansion of LIP and of LIP-related properties. Those properties allow
a more expeditious resumption of cell growth after its arrest, in
support of the proposed role of FT repression in the modulation of
short-term cell growth.
Materials and cells
FT quantitation by ELISA
Assessment of RNA levels of H-FT, L-FT, and L7 by reverse transcription-polymerase chain reaction Total RNA from K562 cells was isolated using Ultraspec RNA isolation reagent (Biotec Laboratories, Houston, TX) after cells were subjected to different FT AS-ODN treatments for 48 hours. This RNA (1 µg) was reverse-transcribed and polymerase chain reaction (PCR)-amplified using the Titan One Step reverse transcription (RT)-PCR system (Roche Diagnostics, Mannheim, Germany) according to the manufacturer's guidelines. In brief, total RNA was added to the PCR mix (which included the AMV reverse transcriptase, the Taq and Pwo DNA polymerases, and 40 U/µL RNase Inhibitor), together with the following primer pair for human H-FT (accession L20941): forward, 5'-GCCAAATACTTTCTTCACC-3'; reverse, 5'-TTCATTATCACTGTCTCCC-3'. These primers spanned a 390-bp sequence between residues 365 and 754. For human L-FT (accession M10119), the primer pair was: forward, 5'-AGGACATCAAGAAGCCAG-3'; reverse, 5'-AGGAAGTCACAGAGATGGG-3'. These primers spanned a 151-bp sequence between residues 318 and 468. For human L7 (an internal control, accession #X57958), the primer pair was: forward, 5'-GAAGAGAAGAAGAAGGAGG-3'; reverse, 5'-GGTACATAGAAGTTGCCAG-3'. These primers spanned a 239-bp sequence between residues 50 and 288. After reverse transcription at 50°C for 30 minutes (which was terminated by heating for 2 minutes at 94°C), touch down (TD) PCR was performed for 10 cycles using the following temperatures: denaturation (1 minute) at 94°C, annealing (2 minutes) at 65°C with a lowering increment of 2°C per cycle, and elongation (3 minutes) at 68°C. Then thermocycling was continued for up to 21 cycles under the following conditions: denaturation for 30 seconds at 94°C, annealing for 30 seconds at 45°C, and elongation for 45 seconds (+5 seconds per cycle) at 68°C. Samples were collected at cycles 16 to 21 after the TD-PCR. All RT-PCR reactions were performed using the Mastercycler thermocycler (Eppendorf, Hamburg, Germany). The samples were separated on a 1.6% agarose gel containing the SYBR-gold nucleic acid stain (Molecular Probes, Eugene, OR) and photographed under ultraviolet illumination. Optical densities of samples taken from cycles 16 to 21 were within a linear range.Immunofluorescence Cells fixed in methanol and treated with 3% BSA were incubated first with mouse monoclonal antihuman H-FT and subsequently with fluorescein isothiocyanate (FITC)-conjugated antimouse IgG and 1 µg/mL 4'-6'-diamidino-2-phenylindole (DAPI). Samples of 10 µL were loaded on a glass slide and examined in the Olympus IX70 (Tokyo, Japan) fluorescence microscope (exc 490 nm, em 520 nm). Images were processed using the EPIX (Buffalo Grove, IL) XCAP Image Acquisition, Display, Processing and Analysis software.LIP measurement and cell ROS production LIP was measured by the calcein-based fluorescence method, as described in detail previously.2 ROS was determined in cells (106/mL) loaded with 10 µM CDCDHF-AM (6'-carboxy-2',7'-dichlorodihydrofluorescein-acetomethoxy) for 20' at 37°C.21 The loading medium consisted of Dulbecco minimum essential medium (DMEM) supplemented with 20 mM Na-HEPES and 1% BSA. The reaction was stopped by rapid centrifugation and resuspension in HBS supplemented with hydroxyethyl-starch-DFO (Biomedical Frontiers, MN) to prevent extracellular metal catalyzed oxidation of the substrate, which might leak from the cells. The initial rate of H2O2 (5 µM)-induced conversion of the nonfluorescent 2'-7' CDCF to the 2'-7' oCDCF was measured fluorometrically (PTI, South Brunswick, NJ) (exc 488-517 nm). Maximum fluorescence (Fmax) was attained by permeabilizing cells with 0.5% Triton X-100 and exposing them to fresh 1 mM ferrous ammonium sulfate and 1 mM H2O2. ROS formation rate was assessed by the amount of oCDCF produced per second. oCDCF concentration in the cuvette at any given time was given by (oCDCF)c = (F/Fmax) × (oCDCF)cmax, where F and Fmax were the fluorescence at any given time and the maximal fluorescence, respectively, (both in relative units) and (oCDCF)cmax was the factor obtained by calibration with standard oCDCF. The cellular concentration of oCDCF, (oCDCF)in, was calculated by (oCDCF)in = (oCDCF)c/(NC*VC), where NC was the cell concentration in the cuvette (cells/mL) and VC was the volume of a single cell (ml/cell). Cells were counted in a cell counter, and the cell volume was obtained as described elsewhere.23Cell iron content Total cell iron was measured in triplicate samples of 5 × 106 cells suspended in 1 mL HBS buffer (150 mM NaCl, 20 mM HEPES). The cells were mixed with 1 mL acid mixture (3 N HCl, 10% trichloroacetic acid, and 3% thioglycolic acid) and incubated for 2 hours at 37°C, cooled, centrifuged at 3000 rpm for 30', and mixed with 0.5 mL bathophenanthroline sulfonate (0.045% in 4.5 N Na-acetate, 0.2% thioglycolic acid). Light absorbance of the solution was then read at 535 nm in a Spectronic 3000 UV-VIS Photodiode Spectrophotometer (Milton Roy, Oostende, Belgium).Tf-55Fe uptake and TfR expression K562 cells treated with FT-AS-ODN and resuspended (106/mL) in DMEM supplemented with 20 mM HEPES and 1 mg/mL BSA were exposed to an oversaturating concentration (15 µg/mL) of Tf-55Fe alone (total) or with 1 mg/mL Tf-Fe (nonspecific). Cells were subsequently incubated at 37°C in 5% CO2 and after 0, 30, 60, and 120 minutes were placed on ice for stopping the uptake. After 2 washes with cold PBS, 20-minute stripping with 1 mg/mL pronase E, 2 more washes with cold PBS, and resuspension in 200 µL PBS, triplicate 50 µL samples from each time point were counted for radioactivity in an aqueous Quicksafe A (Zinsser, Maidenhead, United Kingdom) scintillation fluid in a Beta counter (Beckman, Irvine, CA). Samples were also assayed for protein by the BCA method. Specific Tf-55Fe uptake was determined by subtracting the nonspecific counts from the total counts for each time point. The time 0 point, obtained by incubation at 4°C (no stripping), represented the saturating cell surface Tf binding, or TfR level.Protein oxidation Oxidative modification of proteins was assessed in cytosolic extracts by the carbonyl assay.22 Cells were extracted with 50 mM phosphate buffer, pH 7.4, containing 0.1% digitonin, antiprotease cocktail (see above), and 1 mM EDTA. Cells were subsequently centrifuged at 8500g for 2 minutes at 4°C to remove the nuclei. This was confirmed by obtaining a 260/280 absorption ratio of less than 1, and allowed omission of the streptomycin step for the removal of contaminating nucleic acids.Cell growth Viable cells were counted in a Coulter Cell Counter (Coulter Electronics, Harpended, Herts, United Kingdom), and their metabolic activity was measured by the Alamar Blue method according to the manufacturer's instructions. In brief, cells were plated in quadruplicate in 96-well culture plates at a density of 50 000/mL. Alamar Blue (5% final) was added at 24-hour intervals, and fluorescence (540-590 nm) was read in a BMG Fluostar plate reader after 4 hours of incubation in culture conditions. For the subtraction of background fluorescence, we used metabolically inhibited cells (ie, cells grown in the presence of 5 µM cycloheximide). Subtracted fluorescence values were normalized to those of untreated cells. Cell death index was determined on 10 000 cells by flow cytometry (Facscalibur; BDIS, San Jose, CA) with and without 5 µg/mL propidium iodide. The percentage of propidium iodide-positive (dead) cells was calculated from the 2 distribution curves.Cell-cycle progression after growth arrest K562 cells (initially at 200 000/mL), treated with different FT AS-ODNs for 24 hours, were supplemented with hydroxyurea (2 mM) for an additional 24-hour incubation. Cells were released from growth arrest after 2 washes with PBS and resuspension in growth medium. The distribution of 10 000 cells in the different cell-cycle phases (at 3, 6, and 9 hours) was obtained by flow cytometry as described above. For this analysis, cells were fixed in 75% methanol (1 hour at 20°C),
washed, and treated with propidium iodide (5 µg/mL). For assessing
the dependence of growth renewal on external iron, K562 cells treated
with AS-ODN for 24 hours were supplemented with 20 µM lovastatin for
24 hours, washed, and transferred to serum-free media containing
different concentrations (0-50 µg/mL) of Tf-Fe. The medium optionally
contained 4 mM mevalonic acid (a lovastatin inhibitor) and 20 µg/mL
leupeptin (a lysosomal protease inhibitor), which were renewed every 3 hours. At 3, 6, and 10 hours, they were analyzed for cell-cycle
distribution. The percentage of cells in the S phase at each time point
was obtained by ModFit LT Cell Cycle Analysis software (Verity,
Topsham, ME).
Repression of FT subunits Sequences of the human FT mRNA (H and L subunits), approximately 20 bp long, were screened by the software package GCG to trace for potential AS-ODNs. The main criteria for sequence exclusion were nonspecific homology to mRNA sequences other than hours and L-FT, self-annealing, repeat annealing, and high GC content. We selected 3 evenly spaced AS-ODNs in which the phosphodiester bonds of the 3 3' terminal bases were replaced with phosphorothioate bonds to minimize degradation by intracellular nucleases. Of these, a 20-bp long sequence complementary to a region in the center of the H-FT transcript was found empirically to maximally repress H-FT expression, and it was chosen for subsequent assays. This sequence (ASH-ODN) was 5'-CCAAATGTAATGCACACTCC-3'. The AS sequence for L-FT (ASL-ODN), 5'-GAGAGAGGTAGGTGTAGGAG-3', was obtained in a similar manner. One of the H-FT AS-ODN (5'-GAGATGCGGAGGATGCAAAT-3') was built in reverse order (IN-ODN) and used as a negative control in all experiments. Desferrioxamine treatment (DFO; 50 µM for 24 hours) was used as a positive control for the repression of expression of both FT subunits.24 AS-ODNs (1 nM-1 µM) were applied to cells (2 × 105/mL) in full medium with or without daily replenishment for 24, 48, and 72 hours. The effect of AS-ODNs on hours and L-FT levels in K562 cells is shown in Table 1. The most effective AS-ODN treatments against either H-FT or L-FT were found empirically to be after 48-hour incubation with 1 nM AS-ODN of the respective probes. Under these conditions, H-FT and L-FT levels were lowered to approximately 25% to 50% of the levels found in cells incubated with the reversed or inverted sequence (IN-ODN) of the AS-ODN, comparable to those attained with 50 µM DFO for 24 hours.
Blockage of FT expression by AS-ODN was also assessed at the message
level. As shown in Figure 1, the
FT-AS-ODN treatments reduced the L7-normalized mRNA level of H-FT
(ASH-ODN) to 60% of untreated cells and that of L-FT (ASL-ODN) to 77%
of untreated cells. The combined AS-ODN treatments (ASH-ODN + ASL-ODN) lowered the mRNA levels of both subunits, whereas the AS-ODN
treatments against one FT subunit did not affect the message level of
the other. The IN-ODN treatment exerted no significant effect on the FT
mRNA levels compared to the untreated cells. After these results, we
tentatively attributed the mode of repression to the reduction in the
respective mRNA levels.
Although repression of FT was most pronounced at the protein level
(Table 1), it was incomplete. To address whether this fact was
associated with cells being affected differentially by the AS-ODNs, we
analyzed them by immunofluorescence staining of FT with FITC-labeled
antibodies and of the cell nuclei with DAPI. We observed that the
expression of both FT subunits was abolished only in approximately two
thirds of the population of AS-ODN-treated cells (Figure
2). It remains to be established whether
the pattern of the AS-ODN effect reflects heterogeneity of transport of
the ODN into cells at different stages of growth or properties
associated with AS-ODN interaction with mRNA.
LIP, ROS, and TfR modulation by FT repression The effects of time and L-FT repression by AS-ODN on LIP and ROS formation were assessed in parallel. We postulated that by specifically decreasing hours and L-FT levels, the AS-ODN should evoke a sustained increase in steady-state LIP and ensuing metal-catalyzed ROS formation. This action of the AS-ODN is essentially different from a direct action on the LIP by chelating probes that is transient because of the activation of iron regulatory proteins (IRPs). Steady-state LIP, measured 48 hours after AS-ODN administration by a fluorescence method,2 was indeed significantly higher in the AS-ODN-treated cells than in untreated cells or cells treated with IN-ODN (Table 1, Figure 3A). An analogous rise in ROS production was observed when the AS-ODN-treated cells were challenged with H2O2 (5 µM) (Table 1, Figure 3B). This result indicates that the up-modulation of LIP by H-FT and L-FT AS-ODN treatment is associated with a catalytic (labile) reservoir. AS-ODN effects on all cellular parameters (including the LIP) were maximal within 48 hours. Thereafter the effects gradually disappeared even if the AS-ODNs were replenished (data not shown), thus indicating the occurrence of possible intracellular compensatory changes. Although the LIP levels in cells with various FT levels were different, their total iron content was apparently similar (Table 1). It is important to state, in that context, that the LIP is only a minor fraction (less than 1%) of the total cell iron (data not shown and 3).
Earlier studies showed that overexpression of an IRE-mutated H-FT
transgene repressed the levels of L-FT, possibly through a reduction of
the cellular LIP.25 In this work we applied the inverse
approach, based on AS-ODN tools, for a specific repression of each FT
subunit. It was therefore interesting to assess whether the repression
of one subunit might lead to an increased expression of its counterpart
by an expansion of the LIP. The results shown in Table 1 indicate a
compensatory increase in each FT subunit when its counterpart is
repressed. We tested whether this increase was mediated by the
increased LIP (Table 1). The results presented in Figure
4 indicate that the compensatory increase
in L-FT, observed when ASH-ODN was administered to the cells, was
reversible with the addition of the iron chelator DFO. This effect of
DFO was concentration dependent. DFO reversed the ASH-ODN-mediated
up-regulation of L-FT by scavenging iron because the addition of
iron-bound DFO could not revert L-FT up-regulation in H-FT-repressed
cells. These results support an active role of each FT subunit in
modulating LIP, with each balancing the another when one of them was
repressed. To overcome this compensation, we used AS-ODN treatment
against both FT subunits. The double AS-ODN treatment down-regulated
the expression of both FT subunits (Table 1), suggesting it overcame the compensatory increase is observed in one FT subunit when the other
was repressed (Table 1).
The double AS-ODN treatment brought forth an apparently synergistic increase in LIP compared to the sum of the AS effects on each FT subunit separately (Table 1). This can probably be attributed to the double AS treatment overcoming the compensatory increase in the nonrepressed subunit, which may restrain LIP up-regulation when only one FT subunit is repressed. However, the effect of the double AS-ODN treatment on ROS production was only partially additive, possibly indicating a saturating property. We have established that the repression of FT expression by AS-ODN probes can lead to an increased steady-state LIP, and we have ascertained that this iron pool is indeed reactive (Table 1, Figure 4). To corroborate the latter, we also examined the effects of metal-stimulated oxidation of proteins, which is assumed to proceed through free radical attack. We found that only FT double AS-ODN treatment led to a significant rise in protein oxidation, comparable to the rise obtained on incubating the cells with 50 µM ferric ammonium citrate (data not shown), which is known to substantially increase the LIP26 (Table 1). The up-modulation of LIP by repression of either or of both FT subunits
was concomitant with a decrease in both TfR-mediated iron uptake
(Figure 5A) and surface expression
(Figure 5B). This observation indicates that the increased LIP formed
by FT repression is also reactive inasmuch as the reduced TfR
expression is probably related to IRP inactivation.27,28
Despite the large differences in the steady-state LIP levels evoked by
the double FT-AS-ODN and the single FT-AS-ODN treatments (Table 1),
their effects on Tf-Fe uptake and TfR expression were similar (Figure
5). This and the fact that addition of iron salts (20 µM for 24 hours) had similar effects on Tf-Fe uptake and TfR expression because of the different FT-AS-ODN treatments (Figure 5) point to a ceiling effect of LIP on TfR modulation.
Growth modulation by FT repression and iron chelation The effects of FT repression by AS-ODN treatment of cells were assessed in cells grown in normal culture conditions and following stimulation after growth arrest. Viable cell count (Figure 6A), gross metabolic activity (Figure 6B), and cell death index (Figure 6C) were found to be similar in AS-ODN-treated cells and in control cells when observed for a period of 6 days. The same results were obtained regardless of whether the AS-ODN probes were replenished daily (data not shown). On the other hand, when the cells were growth arrested at G1/S, FT AS-ODN treatment had a prominent effect on recovery from arrest. This was manifested in the DNA content of cells released from hydroxyurea-mediated cell-cycle arrest at G1/S (Figure 7): 3 to 9 hours after the release from the blockage, 50% to 60% of the cells entered the S phase when treated with AS-ODN against H-FT+L-FT compared with 40% to 45% when treated with none or with IN-ODN (Figure 7A). To corroborate that the above results truly and significantly reflected entry of cells into the S phase, we also assessed the cell DNA content 24 hours after lifting the cell-cycle blockage. A positive correlation was found between the percentage of cells in G2/M and the percentge of cells in the S phase 15 to 21 hours earlier (Figure 7A-B).
To assess the role of iron in the cell cycle properties affected by FT
repression, we examined the effects of DFO on the entry of cells into S
phase after their release from growth arrest. Iron chelator treatment
led to a general repression of growth as reflected in the lower
percentage of cells in the S phase. (Although the percentage of
DFO-treated cells at the S stage remained below control levels up to 9 hours after the blockage was lifted [Figure 7A], growth resumption
was relatively faster. We attributed the latter to increased expression
of TfR induced by iron deprivation.52-54) This
was also found when the initial treatment of cells included FT-AS-ODN
(Figure 7A). Based on the specificity of DFO for iron chelation, we can
infer that the increase in cell iron availability evoked by AS
repression of FT (Table 1, Figure 3A) contributed to a faster
resumption of DNA synthesis. Two major physiological mechanisms furnish
iron to the LIP, membrane iron uptake systems,1 and iron
release from FT, primarily by protein degradation.29-31 To
set up conditions whereby cells will be dependent only on external iron, we used leupeptin as a blocker of lysosomal proteases and the
ensuing release of iron from FT.30 For arresting cells at the G1/G0 phase, we used the cell-cycle blocker
lovastatin. (We chose lovastatin rather than hydroxyurea for its
ability to confine cell arrest to the G1 and not the
G1/S phase.55,56 Nonetheless, a small fraction
of the cell population is also arrested at the G2 phase
[Muller et al57 and data not shown]. The latter probably introduced some variability to the cell-stage distribution 24 hours
after cell-cycle arrest was lifted, thus preventing us from tracing
further cell-cycle progression, as shown earlier [Figure 7B].) We
surmised that because FT-AS-ODN (both hours and L) treatment up-modulates LIP, growth resumption should be less dependent on external iron in the FT-AS-ODN-treated cells than in the untreated ones. K562 cells were incubated in full medium in the presence of
FT-AS-ODN probes, and 24 hours later they were growth arrested at
G1 by 20 µM lovastatin. Release from lovastatin blockage
was achieved by adding 4 mM mevalonic acid. The cells were suspended in
an iron-free medium supplemented with Tf-Fe (various concentrations), and leupeptin was added periodically (every 3 hours) to maintain a
constant proteolytic blockage. As shown in Figure
8, cells arrested at G1
resumed growth relatively faster and at much lower Tf-Fe concentrations
when they were pretreated with hours and L-FT-AS-ODN. Inspection of
Figure 8C shows that, even without the addition of Tf-Fe, up to 39% of
the cells were already in the S phase by 10 hours after release from
lovastatin. Such an effect was equivalent to the one attained by the
addition of 50 µg/mL Tf-Fe to cells treated either with nothing
(Figure 8A) or with IN-ODN (Figure 8B). Moreover, exposing the cells
for 24 hours to relatively low concentrations of DFO (10 µM) in the
presence of FT-AS-ODN or to DFO alone at higher concentrations (50 µM) rendered their progression through the cell cycle more dependent
on Tf-Fe (Figure 8D-E, respectively). This was reflected in the
percentage of cells that reached the S phase after lovastatin release,
as shown in the time dependence of growth (Figure 8E) or in the Tf-Fe
concentrations required for half-maximal growth (Figure 8F). As shown
in Figure 8F, cells treated with AS-ODN against both hours and L-FT
subunits passed half-maximal percentage in S even without Tf-Fe
supplementation. We tentatively ascribe the increased dependence of the
growth rate on Tf-Fe concentration (Figure 8E) to increased TfR
expression induced by DFO depletion of the LIP.32 Taken
together, the results presented in Figure 8 strongly suggest that the
pool of iron affected by FT is rate limiting for the renewal of DNA
synthesis. FT-AS-ODN treatment, which expanded the LIP, allowed
intracellular-iron-depleted cells to bypass the need for importing
iron when growth arrest was lifted.
FT has been implicated in cell iron detoxification,33,34 protection against oxidative stress,5,6,35 and various inflammatory conditions.36,37 The major regulatory mechanism of FT expression has been associated with the inactivation of IRPs by an alleged rise in LIP. However, it has also been assumed that FT actively modulates the LIP by IRP-independent mechanisms acting at the transcriptional level.13,38,39 That form of FT gene repression has been thought to support oncogene-induced proliferation10-16 by purportedly increasing iron availability. In the current study we examined the hypothesis that attenuation of FT expression has implications for cell growth because of the alleged capacity of FT to down-modulate LIP levels.4,6 To ascertain that FT can actively reduce the LIP, FT expression was manipulated independently of the cellular LIP by using AS technology. Repression of FT gene expression involves AS-ODN hybridization with the target,18 leading either to blockage of translation or to target degradation by RNase H. The main drawbacks of this approach are nonspecific actions and potential toxicity of the AS-ODN probes40,41 and a possible feedback up-regulation of the target of suppression. The most effective and selective AS probes were obtained by screening the potency of AS-ODN against various sites along the sequences of either H-FT or L-FT, using the specific inhibition of only one FT subunit as the selection criterion. The effects obtained with nM concentrations of probes were optimal after 48-hour treatment (approximately 70% mean inhibition) (Table 1, Figure 1) but were apparently heterogeneous in the cell population (Figure 2). Qualitatively and quantitatively similar results were obtained by transient transfection with an FT-AS fragment (O.K. et al, unpublished observations, 2000). AS-ODN repression of FT synthesis shifted the cells into a state of relatively higher LIP (Table 1, Figure 3A), indicating that FT repression can override the LIP-mediated control of FT levels through IRPs.27 LIP increase attained by AS-ODN repression of each FT subunit was specific inasmuch as it was accompanied by an increased synthesis of the counterpart subunit (Table 1) and a decreased activity of TfR (Figure 5). The proposed involvement of LIP in the mode in which one FT subunit affects expression of the other25 was also supported by the fact that the inter-subunit effect was prevented by iron chelators (Figure 4). The possibility that the AS-ODN-mediated repression of FT was evoked by a potential chelating activity of the ODN42 can be rejected because (1) an ODN containing an identical composition of bases in the reverse order (IN-ODN) produced no repression of FT, (2) FT repression by the AS-ODN was subunit-specific, and (3) LIP was increased, rather than reduced, by the FT-AS-ODN action. Changes in LIP evoked by active down-modulation of FT were also reflected in increased ROS formation and in oxidative modification of cell proteins. This complements studies of up-modulation of FT expression that resulted in reduced LIP and ROS formation.3,4 Taken in toto, all these studies provide experimental support for the alleged "active" role of FT in regulating LIP and attenuating ROS generation. This work assessed the hypothesized role of FT as a cell growth modulator based on its capacity to modulate LIP levels. Although we found AS treatments against H-FT, L-FT, or both to substantially increase LIP levels, they failed to affect long-term cell growth, viability, and metabolic activity (Figure 6). In contrast, growth resumption of cells arrested at G1/S (Figure 7) or at G1 (Figure 8) was significantly faster when FT expression was inhibited and LIP was increased. Two lines of evidence support the hypothesis that FT contribution to the short-term cell growth was through increased LIP. First, AS effects were reversed with the addition of an iron chelator (Figures 7, 8). Second, growth was less dependent on iron import when cells were FT-repressed (Figure 8). Nonetheless, the differential effects of FT repression on stimulated growth vis-a-vis normal growth remain unexplained. In both forms of growth, iron can be a rate-limiting factor (Hann et al,43 Kovar and Franek,44 Kovar,45 Seligman et al,46 and O.K., unpublished observations, 2000), and FT might control cell growth by restricting cell iron availability.7 It is possible that a substantial expansion of LIP occurred only in a fraction of cells, those most affected by AS-ODNs (Figure 2), and that in the long term these effects are diluted out in the population. In the short term, however, such as in growth resumption after arrest or at a particular stage in the cell cycle, the demands for iron might be more acute and, thus, the AS effects more pronounced. This applies also to the G1 cyclins E/cdk2, D/cdk4,47,48 and D149 and the S cyclin A/cdk2,48 which are iron-dependent factors that contribute to cell cycle progression. In nonsynchronized cultures, LIP iron might not be rate limiting because those factors are maintained at steady levels and no accumulation of stage-specific, iron-dependent factors is anticipated. On the other hand, in synchronized cultures, an accumulation of such factors is apparently needed for cell cycle progression; thus, iron from the LIP might be essential. Clearly, asynchronous or unstimulated cell growth might differ from accelerated growth after the release from cycle arrest or from oncogene activation in their demands for labile iron. This is reflected by both oncogene down-modulation in iron deprivation50 and potentiation in iron supplementation.51 Our work is in support of the paradigm that stimulated cell growth is sustained by FT repression-mediated up-regulation of the LIP.
Submitted September 13, 2000; accepted December 19, 2000.
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked "advertisement" in accordance with 18 U.S.C. section 1734.
Reprints: Z. Ioav Cabantchik, Department of Biological Chemistry, Institute of Life Sciences, Hebrew University, Jerusalem 91904, Israel; e-mail: ioav{at}cc.huji.ac.il.
1.
Breuer W, Epsztejn S, Cabantchik ZI.
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Abstract
Introduction
-galactosidase (Amersham, United Kingdom) served as
secondary antibodies in conjunction with fluorescein-
.013 and P 
4, respectively). ASL-ODN and ASH-ODN+ASL-ODN
also significantly reduced the L-FT mRNA band densities (P 
) or IN-ODN (
). In both the
ASH-ODN (
) and the IN-ODN (
), 50 µM iron-bound DFO was also
supplemented. (Inset) Influence of 48-hour incubation with 1 nM ASH-ODN
on the expression of both H-FT (
) compared with the
influences of DFO (50 µM, 24 hours), IN-ODN (1 nM, 48 hours), and
none (48 hours). Protein levels were quantified by ELISA, as described
in "Materials and methods." Data are from a representative
experiment performed in triplicate.
), IN-ODN (
), untreated cells; (
