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HEMATOPOIESIS
From the Harvard Institutes of Medicine, Harvard
Medical School; and Dana Farber Cancer Institute; both of Boston, MA;
Department of Microbiology and Immunology, Indiana University School of
Medicine, Indianapolis, IN; and The Burnham Institute, La Jolla, CA.
The transcription factor PU.1 (also known as Spi-1) plays a
critical role in the development of the myeloid lineages, and myeloid
cells derived from PU.1 PU.1 is an Ets family transcription factor
that plays an essential role in the development of both myeloid
(granulocytes and monocytes/macrophages) and lymphoid
lineages.1-3 PU.1 regulates almost every myeloid gene
promoter studied to date, such as CD11b.4 PU.1 null mice
have an absolute block in the development of monocytes/macrophages and
B lymphoid cells. Granulocytes and T cells are also affected in that
there is a delay in their development as well as abnormalities in
neutrophil function.5,6 Subsequent analysis of
PU.1 Expression of PU.1 is restricted to hematopoietic lineages. PU.1 is
expressed in multipotential progenitors, including
erythroblasts.14 PU.1 expression is tightly regulated
during subsequent hematopoietic differentiation: PU.1 is up-regulated
with myeloid and down-regulated with erythroid
commitment.15 In mature hematopoietic cells, PU.1 is
expressed in myeloid and B lymphoid cells but not erythrocytes or T
cells.1,14,16,17
As a critical determinant of hematopoietic lineage development, it is
essential that PU.1 is properly regulated. PU.1 was originally isolated
as the gene product of the Spi-1 locus as a protooncogene identified as
a common spleen focus-forming virus integration site in murine
erythroleukemia cells.18,19 The spleen focus-forming virus
insertion leads to activation of PU.1 expression and blocks terminal
differentiation of erythroblasts.18,19 Furthermore,
overexpression of PU.1 in a transgenic model results in
erythroleukemia.20 During normal hematopoietic
development, PU.1 expression increases as stem cells commit to the
myeloid lineage,15 and PU.1 expression is turned off with
erythroid differentiation as GATA-1 expression
increases.15 The importance of proper regulation of PU.1
is demonstrated by several recent studies showing that PU.1 suppresses
GATA-1 activity to block erythroid differentiation.21-24
Previous promoter studies using transient transfections demonstrated
myeloid cell type-specific regulation confined to a 334-base pair
(bp) promoter sequence.25,26 Within this region,
functional binding sites for octamer binding factors, Sp1, and PU.1
were identified.25,26 We have previously shown that the
PU.1 promoter is autoregulated by PU.1 itself and that PU.1 in
conjunction with Sp1 is important for myeloid expression, while
regulation by octamer binding proteins in conjunction with the B
cell-specific coactivator Bob-1 is likely to direct expression in B
cells.25,26 A recent study demonstrated that C/EBP Therefore, we undertook additional experiments to ask how PU.1 is
regulated in vivo. Here we show that cell type- and tissue-specific chromatin-independent expression of the murine PU.1 gene can be conferred by a 91-kb genomic DNA fragment in both stable cell lines and
transgenic mice. Within this 91-kb PU.1 locus, 3 potential transcriptional regulatory elements were identified using DNase I
hypersensitive site (HS) studies. One of the 3 elements was shown
to greatly enhance basal promoter activity and is likely to
contain an important previously unidentified regulatory element.
Cell culture
Isolation of the murine PU.1 P1 clone and
plasmid constructions
Preparation of linearized DNA from P1 constructs for generation of transgenic mice and stable cell lines The mPU.1-Thy1.1-P1 plasmid DNA was purified using a Qiagen Midi Kit followed by phenol extraction and ethanol precipitation. The plasmid was linearized with MluI and purified on a Sepharose CL4b column (Pharmacia, Valencia, CA) as previously described.32 The same linearized mPU.1-Thy1.1-P1 fragment, which contained the P1 vector, was used for making either transgenic mice or stable cell lines.Transfections Transient transfections were performed as described previously.33 U937, 416B, and BW5147 cells were electroporated at 300, 270 and 250 V, respectively, and 960 microfarads. Cells were harvested approximately 5 hours after electroporation. Transfection efficiency was normalized with the activity of cotransfected Renilla luciferase activity.34 Individual transfections were repeated 3 times. For stable cell lines, plasmid DNA (except for the mPU.1-Thy1.1-P1 clone) was purified with the Qiagen Maxi DNA purification kit. Stable cell lines were generated by electroporation of 2 × 107 416B or BW5147 cells, respectively, and 1 × 107 U937 cells as described for the transient transfections. Plasmids used for stable lines included 5 µg NdeI-linearized pXP2/ 0.334 kb/Luc,
AatII-linearized pXP2/14 kb/0.334 kb/Luc,
SalI-linearized pPU.1/1.3 kb/Thy1.1, or
XhoI-linearized pPU.1/1.3 kb/Intron I/Thy1.1 cotransfected
with 0.2 µg NaeI-linearized pGK-puro.35 Cells
were plated on 96-well plates and selected for transfected stable
clones with 2 µg/mL puromycin 48 hours after electroporation.
Luciferase assays Luciferase assays were performed according to the manufacturer's (Promega) protocol. Cell lysates were quantitated with the Bradford protein assay (Bio-Rad Laboratories, Hercules, CA).Southern blot analysis Genomic DNA was separated on agarose gels, blotted onto BioTrans+ membranes, hybridized, and washed as described.36 Stable chromosomal integration of the murine Thy1.1 or luciferase genes was detected by either a random-primed labeled 544-bp BamHI fragment of the murine Thy1.1 cDNA or a 1.7-kb XbaI fragment of the luciferase gene from pXP2.37Northern blot analysis Total RNA was isolated from stably transfected cell lines by guanidium extraction38 and from transgenic animals following tissue homogenization in 4 M guanidine isothiocyanate solution and separated on cushions of cesium trifluoroacetate (Amersham Pharmacia Biotech, Piscataway, NJ). Murine Thy1.1 RNA was detected with a labeled 544-bp BamHI fragment of the murine Thy1.1 cDNA,31 murine PU.1 messenger RNA (mRNA) with a 431-bp PU.1 5' cDNA fragment (which is specific for murine PU.1),17 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA with a 1.3- kb PstI fragment of rat GAPDH cDNA.39 Blots were subsequently hybridized for detection of Thy1, murine PU.1, and GAPDH mRNAs, respectively, and the blot exposed for several days in between hybridization to ensure that the previous probe had indeed been removed prior to subsequent hybridization.DNase I hypersensitive site studies DNase I hypersensitivity was assayed as described.36 Probes were as follows: probe 1 is a 1.4-kb EcoRI-PstI fragment located13 kb 5' of the
transcriptional start site; probe 2 is a 2-kb BamHI-EcoRI fragment including 1.9 kb of intron 1 and exon 2; probe 3 is a 1.5-kb BamHI-EcoRI
fragment from the 3' end of the gene (located 1 kb downstream from exon
5); probe 4 is a 1.1-kb EcoRI-PstI fragment
located 12 kb 5' of the transcriptional start site; probe 5 is a 657-bp
BamHI-DraIII fragment of intron 1; and probe 6 is
1.5 kb EcoRI-PstI fragment located 15 kb 5' of
the transcriptional start site.
Transgenic mice MluI-linearized mPU.1-Thy1.1-P1 purified as described above was resuspended in 1 mM Tris, pH 8.0, 0.01 mM ethylenediaminetetraacetic acid, injected into fertilized oocytes of FVB mice, and implanted in uteri of pseudopregnant FVB mice according to standard procedures.31Isolation of lineage-specific cell populations from transgenic animals B220+ and CD4+/CD8+ (meaning positive for either or both T-cell markers) cells were isolated from spleen and thymus, respectively, of PU.1 transgenic mice. Mac-1+ cells are isolated from peritoneal macrophages collected 48 hours after thioglycollate injection. To obtain Gr-1+ cells, 5 mg G-CSF was injected subcutaneously every 12 hours for 4 days prior to injection of thioglycollate. Granulocytes were collected from the peritoneal wash 4 hours later. This procedure yields high numbers of more than 90% granulocytic cells.17,40 Each cell population was incubated with rat anti-B220, anti-CD4 and anti-CD8, anti-Mac-1, or anti-Gr-1 antibody, respectively (Pharmingen). Subsequently, cells were incubated with goat antirat immunoglobulin G Microbeads and purified over a RS+ Separation Column and VarioMACS magnetic system (Miltenyi Biotec).RT-PCR analysis of transgene expression in purified Mac-1+ myeloid and TER119+ erythroid cells Mac-1+ cells and TER119+ cells were isolated from bone marrow cells of PU.1 transgenic mice. Bone marrow cells were stained with anti-Mac-1 antibody and anti-TER119 antibody (BD Pharmingen, San Diego, CA), and each 10 000 Mac-1+ cells and TER119+ cells were sorted using a highly modified triple laser (488 nm argon laser, 599 nm dye laser, and ultraviolet laser) Cytomation system (MoFlo, Fort Collins, CO). The purity of sorted cells was more than 99%. Total RNA was extracted with Tri-reagent (Molecular Research Center, Cincinnati, OH) with 10 µg yeast transfer RNA as a carrier. The cDNA was synthesized with Superscript II RNase H-Reverse Transcriptase (Gibco, Gaithersburg, MD). Reverse transcriptase-polymerase chain reaction (RT-PCR) was performed with RNA equivalent to 200 cells each and Pfx DNA polymerase (Gibco). Primers for transgene-derived mRNA were PU.1 5' sense primer 5'-AAACCTTGTCCCCAGCCCACCAG-3' and Thy1.1 3' antisense primer 5'-GCAGTCCAGGCGAAGGTTTTG-3'. Primers for murine GATA-1 were exon 2 5'-GCATCAGCACTGGCCTACTACAG-3' and exon 3 5'-CCGTAAGCACTGCCGGTGACAGG-3'.41 Primers for murine GAPDH were 5'-GGTGCTGAGTATGTCGTGGAGTCTA-3' and 5'-CAAAGTTGTCATGGATGACC-3'.Western blot analysis of transgenic PU.1 proteins Cell lysates were extracted with RIPA buffer. Proteins were detected with a PU.1 antibody recognizing an N-terminal PU.1 peptide,4 a PU.1 antibody that recognizes a C-terminal PU.1 peptide (Santa Cruz), and an anti-Thy 1.1 antibody (Santa Cruz).
PU.1 promoter fragments that confer high-level activity in transient transfections do not express in transgenic mice Previously published transient transfection studies had established that a 500-bp BamHI fragment containing 334 bp of the murine PU.1 promoter and 166 bp of 5' untranslated region ("0.5-kb promoter"), as well as a 2.1-kb SacI fragment containing this promoter and additional 5' sequences ("2.1-kb promoter"), conferred high-level promoter activity in myeloid and B-cell lines but not in other cell types.25,26 We asked whether either of these 2 constructs could direct expression in transgenic mice. We analyzed 4 founder lines with the 2.1 kb and 5 lines with the 0.5-kb promoter using a -galactosidase
reporter,31 and in none of the lines did we observe
significant expression (detectable by -gal staining) in B cells, T
cells, or peritoneal macrophages (data not shown). Because we were
concerned that the -gal reporter system we were using might not work
efficiently in granulocytes, we remade the transgenic constructs with
the murine Thy-1 reporter gene that we had used successfully to measure
CD11b promoter activity in transgenic mice both by
fluorescence-activated cell sorter (FACS) and Northern blot
analysis.31 In 12 independent transgenic founder lines 6
with the 2.1-kb and 6 with the 500-bp PU.1 promoterwe observed no
detectable Thy1.1 surface expression by FACS of peripheral blood,
spleen (source of B cells), peritoneal macrophages, or thymus (source
of T cells) or mRNA by Northern blot of peritoneal macrophages, spleen,
or thymus (data not shown). In summary, these experiments indicated
that elements in addition to the promoter are important for expression
in transgenic mice.
A murine PU.1 91-kb genomic fragment confers high-level reporter gene expression in myeloid cell lines To direct cell type-specific gene expression in a chromatin-independent manner, additional regulatory elements must be needed. Therefore, we isolated a 91-kb murine genomic P1 clone containing the murine PU.1 gene (Figure 1). Using a combination of field inversion and traditional agarose gel electrophoresis, we established a restriction map of the clone (Figure 1). The 20-kb murine PU.1 gene is located almost in the center of the P1 clone, with approximately 35 kb of 5' and 3' flanking sequence. A few hundred base pairs from each end of the 91-kb PU.1 genomic fragment were sequenced. The 5' end sequence demonstrated identity with the 3' end of murine tat binding protein-1 (GenBank accession No. D49686), while the 3' end sequence of the PU.1 fragment did not demonstrate similarity to any known sequences (data not shown).
We next used homologous recombination in bacteria32 to insert a Thy1.1 cDNA as a reporter into the PU.1 locus. We selected Thy1.1 because we have experience using it as a sensitive and effective reporter of protein and mRNA expression in macrophages and neutrophils of transgenic mice.31 We successfully inserted the internal ribosome entry site (IRES)-Thy1.1 construct into the PU.1 P1 clone, replacing part of exons 4 and 5. The targeted P1 clone (mPU.1-Thy1.1-P1) would be expected to express Thy1.1 mRNA from the PU.1 transcriptional unit with a predicted 3-kb mRNA species. We would also predict that the clone would efficiently translate Thy1.1 protein from the IRES but that the PU.1 coding region would be interrupted and a functional PU.1 protein not expressed. We generated 9 independent stable lines of human U937 myeloid cells
with the targeted mPU.1-Thy1.1-P1 clone. No deletions or rearrangements
were noted following separation of restriction fragments by field
inversion gel electrophoresis (data not shown). Thy1.1 mRNA was
expressed in every clone harboring the transgene in both U937 (Figure
2) and 416B (data not shown) stable cell lines. No Thy1.1 mRNA was detected in parental U937 cells (Figure 2A,
lane 11) or clone 14 (Figure 2A, lane 5), which does not contain the
transgene (data not shown). The T-cell line BW5147, which served as a
positive control for Thy1 expression, did indeed express Thy1.1 mRNA
but with a more slowly migrating species (1.85 kb vs the major band of
1.4 kb observed in the U937 transfectants). We observed 3 major mRNA
species, which hybridized to the murine Thy1.1 probe in the
transfectants of 3 kb, 2 kb, and 1.4 kb. The same pattern was
consistently observed in every stable cell line as well as in
transgenic mice in vivo (see below); therefore, the multiple RNA
species observed are independent of the integration site and more
likely an inherent property of the transgene. As noted above, we
predicted a predominant 3-kb mRNA species but, in addition, observed a
2-kb species resulting from a cryptic splicing event (see below) as
well as a predominant 1.4-kb mRNA, which presumably arises from yet
another cryptic splice donor or acceptor we have not yet mapped.
We performed Southern blot analysis to determine the number of copies of the transgene in each stable line (data not shown). In general, expression of Thy1.1 mRNA correlated very well with copy number. Therefore, it appears that the transgene is expressed in a copy number-dependent manner, although more clones with intermediate transgene copy numbers would be required to state this with certainty. Initially, we analyzed reporter Thy1.1 protein expression by FACS analysis, but no detectable Thy1.1 surface expression was observed in any of the expressing clones (data not shown). We therefore performed further RT-PCR and sequencing analysis using total RNA isolated from the highest Thy1.1 mRNA expressing clone (No. 16). Sequencing of the RT-PCR product derived from this clone demonstrated that the IRES sequence intended to provide with ribosomal binding sites for efficient translation initiation had been spliced out using a cryptic splice donor found in murine PU.1 exon 4 (nucleotide 555, in the middle of exon 4) and splice acceptor in the middle of the murine Thy1.1 cDNA (Figure 2B). DNase I hypersensitivity assays detect 3 sites within the murine PU.1 locus We next sought to localize the important regulatory element(s) within the 91-kb murine PU.1 fragment that conferred expression in cell lines. Transcriptionally active regions in chromatin have an open configuration that allows transcription factors to bind to and are therefore more susceptible to DNase I digestion. To define the regulatory elements in the 91-kb PU.1 genomic DNA fragment, a 50-kb region spanning from20 kb upstream to 10 kb downstream of the PU.1
gene was systematically searched for DNase I HSs (Figure 3). Three DNase I HS regions were
observed (Figure 3) as shown by Southern analysis of DNase I-treated
genomic DNA. The first hypersensitive region (Figures 1B and 3A) is
located 14- kb upstream of the transcriptional start site. This 14-kb
HS appears not to be myeloid specific, because it was observed in both
myeloid and the T-cell line BW5147, which does not express PU.1 (Figure 3A). The second hypersensitive region mapped to the proximal promoter and intron 1 consisted of at least 3 distinct sites observed in 416B
murine myeloid cells but not in BW5147 T cells. The +0.149 kb HS was
localized near the proximal promoter, while the other 2 localized to intron 1 (Figure 3B). The third hypersensitive region was located in intron 2 with +9 kb, +12 kb, and +15 kb HSs
observed only in 416B myeloid cells, while the +17 kb site was observed
in both 416B myeloid and BW5147 T cells (Figure 3C). We did not observe
specific DNase I HSs with probes 4, 5, and 6.
The 14-kb DNase I HS had a positive regulatory
effect on the minimal promoter activity, a 3.5-kb HindIII fragment containing this site was subcloned in both orientations in
front of the 334 PU.1 promoter directing luciferase reporter gene
expression along with appropriate control plasmids (Figure 4A). To ask whether this 3.5-kb fragment
could function as a classical enhancer, these constructs were
transiently transfected into either myeloid (U937 and 416B) or T-cell
(BW5147) lines. As shown in Figure 4A, the 334-bp PU.1 promoter by
itself demonstrated relatively high levels of luciferase activity in
U937 cells, which express relatively high levels of
PU.1.17 Lesser activity was observed in 416B cells, which
represent a multipotential line and express lower levels of endogenous
PU.1 mRNA (H.S.R. and D.G.T., unpublished results, 2001), and
lesser still in BW5147 T cells, which do not express detectable levels
of PU.1 (Figure 4A). When the 3.5-kb BamHI fragment
containing the 14-kb HS was placed in either the genomic or reverse
genomic orientation, no significant augmentation of either activity or
specificity was observed compared with the 334-bp minimal promoter in
transient transfections. Therefore, in the assay used, the 14-kb HS
does not act as a classical enhancer element.
However, we obtained a significantly different result when the same
The murine PU.1/91-kb genomic fragment confers tissue-specific reporter gene expression in transgenic mice The results from analysis of the expression of the PU.1 transgene in cell lines suggested that this clone, in contrast to the smaller 2.1-kb promoter construct, contained sufficient regulatory sequences to direct expression in vivo. To test this, we established transgenic mouse lines containing this P1 clone. To date we have been able to establish lines from 2 independent founders that harbored approximately 6 copies of the transgene. In these 2 lines, high-level reporter gene mRNA expression was detected using a Thy1.1 probe in RNA derived from peritoneal macrophages, bone marrow, and spleen from transgenic animals (Figure 5A), with the same multiple RNA species as observed in stable cell lines (Figure 2A). No Thy1.1 mRNA was detectable in any nontransgenic animal tissue except for in the thymus, in which both the transgenic and nontransgenic littermates gave the same pattern, which is likely a result of endogenous Thy1.1 expression. After very long exposures, weak Thy1.1 hybridization signals could be detected in other tissues of transgenic animals, most likely arising from the presence of macrophages or granulocytes in these tissues (data not shown). Using a murine PU.1 probe, we observed a pattern of expression similar to that of the Thy1.1 probe in peritoneal macrophages, bone marrow, and spleen in transgenic animals but only a single strong mRNA species consistent with the endogenous PU.1 RNA17 in nontransgenic tissues and very weak hybridization in the thymus. These results indicate that the transgene expresses an mRNA species hybridizing to both the Thy1.1 and the PU.1 probe in tissues in which endogenous PU.1 is strongly expressed (peritoneal macrophages, bone marrow, spleen) but not in which PU.1 is weakly expressed (heart, kidney, lung, thymus). Both founder lines (including the one depicted in Figure 5A) have approximately 6 copies of the PU.1 transgene (data not shown). The mRNA expression levels are 3-fold and 3.5-fold of that of endogenous PU.1 by Northern blot analysis; therefore, we estimate that the amount of mRNA per transgene is very similar to that of the endogenous murine PU.1 gene. However, the number of founder lines is too small to draw precise conclusions as to whether expression correlates with transgene copy number. We predicted that no significant amount of PU.1-Thy1.1 hybrid protein would be produced because both an efficient ribosome binding site and start ATG codon were missing from the mRNA produced by the transgene (Figure 2B). By Western blot analysis using amino-terminal- and carboxyl-terminal-specific anti-PU.1 and anti-Thy1 antibodies, we were unable to detect such a PU.1-Thy1 fusion protein in transgenic mice expressing this transgene (data not shown). Therefore, it is very unlikely that a fusion peptide encoded by the transgene affects its expression.
To determine which bone marrow cells expressed PU.1 mRNA, we fractionated bone marrow cells into granulocytes, monocytes, B cells, and T cells. As expected, the Thy1.1 probe hybridized to a single strong RNA species in CD4+ and CD8+ T cells, consistent with hybridization to the endogenous Thy1.1 mRNA (Figure 5B). In addition, the Thy1.1 probe detected 3 RNA species in granulocytes, monocytes, and B cells that do not normally express it, in a pattern similar to that observed in cell lines (Figure 2A) and mice (Figure 5A) harboring the P1 transgene. The expression pattern of Thy1.1 mRNA in these cells (granulocytes, monocytes, and B cells but not in T cells) is similar to that detected with the PU.1 probe (Figure 5B). By RT-PCR (Figure 5C), we could easily detect transgene RNA expression in Mac-1+ myeloid cells, but we were unable to detect any evidence for transgene RNA in TER119+ erythroid cells. In summary, these results, combined with those obtained in stable cell lines, suggest that all of the regulatory elements necessary for high-level, cell type-specific PU.1 expression are contained within this 91-kb fragment.
Our laboratory and others demonstrated that as little as 334 bp of murine PU.1 promoter sequence confers cell type-specific gene expression in transient transfection assays.3,25,26 However, this promoter, as well as a longer fragment extending up to 2.1 kb, could not direct detectable reporter gene expression in multiple lines of transgenic mice, suggesting that additional transcriptional regulatory elements are needed to function in the context of chromatin. Because studies from other laboratories have demonstrated that elements that direct high-level expression in transgenic animals are often located tens of kilobases from the promoter,36,42-46 we expanded our search for these elements. We isolated a 91-kb PU.1 genomic DNA fragment that contains PU.1 gene locus and approximately 35 kb of DNA both 5' and 3' of the coding exons. We also inserted a murine Thy1.1 cDNA reporter gene31 between exons 4 and 5 using a RecA-based homologous recombination method.32 This 91-kb PU.1-Thy1.1 construct confers high-level, cell line-specific expression in stable transfection assays. Furthermore, the same construct directs tissue-specific reporter gene expression in transgenic mice in a manner similar to that observed by the endogenous PU.1 gene. These results indicate that distal elements located more than 2 kb from the transcription start site are required for proper expression of the PU.1 gene in vivo. Similar results were recently obtained for another gene expressed in very early hematopoietic cells, CD34.36 In the case of CD34, a minigene that included 5 kb of the CD34 promoter and a 3' enhancer, both of which were active in transient transfection assays,47 failed to express CD34 in transgenic mice. However, a 160-kb PAC clone expressed CD34 very efficiently in vivo,36 and recent studies indicate that the distal elements required for CD34 expression are likely to be located approximately 18 kb 5' of the transcription start site (Y. O. and D.G.T., unpublished results). Because we initially planned to use FACS analysis to detect Thy1.1 protein expression, an IRES sequence that provides the binding site for translation initiation was placed in front of the Thy1.1 reporter cDNA. Unfortunately, we found that the IRES sequence was spliced out as a result of a cryptic splice donor site in the murine PU.1 exon 4 and a cryptic splice acceptor at the junction of Thy1.1 exon 1 and intron 1 (Figure 2B). As a result, we failed to detect Thy1.1 protein expression in the presence of abundant Thy1.1 mRNA. However, Thy1.1 reporter mRNA was detected in every clone containing the transgene in both U937 and 416B stable cell lines (Figure 2 and data not shown). Different-sized Thy1.1 mRNAs were detected in these stable clones, with a major transcript of 1.4 kb that is about 0.7 kb smaller than the expected size (Figure 2). This 0.7-kb decrease in the apparent size of the transcript matches the size of the IRES sequence plus those sequences from both PU.1 exon 4 and Thy1.1 exon 1. The nature of these transcripts was further verified by the finding of no detectable Thy1.1 mRNA expression in either parental U937 or 416B cell lines or clones without the transgene (Figure 2), while a 1.8-kb Thy1.1 mRNA was observed in the BW5147 T-cell line. In addition to high-level and chromatin-independent reporter gene expression as that observed from the stable cell lines, this 91-kb PU.1 genomic DNA also confers cell type- and tissue-specific gene expression in transgenic mice. Expression of the Thy1.1 reporter gene was detected at high levels in peritoneal macrophages, bone marrow, and spleen, with very low levels in heart and liver (perhaps from contaminating blood or macrophages). No reporter gene expression was observed in kidney or lung. Importantly, the expression of the exogenous transgene was almost identical to that of the endogenous PU.1 gene (Figure 5). These results suggest that all of the elements necessary for proper control of PU.1 expression are contained within this 91-kb PU.1 genomic region. One of the main features for these types of regulatory elements, such
as LCRs, is chromatin opening ability.42,43,45,48-51 When
genes in a chromosome are actively transcribed, they have an open
configuration that allows transcription regulators to bind to and are
therefore sensitive to DNase I treatment. To map specific
transcriptional regulatory regions in this 91-kb PU.1 locus, we
searched through an approximately 50-kb region spanning from 20 kb
upstream to 10 kb downstream of the murine PU.1 gene. We identified 3 clusters of DNase I HSs, located Recent studies have shown that LCRs are both distance and orientation dependent52 and that the DNase I HSs work together to confer chromatin opening as well as tissue-specific cell-type transcriptional activation. Further studies performed in vivo in transgenic mice will be required to determine the specific functions of the PU.1 DNase I HSs. If these DNase I HSs are shown to be the equivalent of myeloid-specific LCR-like elements, they could potentially provide powerful tools for directing heterologous gene expression for gene therapy applications in acute myeloid leukemia.
We thank Zheng Pan for suggestions with DNase I HS studies, Xiangdong Yang and Nathaniel Heintz for sharing information regarding targeted modification of the P1 by homologous recombination in bacteria, laboratory mates from the Tenen and Zhang laboratories for their support, and Mary Singleton and Alison Lugay for expert assistance with preparation of the manuscript.
Submitted February 13, 2001; accepted July 11, 2001.
Supported by grants CA41456 (D.G.T.) and AI30656 (R.A.M.).
Y. L. and Y. O. contributed equally to this work.
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked "advertisement" in accordance with 18 U.S.C. section 1734.
Reprints: Daniel G. Tenen, Harvard Institutes of Medicine, Rm 954, 77 Ave Louis Pasteur, Boston, MA 02115; e-mail: dtenen{at}caregroup.harvard.edu.
1. Klemsz MJ, McKercher SR, Celada A, Van Beveren C, Maki RA. The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene. Cell. 1990;61:113-124[CrossRef][Medline] [Order article via Infotrieve]. 2. Moreau-Gachelin F, Ray D, Mattei MG, Tambourin P, Tavitian A. The putative oncogene Spi-1: murine chromosomal localization and transcriptional activation in murine acute erythroleukemias. Oncogene. 1989;4:1449-1456[Medline] [Order article via Infotrieve].
3.
Tenen DG, Hromas R, Licht JD, Zhang D-E.
Transcription factors, normal myeloid development, and leukemia.
Blood.
1997;90:489-519
4.
Pahl HL, Scheibe RJ, Zhang D-E, et al.
The proto-oncogene PU.1 regulates expression of the myeloid-specific CD11b promoter.
J Biol Chem.
1993;268:5014-5020
5.
Scott EW, Simon MC, Anastai J, Singh H.
The transcription factor PU.1 is required for the development of multiple hematopoietic lineages.
Science.
1994;265:1573-1577 6. McKercher SR, Torbett BE, Anderson KL, et al. Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities. EMBO J. 1996;15:5647-5658[Medline] [Order article via Infotrieve].
7.
Zhang D-E, Hetherington CJ, Chen HM, Tenen DG.
The macrophage transcription factor PU.1 directs tissue specific expression of the macrophage colony stimulating factor receptor.
Mol Cell Biol.
1994;14:373-381
8.
Hohaus S, Petrovick MS, Voso MT, Sun Z, Zhang D-E, Tenen DG.
PU.1 (Spi-1) and C/EBP
9.
Smith LT, Hohaus S, Gonzalez DA, Dziennis SE, Tenen DG.
PU.1 (Spi-1) and C/EBP
10.
Iwama A, Zhang P, Darlington GJ, McKercher SR, Maki RA, Tenen DG.
Use of RDA analysis of knockout mice to identify myeloid genes regulated in vivo by PU.1 and C/EBP
11.
Anderson KL, Smith KA, Conners K, McKercher SR, Maki RA, Torbett BE.
Myeloid development is selectively disrupted in PU.1 null mice.
Blood.
1998;91:3702-3710
12.
Anderson KL, Smith KA, Perkin H, et al.
PU.1 and the granulocyte- and macrophage colony-stimulating factor receptors play distinct roles in late-stage myeloid cell differentiation.
Blood.
1999;94:2310-2318 13. DeKoter RP, Walsh JC, Singh H. PU.1 regulates both cytokine dependent proliferation and differentiation of granulocyte/macrophage progenitors. EMBO J. 1998;17:4456-4468[CrossRef][Medline] [Order article via Infotrieve].
14.
Galson DL, Hensold JO, Bishop TR, et al.
Mouse
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Abstract
Introduction
/
can
induce PU.1 gene expression in as short as 4 hours, suggesting a direct
role for regulation of the PU.1 gene.
), bone marrow (BM), and
spleen and weakly in liver and lung. The hybridization observed in both
nontransgenic and transgenic thymus is derived from the endogenous
murine Thy1 gene, which is expressed strongly in thymus, weakly in
spleen and heart, and undetectable in the other tissues examined and
indicated by an arrow on the right of the panel. Similar results were
obtained with a second transgenic founder line. (B) Northern
blot analysis of fractionated cells demonstrate myeloid- and B
cell-specific expression of the PU.1 transgene. Cells from the P1
transgenic line shown in panel A were fractionated into
B220+ (B cells); Gr-1+ (granulocytes);
Mac-1+ (granulocytes and macrophages); and CD4+
and/or CD8+ (T-cell) populations as described in
"Materials and methods." Murine Thy1.1, PU.1, and GAPDH RNAs were
detected by Northern blot analysis as described for panel A. The
endogenous murine Thy1 mRNA species was detected in the
CD4+/CD8+ lane slightly below the position of
the second fastest migrating exogenous transgene band, as indicated by
the arrow to the right of the panel. The purity of cells for each
lineage was more than 95% for Gr-1+ cells and more than
90% for Mac-1+, B220+, and
CD4+/CD8+ cells. (C) RT-PCR analysis
demonstrates expression of transgenic RNA in Mac-1+ myeloid
cells but not TER119+ erythroid cells. Highly purified
(> 99%) Mac-1+ (lane 1) and TER119+ cells
(lane 2) were isolated by FACS. Complementary DNA from 200 cells was
used in each RT-PCR reaction. Transgene-derived mRNA was detected by a
combination of 5' PU.1 sense and 3' Thy1.1 antisense primers. GATA-1
and GAPDH RT-PCR were performed as controls for RNA integrity.