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Blood, Vol. 98, Issue 13, 3717-3726, December 15, 2001
Data collection for: Longo et al, Vol 98, Issue 13, 3717–3626 Supplemental Videos: The videos depict dynamic distribution of CD9 during TC-EC interactions, also shown in Figure 6 of the article. QuickTime Video 1: Dynamic distribution of melanoma CD9 during TC-EC interactions See also Figure 6A. A375 melanoma cells were transfected with GFP-tagged CD9 and allowed to invade a confluent EC monolayer. The cellular distribution of CD9-GFP was assessed by time-lapse confocal microscopy every 3 minutes. The focal plane corresponding to TC-EC contact is shown (Z = 0
QuickTime Video 2: Dynamic distribution of endothelial CD9 during TC-EC interactions See also Figure 6B. ECs were transfected with GFP-tagged CD9 and allowed to grown onto collagen gels to confluence. A375 melanoma cells were allowed to invade the EC monolayer, and the distribution of endothelial CD9-GFP was observed by confocal microscopy. The video shows a time-lapse experiment of the TC insertion where the fluorescence and DIC images have been merged. Note that no redistribution of CD9 is seen at initial time points where TC is adhered to the luminal surface of the ECs but only at the time of insertion.
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| Copyright © 2009 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
m). At times 0 and 24 minutes, a projection of the fluorescence signal of the different sections, as well as the DIC image, is shown.