SEARCH:   Advanced

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kiani, A. Articles by Rao, A. Search for Related Content PubMed PubMed Citation Articles by Kiani, A. Articles by Rao, A. Related Collections Immunobiology Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 September 2001, Vol. 98, No. 5, pp. 1480-1488 IMMUNOBIOLOGY Regulation of interferon- gene expression by nuclear factor of activated T cells Alexander Kiani, Francisco J. García-Cózar, Ivonne Habermann, Stefanie Laforsch, Toni Aebischer, Gerhard Ehninger, and Anjana Rao From the Department of Pathology, Harvard Medical School, and The Center for Blood Research, Boston, MA; the Department of Medicine I, University Hospital Carl Gustav Carus, Dresden University of Technology; and the Max-Planck-Institute for Infection Biology, Berlin, Germany.     Abstract Top Abstract Introduction Materials and methods Results Discussion References Transcription factors of the nuclear factor of activated T cells (NFAT) family are thought to regulate the expression of a variety of inducible genes such as interleukin-2 (IL-2), IL-4, and tumor necrosis factor-. However, it remains unresolved whether NFAT proteins play a role in regulating transcription of the interferon-  (IFN-) gene. Here it is shown that the transcription factor NFAT1 (NFATc2) is a major regulator of IFN- production in vivo. Compared with T cells expressing NFAT1, T cells lacking NFAT1 display a substantial IL-4-independent defect in expression of IFN- mRNA and protein. Reduced IFN- production by NFAT1/× IL-4/ T cells is observed after primary in vitro stimulation of naive CD4+ T cells, is conserved through at least 2 rounds of T-helper cell differentiation, and occurs by a cell-intrinsic mechanism that does not depend on overexpression of the Th2-specific factors GATA-3 and c-Maf. Concomitantly, NFAT1/× IL-4/ mice show increased susceptibility to infection with the intracellular parasite Leishmania major. Moreover, IFN- production in a murine T-cell clone is sensitive to the selective peptide inhibitor of NFAT, VIVIT. These results suggest that IFN- production by T cells is regulated by NFAT1, most likely at the level of gene transcription. (Blood. 2001;98:1480-1488) © 2001 by The American Society of Hematology.     Introduction Top Abstract Introduction Materials and methods Results Discussion References In response to antigen stimulation, cells of the immune system initiate the expression of activation-induced genes through the coordinate action of transcription factors at gene regulatory elements. The nuclear factor of activated T cells (NFAT), originally identified as a nuclear complex binding to the antigen-response element of the IL-2 gene,1 has been implicated in the regulation of various inducible genes, particularly those encoding cytokines and cell surface receptors.2 Sequence inspection, in vitro binding assays, and overexpression studies have identified potential binding sites for NFAT in the promoters and enhancers of numerous genes, including those encoding interleukin-2 (IL-2), IL-3, IL-4, IL-5, tumor necrosis factor  (TNF-), granulocyte macrophage-colony-stimulating factor (GM-CSF), CD40L, FasL, CD25, b-type natriuretic peptide, and the adipocyte-specific protein aP2.2-5 In general, NFAT is thought to act as a positive regulator of gene expression. For instance, IL-2 production is profoundly compromised in patients unable to generate functional NFAT complexes,6 and peptides based on the calcineurin-docking site of NFAT proteins, which specifically inhibit the activation of NFAT, impair the production of IL-2 and other cytokines in vitro.7 To date, 4 calcium-regulated members of the NFAT family (NFAT1-4; also known as NFATc1-4 and hereafter abbreviated NFAT), with distinct but overlapping tissue distributions, have been identified.8-15 A fifth protein, NFAT5/TonEBP,16,17 is regulated by osmotic shock. NFAT3 is expressed predominantly outside the immune system, whereas NFAT1 (NFATp, NFATc2), NFAT2 (NFATc, NFATc1), and NFAT4 (NFATx, NFATc3) can be found in immune cells including T cells, B cells, natural killer (NK) cells, macrophages, and mast cells.2,10,18-21 Although NFAT proteins are expressed in multiple cell types, their regulation has been extensively studied in T and B cells, where they are present as phosphoproteins in the cytoplasm of resting cells. After cell stimulation, 4 consecutive steps lead to the activation of NFAT: dephosphorylation by the calcium-dependent phosphatase calcineurin, a process inhibited by the immunosuppressive drugs cyclosporin A and FK50622-26; translocation into the nucleus18,23,27; binding to specific DNA elements in the regulatory regions of target genes, which occurs in physical or functional cooperation with other transcription factors including AP-1 (Fos/Jun), c-Maf (Maf), and GATA-family proteins2,4,22,28-30; and interaction with known or putative coactivator proteins such as p300, CBP, and NIP-45.31-33 After antigen stimulation, CD4+ T cells differentiate into at least 2 types of effector cells that differ as to the pattern of cytokines they produce on restimulation.34-36 Th1 cells are defined by the production of interferon- (IFN-) and mediate predominantly cellular immune responses, whereas the signature cytokines of Th2 cellsIL-4, IL-5, and IL-13are involved in allergic reactions and immune responses against helminthic parasites. In a naive precursor cell, Th1 or Th2 cell differentiation is initiated when prolonged stimulation through the T-cell receptor occurs together with activation of the IL-12/STAT4 or IL-4/STAT6 signaling pathways, respectively.34,36-40 The downstream events in the differentiation process are not completely elucidated, but they involve cell-specific chromatin remodeling.41 The pathophysiologic significance of Th1/Th2 imbalance has been documented in recent years for a number of diseases in humans and mice, and Th1/Th2 balance can be affected by the expression levels of various intracellular proteins including NFAT1, NFAT2, and NFAT4; JNK1 and JNK2; BCL-6; Maf; GATA-3; and the recently identified T box transcription factor T-bet.42-52 We and others42-44,53 have previously shown that T cells isolated from NFAT1-deficient mice have a strong bias to differentiate into Th2 cells. Detailed analysis traced this phenotype to the fact that NFAT1/ T cells produce increased levels of IL-4 relative to NFAT1+/+ cells as a result of prolonged expression of IL-4 mRNA.44 Naive NFAT1/ T cells, differentiated and restimulated in vitro under unskewed conditions, also transcribed significantly less IFN- mRNA than naive NFAT1+/+ T cells44; however, in this case the secondary effects of IL-4 overproduction made it difficult to determine whether the loss of NFAT1 directly affected the expression of IFN- and other cytokines. In particular, IL-4 and the Th2-specific factors Maf and GATA-3, besides promoting Th2 differentiation, are known to down-regulate the expression of IFN- and possibly other Th1 cytokines35,36,50,51; thus, increased IL-4 production by NFAT1/ cells would have a confounding effect. Therefore, a complete analysis of cytokine expression in NFAT1-deficient mice required additional investigation. To eliminate the skewing effects caused by IL-4 overexpression in NFAT1/ mice, we bred these mice to IL-4/ mice54 and compared cytokine expression by NFAT1/ IL-4/ versus NFAT1+/+ IL-4/ T cells. Here we show that, in the absence of endogenous overproduction of IL-4, Th2 differentiation in NFAT1/ cells is essentially normal. In contrast, CD4+ T cells lacking NFAT1 display a pronounced, cell-intrinsic, IL-4-independent defect in IFN- production that most likely reflects a requirement for NFAT1 in the acute phase of transcription of the IFN- gene. This is confirmed by our demonstration that IFN- production in the murine T-cell clone CL.7W2 is sensitive to VIVIT, a selective peptide inhibitor of NFAT.7 Our results provide strong evidence for an in vivo role of NFAT1 in IFN- gene transcription.     Materials and methods Top Abstract Introduction Materials and methods Results Discussion References Mice NFAT1/ and NFAT1+/+ mice,42 on a mixed C57BL/6 and 129Sv/J background, were bred to IL-4/ mice54 (Jackson Laboratories, Bar Harbor, ME) to generate NFAT1/ IL4/ double-knockout and NFAT1+/+ IL-4/ control mice. All mice were maintained in pathogen-free conditions in barrier facilities at the Center for Animal Resources and Comparative Medicine, Harvard Medical School, and were used at age 8 to 12 weeks. Cell culture conditions and reagents Cell culture was performed in Dulbecco modified Eagle medium supplemented with 10% fetal calf serum (FCS), L-glutamine, streptomycin-penicillin, nonessential amino acids, sodium pyruvate, vitamins, HEPES (all from GIBCO, Gaithersburg, MD), and 5 × 105 M 2-mercaptoethanol (Sigma, St Louis, MO). Single-cell suspensions were prepared from lymph node (inguinal, axillary, brachial, cervical) and spleen cells. CD4+ T cells were purified using magnetic beads (Dynabeads L3T4; Dynal, Lake Success, NY). In selected experiments, CD4+ T cells were further purified by cell sorting in Mel-14hi CD4+ T cells, as described.44 All experiments were performed in parallel using equal numbers of cells from NFAT1+/+ IL-4/ and NFAT1/ IL-4/ mice. For primary stimulation, purified CD4+ T cells (1 × 106/mL) were stimulated in vitro for the indicated time points with 1 µg/mL plate-bound anti-CD3 (2C11; Pharmingen, San Diego, CA) or the combination of 10 ng/mL IL-12 (courtesy of M. O'Donnell, Genetics Institute, Cambridge, MA) and 50 ng/mL IL-18 (gift of M. Su, Vertex Pharmaceuticals, Cambridge, MA). In vitro differentiation assays were performed as described.44 Briefly, purified CD4+ T cells (1 × 106/mL) were stimulated with 1 µg/mL plate-bound anti-CD3 alone (default conditions) or in the presence of 5 ng/mL IL-12 or the indicated amounts of IL-4 (IL-4 was added as supernatant from the cell line I3L6 transfected with a constitutively expressed murine IL-4 cDNA55). All cultures were supplemented with 20 U/mL IL-2 (Collaborative Biomedical Products, Bedford, MA) after 24 hours, and fresh media (30% of the initial volume) was added after 48 hours of stimulation. After 4 days, cells were extensively washed, rested for 24 to 48 hours in IL-2 (20 U/mL), counted, and restimulated at 1 × 106 cells/mL with 1 µg/mL plate-bound anti-CD3 for the indicated time points. For mixing experiments, NFAT1+/+ IL-4/ or NFAT1/ IL-4/ CD4+ T cells were labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE) (Molecular Probes, Eugene, OR) before differentiation, as described.56,57 Briefly, 1 × 107 cells/mL were incubated with 10 µM CFSE for 8 minutes at room temperature. To stop labeling, one volume of cold FCS was added, and cells were washed 3 times with culture media. Cells were then differentiated as described above, either alone or in mixed cultures together with unlabeled cells. Transfections Plasmid DNA (0.75 µg/106 cells) was introduced in Cl.7W2 cells58 by electroporation in serum-free medium in 0.4-cm cuvettes with settings of 250 V and 950 µF, using a Bio-Rad Gene Pulser II (Hercules, CA). The day after transfection, cells were stimulated for intracellular cytokine staining as indicated. Enzyme-linked immunosorbent assay and intracellular cytokine staining Culture supernatants of cells activated as described above were collected at 24 hours (secondary stimulation) or 48 hours (primary stimulation), and cytokine levels for IFN-, IL-2, and IL-5 were analyzed by enzyme-linked immunosorbent assay (ELISA) using standard protocols and monoclonal antibodies specific for murine (m) IFN-, mIL-2, and mIL-5 (courtesy of A. Clausell, Pharmingen). Calculated values are expressed as mean ± SEM. For intracellular cytokine staining, differentiated T cells or Cl.7W2 cells were stimulated with 20 nM phorbol 12-myristate 13-acetate (PMA) and 1 µM ionomycin for 4 to 5 hours, the last 2 hours in the presence of 10 µg/mL Brefeldin A (Sigma). After harvest, cells were washed with cold phosphate-buffered saline (PBS), fixed for 10 minutes in 4% paraformaldehyde, washed twice with PBS, and permeabilized with PBS/FCS containing 0.5% saponin (Sigma). Staining was performed for 30 minutes at room temperature with allophycocyanin- or phycoerythrin-conjugated control immunoglobulin and anti-mIFN- antibody (Pharmingen) diluted in 0.5% saponin buffer. After staining, cells were washed twice with 0.5% saponin buffer and once with PBS and then were analyzed on a flow cytometer (FACScalibur and FACScan; Becton Dickinson Immunocytometry Systems, San Jose, CA). RNase protection assay and Northern blot analysis At the indicated time points after stimulation, cells were harvested and total cellular RNA was immediately extracted (Ultraspec, Houston, TX). Cytokine mRNA levels were analyzed by RNase protection assay using the RiboQuant multiprobe kit (Pharmingen) following the instructions of the manufacturer. Briefly, equal amounts of target RNA (1-5 µg) were hybridized overnight to a [32P]-labeled RNA probe that had been synthesized in vitro from a multicytokine template set, after which free probe and other single-stranded RNA were digested with RNases. Protected fragments were purified and resolved on a 6% denaturing polyacrylamide gel and visualized by autoradiography. Cytokine transcripts were identified by the lengths of the respective fragments. RNA loading was estimated by measuring intensities of protected fragments encoding 2 housekeeping genes, L32 and GAPDH, included in the multitemplate set as internal controls. For Northern blot analysis, total RNA was separated by gel electrophoresis (10 µg/lane) and transferred to a nylon membrane (Nytran; Schleicher & Schuell, Keene, NH). cDNA fragments for Northern hybridization were purified from plasmids containing the full-length murine c-Maf30 and human GATA-359 cDNAs, respectively. Infections with Leishmania major L major LRC-L137 clone V121 promastigotes60 were cultured in semidefined medium 7961 supplemented with 10% FCS (Seromed, Berlin, Germany) to stationary phase at 26°C. The cells were harvested and washed twice with PBS and finally resuspended in PBS at 108 parasites/mL. Thirty microliters of this suspension was injected into the left hind footpad. Footpad thickness was recorded at the indicated times using a caliper. The difference to the uninfected foot was calculated and plotted as the mean ± SD. Parasite burden in draining lymph nodes was estimated by limiting-dilution analysis. Single-cell suspensions of lymph node cells were serially diluted by a factor of 3.18 in 4 replicates in semidefined medium 79. Parasite growth was recorded microscopically after 4 to 6 days of culture. Total number of parasites per lymph node was calculated from the highest dilution at which a minimum of 1 of 4 replicates still contained parasites and the total number of lymph node cells in suspension.     Results Top Abstract Introduction Materials and methods Results Discussion References All the experiments described in this article were performed using IL-4-deficient T cells that either expressed or lacked the transcription factor NFAT1. For simplicity, therefore, the cells are occasionally referred to as NFAT1/ or NFAT1+/+, with the common IL-4/ background assumed. NFAT1 regulates IFN- expression through an IL-4-independent mechanism in T cells To assess the effect of NFAT1 on cytokine production in the absence of the confounding effects of IL-4, we purified CD4+ T cells from NFAT1+/+ IL-4/ and NFAT1/ IL-4/ spleen and lymph node cells and differentiated them in vitro with plate-bound anti-CD3, in the absence of added cytokines or antibodies (default conditions). Four days after stimulation, cells were washed, rested, and restimulated with plate-bound anti-CD3, after which levels of cytokine transcripts were quantified by RNase protection assay (Figure 1A). Under these conditions, both NFAT1+/+ IL-4/ and NFAT1/ IL-4/ T cells expressed barely detectable levels of mRNAs encoding the Th2 cytokines IL-5 and IL-13 and, as expected, no mRNA encoding IL-4 (Figure 1A, left panel). This result confirmed our earlier conclusion that Th2 differentiation in NFAT1/ cells was completely dependent on IL-4 because it was inhibited by inclusion of a neutralizing anti-IL-4 antibody.44,53 In the same experiment, NFAT1/ IL-4/ T cells expressed considerably lower levels of mRNA encoding GM-CSF and the Th1 cytokine IFN- than NFAT1+/+ IL-4/ T cells (Figure 1A). In contrast, there was no consistent difference in the expression of IL-2, FasL, and TNF- between NFAT1/ IL-4/ and NFAT1+/+ IL-4/ T cells (Figure 1A-B, right panel). Analysis of supernatants of restimulated cells by ELISA confirmed that IFN- production by NFAT1/ IL-4/ T cells was profoundly compromised (Figure 1B, left panel). These experiments suggested that NFAT1 acted independently of IL-4 to regulate IFN- and possibly GM-CSF expression during T-cell differentiation. View larger version (33K): [in this window] [in a new window]   Figure 1. Reduced IFN- production by NFAT1/ IL-4/ T-helper cells. (A) NFAT1/ IL-4/ T cells show diminished levels of IFN- mRNA. CD4+ T cells from NFAT1+/+ IL-4/ and NFAT1/ IL-4/mice were cultured for 4 days with anti-CD3 and IL-2, rested for 48 hours, and restimulated with anti-CD3. Differentiation and restimulation were performed under neutral conditions, without the addition of antibodies and cytokines other than IL-2. Six hours after restimulation, total cellular RNA was isolated and analyzed by RNase protection assay for transcript levels of the indicated cytokines. (B) NFAT1/ IL-4/ T cells show diminished production of IFN- protein. NFAT1+/+ IL-4/ and NFAT1/ IL-4/ CD4+ T cells were cultured as in panel A. Supernatants were collected 24 hours after restimulation, and levels of IFN- and IL-2 were determined by ELISA. Values are expressed as the means ± SEM of 9 (IFN-) or 3 (IL-2) independent experiments. As shown previously, Th2 skewing of NFAT1/ T cells is preceded by prolonged expression of the IL-4 gene in response to the differentiation-inducing stimulation. However, because of the endogenous overproduction of IL-4, other potential mechanisms affecting T-helper cell differentiation in NFAT1/ cells could not be tested in these experiments. We examined the effect of IL-4 on the differentiation of IL-4/ T cells that expressed or lacked NFAT1. CD4+ T cells were purified from NFAT1+/+ IL-4/ and NFAT1/ IL-4/ mice and were stimulated as described above, in the absence or presence of IL-4 (0 to 1000 U/mL). Inclusion of IL-4 induced comparable Th2 differentiation in both the NFAT1+/+ IL-4/ and the NFAT1/ IL-4/ T cells, characterized by increased IL-5, IL-10, and IL-13 mRNA and down-regulation of IFN- mRNA (Figure 2A). A concomitant increase in IL-5 secretion and down-regulation of IFN- secretion was also observed (Figure 2B). Notably, considerably lower levels of IL-4 sufficed for almost total extinction of IFN- production in NFAT1/ IL-4/ relative to NFAT1+/+ IL-4/ cells (compare lanes 2 and 6 in Figure 2A and black and hatched bars in Figure 2B, lower panel). View larger version (50K): [in this window] [in a new window]   Figure 2. IL-4 promotes Th2 differentiation of NFAT1/ IL-4/ T cells. (A) IL-4 treatment up-regulates mRNAs encoding Th2 cytokines and down-regulates IFN- mRNA in both NFAT1/ IL-4/ () and NFAT1+/+ IL-4/ () cells. CD4+ T cells from NFAT1+/+ IL-4/ and NFAT1/ IL-4/ mice were cultured for 4 days with anti-CD3 and IL-2 in the presence of IL-4 (0-1000 U/mL), rested for 48 hours, and restimulated with anti-CD3. Six hours after restimulation, total cellular RNA was isolated and analyzed by RNase protection assay for transcript levels of the indicated cytokines. (B) IL-4 treatment up-regulates IL-5 production and down-regulates IFN- production in both NFAT1/ IL-4/ and NFAT1+/+ IL-4/ cells. NFAT1+/+ IL-4/ and NFAT1/ IL-4/ CD4+ T cells were cultured as in panel A. Supernatants were collected 24 hours after restimulation, and levels of IFN- and IL-5 were determined by ELISA. Values are expressed as the means ± SEM of 3 independent experiments. NFAT1 regulates IFN- expression by a cell-intrinsic mechanism independent of the subset-specific factors GATA-3 and Maf As a transcription factor, NFAT1 is known to induce the expression of numerous cytokines and cell surface molecules,2 some of which could potentially affect the development of IFN--producing cells during T-cell differentiation. To examine the involvement of soluble or membrane-bound factors that might influence IFN- production, we performed mixing experiments in which NFAT1/ IL-4/ and NFAT1+/+ IL-4/ T cells were differentiated either alone or together. To distinguish the 2 types of T cells in the mixed populations, we labeled one of the populations with the fluorescent dye CFSE.57 After differentiation, IFN- production by NFAT1/ IL-4/ and NFAT1+/+ IL-4/ T cells was independently quantified, either by restimulation followed by intracellular cytokine staining (Figure 3A) or by separating differentiated cells by cell sorting, restimulating the separated populations, and measuring IFN- by ELISA assays of cell supernatants (Figure 3B). As shown in Figure 3, NFAT1/ IL-4/ T cells consistently produced less IFN- than NFAT1+/+ IL-4/ T cells, regardless of whether they were labeled with CFSE and whether they were cultured alone or together with the other cell type. This result suggested that NFAT1 promotes IFN- production by differentiated T cells through a cell-intrinsic mechanism rather than indirectly by inducing soluble or membrane-bound factors. View larger version (29K): [in this window] [in a new window]   Figure 3. NFAT1 promotes IFN- production through a cell-intrinsic mechanism. Both panels show that NFAT1/ IL-4/ T cells produce less IFN-, regardless of whether or not they are mixed with NFAT1+/+ IL-4/ T cells. (A) Intracellular staining for IFN--producing cells. CD4+ T cells from NFAT1+/+ IL-4/ and NFAT1/ IL-4/ mice were left unlabeled or were labeled with the fluorescent tracker dye CFSE, then differentiated by culturing them separately or as a 1:1 mixture with anti-CD3 and IL-2 for 4 days. After restimulation for 4 hours with PMA and ionomycin, IFN- production was analyzed at a single-cell level by intracellular cytokine staining, without further separation of the mixed-cell populations. Numbers on the right refer to the percentage of cells in each quadrant, relative to the total number of NFAT1+/+ IL-4/ (italics) or NFAT1/ IL-4/ (nonitalics) cells. Note that cell division during the differentiation period can be followed over 6 to 7 generations by the decrease of CFSE fluorescence intensity. Note also that CFSE labeling does not cause significant changes in the proportion of cells expressing IFN-. (B) Levels of secreted IFN-. Cells were treated as in panel A, except that mixed populations were separated by FACS sorting immediately before they were restimulated. Twenty-four hours after restimulation, IFN- secreted into the supernatants was analyzed by ELISA. (left) Cells were cultured separately. Again, note that CFSE labeling does not cause significant changes in levels of IFN- production. (right) Cells were cultured as mixed populations and separated by sorting; the labeled populations are indicated. Note that cells in the mixed cultures, which underwent the sorting procedure, showed a considerable decrease in their levels of IFN- production regardless of whether they expressed or lacked NFAT1 (compare the scale of the y-axis in the left and right panels). Nevertheless, the relative decrease in IFN- expression by NFAT1/ IL-4/ versus NFAT1+/+ IL-4/ T cells was maintained even after cell sorting (right panel). We asked whether reduced IFN- production in differentiated NFAT1/ IL-4/ T cells was secondary to increased expression of Maf or GATA-3. In addition to promoting Th2 differentiation, overexpression of these transcription factors in T cells results in IL-4-independent inhibition of IFN- production.50,51 NFAT1/ IL-4/ and NFAT1+/+ IL-4/ T cells were differentiated in the absence or presence of IL-4 (1000 U/mL), and levels of Maf and GATA-3 were assessed by Northern and Western blotting. As expected, Maf and GATA-3 transcripts (Figure 4) and GATA-3 protein (data not shown) were undetectable in T cells cultured without IL-4 (Figure 4, lanes 1 and 2) but were present in T cells differentiated along the Th2 pathway by exposure to IL-4 (Figure 4, lanes 3 and 4). However, there was no difference in Maf and GATA-3 expression in NFAT1/ IL-4/ versus NFAT1+/+ IL-4/ T cells (Figure 4, compare lanes 1 and 3 to lanes 2 and 4, respectively). Thus, the decrease in IFN- production by differentiated NFAT1/ IL-4/ T cells is not mediated through increases in the expression levels of Maf or GATA-3. View larger version (60K): [in this window] [in a new window]   Figure 4. Decreased production of IFN- by NFAT1/ IL-4/ T cells cannot be explained by overexpression of Maf or GATA-3. CD4+ T cells from NFAT1+/+ IL-4/ and NFAT1/ IL-4/ mice were cultured for 4 days with anti-CD3 and IL-2, either under neutral conditions (lanes 1 and 2) or in the presence of 1000 U/mL IL-4 to induce Th2 differentiation (lanes 3 and 4). After harvesting the cells, total RNA was extracted, and Northern blot analysis was performed as described in "Materials and methods." Ethidium bromide staining of the 28S bands confirmed equal loading of RNAs isolated from NFAT1/ IL-4/ and NFAT1+/+ IL-4/ cells (data not shown). Results of 1 of 2 identical experiments are shown. NFAT1 is required for optimal IFN- production by naive T-helper cells and throughout T-cell differentiation The presence of IL-12 strongly induces Th1 differentiation and is necessary for optimal IFN- production by effector T cells.62 The experiments reported above involved purified CD4+ T cells and thus did not reflect IL-12-dependent mechanisms. We therefore asked whether the inclusion of IL-12 during differentiation would rescue high levels of IFN- production in NFAT1/ IL-4/ relative to NFAT1+/+ IL-4/ T cells. In the same experiment, we asked whether the IFN- defect of these cells was limited to a restricted time window or was maintained through several rounds of differentiation involving repeated restimulations. To avoid the potentially confounding effects of CD4+ memory or NK1.1 T cells, we used naive T-cell populations (CD4+ Mel-14hi) of NFAT1/ IL-4/ and NFAT1+/+ IL-4/ cells. Cells were cultured in the absence or presence of IL-12 for 2 consecutive rounds of differentiation involving a total of 3 stimulationsthe primary stimulation (depicted as number 1 in Figure 5), the secondary stimulation (number 2) occurring after the first round of differentiation, and the tertiary stimulation (number 3) occurring after the second round of differentiation. After each stimulation, supernatants (Figure 5) and total RNA (data not shown) were analyzed for IFN- expression. View larger version (18K): [in this window] [in a new window]   Figure 5. NFAT1 is required for optimal IFN- production by T cells at all stages of T-cell differentiation. NFAT1 is required for optimal IFN- production by naive and differentiated T-helper cells. NFAT1+/+ IL-4/ and NFAT1/ IL-4/ CD4+ T cells were sorted by FACS for high expression of Mel-14 and were cultured with plate-bound anti-CD3 and IL-2 for 2 consecutive rounds of differentiation, in the absence (top panels) or presence (bottom panels) of IL-12. Before differentiation (stimulation 1, left panel) and after the first (stimulation 2, middle panels) and second (stimulation 3, right panels) rounds of differentiation, cells were stimulated for 48 hours (stimulation 1) or 24 hours (stimulations 2 and 3) with plate-bound anti-CD3, after which IFN- was analyzed in the supernatants of the cells. Note that y-axis scales representing IFN- production are different in all panels. Strikingly, a marked reduction in IFN- production by NFAT1/ IL-4/ relative to NFAT1+/+ IL-4/ cells was already apparent after the first in vitro stimulation of naive T cells (Figure 5, left panel). The NFAT1/ IL-4/ T cells remained capable of differentiation because culture in the absence of IL-12 led to successively increased IFN- production in both NFAT1+/+ IL-4/ and NFAT1/ IL-4/ cells (Figure 5, top panels: note changes in y-axis scales). In both populations, inclusion of IL-12 in the differentiation cultures greatly potentiated IFN- production by the differentiated cells (Figure 5, bottom panels), indicating that NFAT1/ IL-4/ T cells remained responsive to IL-12. However, NFAT1/ IL-4/ T cells consistently produced considerably lower levels of IFN- than NFAT1+/+ IL-4/ cells, despite repeated restimulation in either the absence or presence of IL-12. Thus, NFAT1 is required for optimal production of IFN- not only in a restricted time window, but throughout the differentiation of CD4+ T cells. Decreased resistance of NFAT1/ IL-4/ mice to infection with L major Efficient production of IFN- is critical for resistance to infections with intracellular pathogens, such as L major. To test whether the reduced IFN- production by NFAT1/ IL-4/ T cells had biologically relevant consequences in vivo, we infected NFAT1/ IL-4/ and NFAT1+/+ IL-4/ mice with L major in one hind footpad. As expected from both their IL-4 null and their C57BL/6 and 129/SvJ genetic backgrounds,63 both mouse strains were considerably resistant to the infection and eventually controlled the disease (Figure 6A). However, compared to footpads of NFAT1+/+ IL-4/ mice, footpads of NFAT1/ IL-4/ mice showed a slight but significant increase in size during the course of the infection (Figure 6A). This was paralleled by an approximately 10-fold increase in the number of parasites (Figure 6B; note logarithmic scale), and a 2-fold increase in the number of lymphocytes (data not shown), in the draining lymph nodes of NFAT1/ animals 11 weeks after infection. These results suggested that resistance to infection with L major was reduced in NFAT1/ IL-4/ mice compared to IL-4/ mice and is consistent with reduced IFN- production by NFAT1/ IL-4/ relative to IL-4/ CD4+ T cells. View larger version (17K): [in this window] [in a new window]   Figure 6. Decreased resistance of NFAT1/ IL-4/ mice to infection with L major. (A) NFAT1/ IL-4/ and NFAT1+/+ IL-4/ female mice (10 mice per group) were infected with promastigotes of L major in the left hind footpad. Footpad thickness was recorded at the indicated times using a metric caliper, and the lesion size was calculated as the difference between the injected and the uninjected footpads. Values are shown as means ± SD. (B) NFAT1/ IL-4/ and NFAT1+/+ IL-4/ mice (3 mice per group) were infected with promastigotes of L major as in panel A. Eleven weeks after infection, the number of parasites in the draining popliteal lymph nodes was determined as described in "Material and methods." Values are shown as means ± SD. KO, knockout mice; DKO, double-knockout mice. NFAT1 is required for the acute phase of IFN- gene expression The experiments above suggested that NFAT1 is required for the acute phase of IFN- gene expression in naive T cells. To test this hypothesis further, we asked whether freshly isolated, undifferentiated NFAT1/ IL-4/ T cells would show impaired IFN- production in response to stimuli that do not involve activation of NFAT proteins (Figure 7A). The stimuli chosen were IL-12 and IL-18, cytokines that activate STAT and NFB proteins, respectively, but are not known to activate NFAT.35,36,64 As shown in Figure 7A, NFAT1/ IL-4/ T cells stimulated by anti-CD3 for 48 hours produced substantially lower levels of IFN- than NFAT1+/+ IL-4/ T cells. In contrast, IFN- production in response to combined stimulation with IL-12 and IL-18 was not impaired but was slightly enhanced in NFAT1/ IL-4/ relative to NFAT1+/+ IL-4/ T cells. Thus, NFAT1 appears to be required for acute expression of the IFN- gene in T cells through a specific CD3-mediated pathway, whereas an alternative, IL-12- and IL-18-dependent pathway remains unaffected. View larger version (18K): [in this window] [in a new window]   Figure 7. NFAT1 regulates the acute phase of IFN- production in T-helper cells. (A) Reduced IFN- production by freshly isolated NFAT1/ IL-4/ T-helper cells in response to stimulation with anti-CD3 but not IL-12/IL-18. NFAT1+/+ IL-4/ () and NFAT1/ IL-4/ () CD4+ T cells were stimulated either with plate-bound anti-CD3 (left panel) or a combination of IL-12 and IL-18 (right panel) for 48 hours, after which IFN- was analyzed in the supernatants of the cells. Shown is a representative of 3 (left panel) or 5 (right panel) independent experiments. (B) The selective NFAT inhibitor GFP-VIVIT suppresses IFN- production by a murine T-cell clone. Cl.7W2 cells were transfected with plasmids encoding either GFP or GFP-VIVIT. The next day the cells were either left unstimulated or were stimulated for 5 hours with PMA and ionomycin. IFN- production was analyzed at a single-cell level by intracellular cytokine staining. Dot blots from 1 of 3 similar experiments are shown. Thresholds for IFN- production were set using the unstimulated control. Note that each panel shows transfected (GFP-positive) and nontransfected (GFP-negative) cells of the same sample. To further determine the role of NFAT in the acute phase of IFN- gene expression, we tested whether IFN- production in the murine T-cell clone Cl.7W258 could be inhibited by the selective peptide inhibitor of NFAT, VIVIT (Figure 7B, Table 1). The VIVIT peptide specifically suppresses the activation of NFAT in stimulated T cells by binding with high affinity to its binding site in calcineurin.7 Plasmids encoding green fluorescent protein (GFP) and the fusion protein GFP-VIVIT were introduced into Cl.7W2 cells, and a day later the cells were stimulated with PMA and ionomycin. Five hours after stimulation, IFN- production was analyzed by intracellular cytokine staining, which permitted direct comparison of IFN- production by transfected (GFP-positive) and nontransfected (GFP-negative) cells at a single cell level in the same sample. Transfection efficiency for GFP (5.3%) and GFP-VIVIT (5.7%) was comparable (mean of 3 experiments). Expression of GFP alone did not affect IFN- production by stimulated Cl.7W2 cells (Figure 7B, left lower panel, and data not shown). In contrast, cells expressing GFP-VIVIT produced considerably less IFN-, in comparison with both the nontransfected cells of the same sample and the cells transfected with GFP alone (Figure 7B; compare upper right quadrants in right and left lower panels). Because GFP-VIVIT is estimated to have an IC50 for inhibition of approximately 300 to 500 nM7,26 and is effective only when expressed at relatively high concentrations, we considered for quantification only the cells with GFP fluorescence intensities greater than 100 (upper right quadrants of dot blots in Figure 7B). The inhibitory effect of GFP-VIVIT on IFN- production in this cell population was reflected in the decreased number of IFN--producing cells (decrease of 61.2%, 74.7%, and 75% in experiments 1, 2, and 3, respectively) and in the mean fluorescence intensity of these cells (decrease of 55.5%, 69.3%, and 27.3% in experiments 1, 2, and 3 respectively) (Table 1). The product of these 2 numbers is a measure of total IFN- production by the GFP- or GFP-VIVIT-expressing cells (Table 1, last column). The ratios, which are 82.7%, 92.2%, and 81.9% in experiments 1, 2, and 3, respectively, measure the ability of VIVIT to inhibit IFN- production and therefore measure the dependence of IFN- production on NFAT. We conclude that the NFAT inhibitor VIVIT markedly inhibits IFN- production by stimulated T cells, proving that IFN- gene transcription requires the activation of NFAT.                                View this table: [in this window] [in a new window]   Table 1. The NFAT inhibitor GFP-VIVIT suppresses IFN- production by T cells     Discussion Top Abstract Introduction Materials and methods Results Discussion References In this study, we show that T cells lacking NFAT1 display a substantial, cell-intrinsic defect in IFN- gene expression independent of the down-regulatory effects of IL-4, GATA-3, and Maf. Naive CD4+ T cells from NFAT1/ IL-4/ mice show a marked impairment of IFN- production after primary in vitro stimulation with anti-CD3, a defect that continues to be apparent throughout 2 rounds of subsequent differentiation in vitro. The defect is not restricted to CD4+ T cells; it is also observed after primary in vitro stimulation of CD8+ T cells (F.J.G.-C., A.K., and A.R., unpublished results, 1999). IFN- production in freshly isolated NFAT1/ IL-4/ T cells is normal when they are stimulated with IL-12 and IL-18, demonstrating that NFAT1/ IL-4/ T cells are capable of producing IFN- under conditions that do not involve the acute activation of NFAT. Moreover, IFN- production in normal (NFAT-expressing) T cells is susceptible to the selective NFAT inhibitor GFP-VIVIT. We also show that the effect of NFAT1 on IFN- production is likely to be biologically relevant: despite their highly resistant genetic background (IL-4-null, C57BL/6, and 129/SvJ), enhanced susceptibility of NFAT1/ IL-4/ mice to infection with the intracellular parasite L major was clearly detectable (Figure 6). The fact that NFAT1/ IL-4/ mice were eventually able to control the disease is not surprising considering reports showing that mice on a resistant background treated with IFN-- and IL-4-neutralizing antibodies were only temporarily susceptible to the infection.65 It should be emphasized that the previously shown enhanced susceptibility of NFAT1/ (IL-4+/+) mice to L major could be explained by their IL-4 overproduction and Th2 differentiation,44 which in the present study is excluded. Whereas a role of additional, yet unknown, factors is possible, a causal association to the reduced production of IFN- by NFAT1/ IL-4/ T cells appears to be likely. These results provide the first evidence for the involvement of an NFAT transcription factor in IFN- gene expression in vivo and extend other studies that demonstrated binding and transactivation of IFN- promoter constructs by NFAT in vitro and in cell lines.66-69 NFAT1 regulates acute transcription of the IFN- gene Cytokine gene expression may be divided into 2 phases, a differentiative stage characterized by chromatin remodeling and demethylation of cytokine gene loci, in which T cells become "transcriptionally competent" to express certain cytokine genes,41,56 and a phase of acute gene transcription by the differentiated T cells. The competence phase of IFN- gene transcription is complex. During Th1 differentiation, T-cell receptor- and IL-12/STAT4-dependent signaling pathways are highly interconnected, such that the final levels of differentiated, IFN- producing Th1 cells reflect a balance between permissive factors such as STAT4, JNK2, and T-bet38,40,48,52 versus repressive factors such as STAT6, GATA-3, and Maf.50,51,70 In contrast, the acute transcriptional phase is less complex: differentiated Th1 cells produce IFN- in response to activation through at least 2 independent signaling pathways involving the T-cell receptor and the IL-12 and IL-18 receptors, respectively.36,64,71,72 As discussed in the following sections, our results implicate a role for NFAT1 in the acute phase of IFN- gene transcription. As a transcription factor activated by T-cell-receptor stimulation, NFAT1 is likely to contribute to the T-cell-receptor pathway, but not the STAT4-IL-12/NFB-IL-18 pathway, of acute IFN- induction. That is, NFAT1 might directly regulate IFN- gene transcription in the same way that it is thought to participate directly in transcription of the IL-2, IL-4, and TNF- genes.2 This hypothesis is supported by our observation that T cells lacking NFAT1 are not compromised in their ability to differentiate into IFN--secreting cells but that they display markedly reduced IFN- gene transcription at all stages of differentiation in response to acute stimulation with anti-CD3 (Figure 5). Moreover, IFN- production in anti-CD3-stimulated T cells is highly sensitive to CsA (data not shown) and to the selective NFAT inhibitor GFP-VIVIT (Figure 7B, Table 1), again consistent with the hypothesis that NFAT1 acts during the acute stage of IFN- gene transcription. NFAT1 might bind directly to DNA elements in promoter/enhancer regions of the IFN- gene, or it might rapidly up-regulate secondary transcription factor(s) required for IFN- gene transcription. Our results do not rule out that NFAT1 also regulates IFN- gene expression through a second, developmental mechanism. However, we have excluded certain mechanisms by which NFAT1 might promote transcriptional competence (as distinct from acute transcription) of the IFN- gene. First, as our mixing experiments indicate, NFAT1 does not induce the expression of cytokines or other soluble factors necessary for high-level IFN- production by differentiated T cells (Figure 3). Furthermore, our specific differentiation conditions represent an IL-12-free system, ruling out potential effects of NFAT1 on IL-12, IL-12 receptors, or other IL-12-related mechanisms. Second, NFAT1/ IL-4/ T cells do not show perceptible differences, compared to wild-type cells, in chromatin remodeling of the IFN- gene during peripheral differentiation. The mature DNase I hypersensitivity pattern over 11 kb of the IFN- gene developed identically in NFAT1/ IL-4/ and NFAT1+/+ IL-4/ cells (A.K., S. Agarwal, and A.R., unpublished results, 1998). Finally, NFAT1 did not affect the levels of the Th2-specific transcription factors GATA-3 and Maf (Figure 4), nor was there any difference in expression of the Th1-specific factors ERM73 or T-bet52 in differentiated NFAT1/ IL-4/ versus NFAT1+/+ IL-4/ T cells (X. Yu, A.K., and A.R., unpublished results, 2000). Potential NFAT-dependent regulatory elements in the IFN- gene DNA elements through which NFAT1 might regulate IFN- gene transcription have been extensively studied but not yet definitively identified. Analysis of the human IFN- promoter identified NFAT sites at 280 and 160 relative to the transcription start site.66-69 The 280 NFAT site and a previously identified intronic site may bind both NFAT and Rel, whereas the 160 site has the characteristics of a composite NFAT-AP-1 site.68,69 In chromatin immunoprecipitation assays, which measure the ability of a transcription factor to bind a regulatory element in intact cells in vivo, the IFN- promoter is clearly capable of binding NFAT.83 However in transient reporter assays, mutation of either of the 2 NFAT sites only marginally reduced (approximately 2-fold) the overall inducibility of the IFN- promoter, and mutation of both sites had only a small additional effect.66,68,69 Deletion or mutation of the 280 NFAT site considerably diminished, but did not abolish, calcineurin sensitivity of the IFN- promoter.66,68 Together these results indicate that although NFAT binds strongly and selectively to the proximal IFN- promoter region in vivo, the activity of the isolated promoter is only weakly dependent on NFAT in transient reporter assays. The most proximal region of the IFN- promoter (nucleotides 108 to 40) is thought to play a major role in regulating IFN- promoter activity.74-76 This region contains 2 strong regulatory elements that bind a variety of transcription factors, including AP-1, ATF/CREB, and GATA proteins. In unstimulated cells, the proximal regulatory element (40 to 70) binds CREB and ATF-1, whereas the distal element (78 to 100) binds GATA proteins and AP-1; a weak NFAT site identified at 100 is of questionable functional importance because it is not conserved in the mouse gene.69,74,75 In stimulated cells, the composition of factors binding to the proximal element shifts from CREB and ATF-1 to Jun and ATF-2,75 suggesting that the CsA sensitivity of the 108 to 40 region69,74,75 reflects the known CsA sensitivity of p38 and JNK kinases, which activate Jun and ATF2.77,78 Indeed, p38 MAP kinase has been implicated in the signaling pathway leading to acute transcription of the IFN- gene.79 In transgenic mouse models, neither the proximal nor the distal elements fully recapitulates the behavior of the endogenous IFN- gene; the proximal element shows no activity in CD8 cells and only weak activity in CD4 cells, whereas the activity of the distal element is not up-regulated by differentiation in the presence of IL-12.76 Similarly, a larger fragment of the IFN- promoter shows high constitutive activity in Th2 cells (K. Murphy, personal communication). Together these results suggest strongly that IFN- gene transcription is regulated by distal elements in addition to the proximal promoter. A precedent is provided by the GM-CSF and IL-3 genes, which have weak NFAT-dependent promoters but strong distal enhancers.80,81 The strong NFAT dependence and CsA sensitivity of IFN- gene transcription could be explained either by a major involvement of the 280 NFAT site in the context of endogenous gene expression or by the presence of a distal NFAT-responsive element, which resembles the IL-3 and GM-CSF enhancers in containing multiple binding sites for NFAT. Presumably, other distal regulatory elements control the acute responsiveness of the IFN- gene to IL-12 and IL-18. NFAT1 may act coordinately to promote Th1 differentiation and suppress Th2 differentiation The role of NFAT1 in T-helper cell differentiation has been analyzed in several in vivo models using NFAT1 knockout mice. Targeted disruption of the NFAT1 gene in mice results in a Th2 bias,43,44,53 though this is fairly mild and poorly apparent in 1 of 3 mouse strains tested.82 The mechanism of this Th2 bias remains to be understood, but it is IL-4 dependent (Kiani et al,44 Viola et al,53 and this study), and we have postulated that it might reflect the absence of a NFAT1-regulated negative feedback loop that normally terminates the antigen-induced expression of IL-4.44 The impressive allergic phenotype of mice lacking both NFAT1 and NFAT4 suggests that these 2 NFAT proteins act in concert to down-regulate Th2 responses in vivo.45 In the present study, we have identified a second role for NFAT1activation of IFN- and Th1 responses. It is interesting that both IL-4 suppression and IFN- activation shift the Th1/Th2 balance in favor of Th1 responses, confirming a role for NFAT1 in biasing the immune response toward the Th1 phenotype in normal mice. The relative contributions of IL-4 down-regulation and IFN- up-regulation to NFAT1-mediated control of T-helper cell differentiation still must be determined. Potentially, however, NFAT1 could mediate both effects through a single underlying mechanism, for example by inducing a secondary transcription factor that concurrently up-regulates IFN- expression and down-regulates IL-4 expression.     Acknowledgments We thank A. Abbas, F. Alt, L. Glimcher, K. Murphy, and S. Orkin for plasmids and other reagents; S. Agarwal and J. Aramburu for helpful suggestions; F. Macian for help with densitometry; R. Grewal for technical assistance; and T. Bernickel for animal husbandry.     Footnotes Submitted June 28, 2000; accepted April 20, 2001. Supported by National Institutes of Health grants R01 CA-42471 and P01 AI-35297 (A.R.) and by Deutsche Forschungsgemeinschaft grants KI 605/1-1 (A.K.), KI 605/2-1 (A.K., G.E.), and AE 16/1-3 (T.A.). The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked "advertisement" in accordance with 18 U.S.C. section 1734. Reprints: Anjana Rao, Department of Pathology, Harvard Medical School, The Center for Blood Research, 200 Longwood Ave, Boston, MA 02115; e-mail: arao{at}cbr.med.harvard.edu.     References Top Abstract Introduction Materials and methods Results Discussion References 1. Shaw J-P, Utz PJ, Durand DB, et al. Identification of a putative regulator of early T cell activation genes. Science. 1988;241:202-205[Abstract/Free Full Text]. 2. Rao A, Luo C, Hogan PG. Transcription factors of the NFAT family: regulation and function. Annu Rev Immunol. 1997;15:707-747[CrossRef][Medline] [Order article via Infotrieve]. 3. Schuh K, Twardzik T, Kneitz B, et al. The interleukin 2 receptor  chain/CD25 promoter is a target for nuclear factor of activated T cells. J Exp Med. 1998;188:1369-1373[Abstract/Free Full Text]. 4. Molkentin JD, Lu J-R, Antos CL, et al. A calcineurin-dependent transcriptional pathway for cardiac hypertrophy. Cell. 1998;93:215-228[CrossRef][Medline] [Order article via Infotrieve]. 5. Ho I-C, Kim JH-J, Rooney JW, et al. A potential role for the nuclear factor of activated T cells family of transcriptional regulatory proteins in adipogenesis. Proc Natl Acad Sci U S A. 1998;95:15537-15541[Abstract/Free Full Text]. 6. Feske S, Müller JM, Graf D, et al. Severe combined immunodeficiency due to defective binding of the nuclear factor of activated T cells in T lymphocytes of two male siblings. Eur J Immunol. 1996;26:2119-2126[Medline] [Order article via Infotrieve]. 7. Aramburu J, Yaffe MB, López-Rodriguez C, et al. Affinity-driven peptide selection of an NFAT inhibitor more selective than cyclosporin A. Science. 1999;285:2129-2133[Abstract/Free Full Text]. 8. McCaffrey PG, Luo C, Kerppola TK, et al. Isolation of the cyclosporin-sensitive T cell factor NFATp. Science. 1993;262:750-754[Abstract/Free Full Text]. 9. Northrop JP, Ho SN, Chen L, et al. NF-AT components define a family of transcription factors targeted in T-cell activation. Nature. 1994;369:497-501[CrossRef][Medline] [Order article via Infotrieve]. 10. Hoey T, Sun Y-L, Williamson K, et al. Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins. Immunity. 1995;2:461-472[CrossRef][Medline] [Order article via Infotrieve]. 11. Ho SN, Thomas DJ, Timmerman LA, et al. NFATc3, a lymphoid-specific NFATc family member that is calcium-regulated and exhibits distinct DNA-binding specificity. J Biol Chem. 1995;270:19898-19907[Abstract/Free Full Text]. 12. Masuda ES, Naito Y, Tokumitsu H, et al. NFATx, a novel member of the nuclear factor of activated T cells family that is expressed predominantly in the thymus. Mol Cell Biol. 1995;15:2697-2706[Abstract]. 13. Park J, Takeuchi A, Sharma S. Characterization of a new isoform of the NFAT (nuclear factor of activated T cells) gene family member NFATc. J Biol Chem. 1996;271:20914-20921[Abstract/Free Full Text]. 14. Luo C, Burgeon E, Carew JA, et al. Recombinant NFAT1 (NFATp) is regulated by calcineurin in T cells and mediates transcription of several cytokine genes. Mol Cell Biol. 1996;16:3955-3966[Abstract]. 15. Pan S, Koyano-Nakagawa N, Tsuruta L, et al. Molecular cloning and functional charcterization of murine cDNA encoding transcription factor NFATc. Biochem Biophys Res Commun. 1997;240:314-323[CrossRef][Medline] [Order article via Infotrieve]. 16. López-Rodriguez C, Aramburu J, Rakeman AS, et al. NFAT5, a constitutively nuclear NFAT protein that does not cooperate with Fos and Jun. Proc Natl Acad Sci U S A. 1999;96:7214-7219[Abstract/Free Full Text]. 17. Miyakawa H, Woo SK, Dahl SC, et al. Tonicity-responsive enhancer binding protein, a rel-like protein that stimulates transcription in response to hypertonicity. Proc Natl Acad Sci U S A. 1999;96:2538-2542[Abstract/Free Full Text]. 18. Shaw KT, Ho AM, Raghavan A, et al. Immunosuppressive drugs prevent a rapid dephosphorylation of transcription factor NFAT1 in stimulated immune cells. Proc Natl Acad Sci U S A. 1995;92:11205-11209[Abstract/Free Full Text]. 19. Aramburu J, Azzoni L, Rao A, et al. Activation and expression of the nuclear factors of activated T cells, NFATp and NFATc, in human natural killer cells: regulation upon CD16 ligand binding. J Exp Med. 1995;182:801-810[Abstract/Free Full Text]. 20. Lyakh L, Ghosh P, Rice NR. Expression of NFAT-family proteins in normal human T cells. Mol Cell Biol. 1996;17:2475-2484[Abstract]. 21. Timmerman LA, Healy JI, Ho SN, et al. Redundant expression but selective utilization of nuclear factor of activated T cells family members. J Immunol. 1997;159:2735-2740[Abstract]. 22. Jain J, McCaffrey PG, Miner Z, et al. The T-cell transcription factor NFATp is a substrate for calcineurin and interacts with fos and jun. Nature. 1993;365:352-355[CrossRef][Medline] [Order article via Infotrieve]. 23. Shibasaki F, Price ER, Milan D, et al. Role of kinases and the phosphatase calcineurin in the nuclear shuttling of transcription factor NF-AT4. Nature. 1996;382:370-373[CrossRef][Medline] [Order article via Infotrieve]. 24. Luo C, Shaw KT, Raghavan A, et al. Interaction of calcineurin with a domain of the transcription factor NFAT1 that controls nuclear import. Proc Natl Acad Sci U S A. 1996;93:8907-8912[Abstract/Free Full Text]. 25. Masuda ES, Liu J, Imamura R, et al. Control of NFATx1 nuclear translocation by a calcineurin-regulated inhibitory domain. Mol Cell Biol. 1997;17:2066-2075[Abstract]. 26. Aramburu J, Garcia-Cozar F, Raghavan A, et al. Selective inhibition of NFAT activation by a peptide spanning the calcineurin targeting site of NFAT. Mol Cell. 1998;1:627-637[CrossRef][Medline] [Order article via Infotrieve]. 27. Timmerman LA, Clipstone NA, Ho SN, et al. Rapid shuttling of NF-AT in discrimination of Ca2+ signals and immunosuppression. Nature. 1996;383:837-840[CrossRef][Medline] [Order article via Infotrieve]. 28. Crabtree GR. Generic signals and specific outcomes: signaling through Ca2+, calcineurin, and NF-AT. Cell. 1999;96:611-614[CrossRef][Medline] [Order article via Infotrieve]. 29. Chen L, Glover JN, Hogan PG, et al. Structure of the DNA-binding domains from NFAT, Fos and Jun bound specifically to DNA. Nature. 1998;392:42-48[CrossRef][Medline] [Order article via Infotrieve]. 30. Ho I-C, Hodge MR, Rooney JW, et al. The proto-oncogene c-maf is responsible for tissue-specific expression of interleukin-4. Cell. 1996;85:973-983[CrossRef][Medline] [Order article via Infotrieve]. 31. Garcia-Rodriguez C, Rao A. Nuclear factor of activated T cells (NFAT)-dependent transactivation regulated by the coactivators p300/CREB-binding protein (CBP). J Exp Med. 1998;187:2031-2036[Abstract/Free Full Text]. 32. Avots A, Buttmann M, Chuvpilo S, et al. CBP/p300 integrates Raf/Rac-signaling pathways in the transcriptional induction of NF-ATc during T cell activation. Immunity. 1999;10:515-524[CrossRef][Medline] [Order article via Infotrieve]. 33. Hodge MR, Chun HJ, Rengarajan J, et al. NF-AT-driven interleukin-4 transcription potentiated by NIP-45. Science. 1996;274:1903-1905[Abstract/Free Full Text]. 34. Abbas AK, Murphy KM, Sher A. Functional diversity of helper T lymphocytes. Nature. 1996;383:787-793[CrossRef][Medline] [Order article via Infotrieve]. 35. Murphy KM. T lymphocyte differentiation in the periphery. Curr Opin Immunol. 1998;10:226-232[CrossRef][Medline] [Order article via Infotrieve]. 36. O'Garra A. Cytokines induce the development of functionally heterogenous T helper cell subsets. Immunity. 1998;8:275-283[CrossRef][Medline] [Order article via Infotrieve]. 37. Shimoda K, van Deursen J, Sangster MY, et al. Lack of IL-4-induced Th2 response and IgE class switching in mice with disrupted Stat6 gene. Nature. 1996;380:630-633[CrossRef][Medline] [Order article via Infotrieve]. 38. Thierfelder WE, van Deursen JM, Yamamoto K, et al. Requirement for Stat4 in interleukin-12-mediated responses of natural killer and T cells. Nature. 1996;382:171-174[CrossRef][Medline] [Order article via Infotrieve]. 39. Kaplan MH, Schindler U, Smiley ST, et al. Stat6 is required for mediating responses to IL-4 and for the development of Th2 cells. Immunity. 1996;4:313-319[CrossRef][Medline] [Order article via Infotrieve]. 40. Kaplan MH, Sun Y-L, Hoey T, et al. Impaired IL-12 responses and enhanced development of Th2 cells in Stat-4-deficient mice. Nature. 1996;382:174-177[CrossRef][Medline] [Order article via Infotrieve]. 41. Agarwal S, Rao A. Modulation of chromatin structure regulates cytokine gene expression during T cell differentiation. Immunity. 1998;9:765-775[CrossRef][Medline] [Order article via Infotrieve]. 42. Xanthoudakis S, Viola JPB, Shaw KTY, et al. An enhanced immune response in mice lacking the transcription factor NFAT1. Science. 1996;272:892-895[Abstract]. 43. Hodge MR, Ranger AM, Charles de la Brousse F, et al. Hyperproliferation and dysregulation of IL-4 expression in NF-Atp-deficient mice. Immunity. 1996;4:397-405[CrossRef][Medline] [Order article via Infotrieve]. 44. Kiani A, Viola JPB, Lichtman AH, et al. Down-regulation of IL-4 gene transcription and control of Th2 cell differentiation by a mechanism involving NFAT1. Immunity. 1997;7:849-860[CrossRef][Medline] [Order article via Infotrieve]. 45. Ranger AM, Oukka M, Rengarajan J, et al. Inhibitory function of two NFAT family members in lymphoid homeostasis and Th2 development. Immunity. 1998;9:627-635[CrossRef][Medline] [Order article via Infotrieve]. 46. Yoshida H, Nishina H, Takimoto H, et al. The transcription factor NF-ATc1 regulates lymphocyte proliferation and Th2 cytokine production. Immunity. 1998;8:115-124[CrossRef][Medline] [Order article via Infotrieve]. 47. Dong C, Yang DD, Wysk M, et al. Defective T cell differentiation in the absence of Jnk1. Science. 1998;282:2092-2095[Abstract/Free Full Text]. 48. Yang DD, Conze D, Whitmarsh AJ, et al. Differentiation of CD4+ T cells to Th1 cells requires MAP kinase JNK2. Immunity. 1998;9:575-585[CrossRef][Medline] [Order article via Infotrieve]. 49. Dent AL, Shaffer AL, Yu X, et al. Control of inflammation, cytokine expression and germinal center formation by BCL-6. Science. 1997;276:589-592[Abstract/Free Full Text]. 50. Ho I-C, Lo D, Glimcher LH. c-maf promotes T helper cell type 2 (Th2) and attenuates Th1 differentiation by both interleukin 4-dependent and -independent mechanisms. J Exp Med. 1998;188:1859-1866[Abstract/Free Full Text]. 51. Ouyang W, Ranganath SH, Weindel K, et al. Inhibition of Th1 development mediated by GATA-3 through an IL-4-independent mechanism. Immunity. 1998;9:745-755[CrossRef][Medline] [Order article via Infotrieve]. 52. Szabo SJ, Kim ST, Costa GL, et al. A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell. 2000;100:655-669[CrossRef][Medline] [Order article via Infotrieve]. 53. Viola JPB, Kiani A, Bozza PT, et al. Regulation of allergic inflammation and eosinophil recruitment in mice lacking the transcription factor NFAT1: role of interleukin-4 (IL-4) and IL-5. Blood. 1998;91:2223-2230[Abstract/Free Full Text]. 54. Kühn R, Rajewski K, Müller W. Generation and analysis of interleukin-4 deficient mice. Science. 1991;254:707-710[Abstract/Free Full Text]. 55. Tepper RI, Pattengale PK, Leder P. Murine interleukin-4 displays potent anti-tumor activity in vivo. Cell. 1989;57:503-512[CrossRef][Medline] [Order article via Infotrieve]. 56. Bird JJ, Brown DR, Mullen AC, et al. Helper T cell differentiation is controlled by the cell cycle. Immunity. 1998;9:229-237[CrossRef][Medline] [Order article via Infotrieve]. 57. Lyons AB, Parish CR. Determination of lymphocyte division by flow cytometry. J Immunol Methods. 1994;171:131-137[CrossRef][Medline] [Order article via Infotrieve]. 58. Valge-Archer VE, de Villiers J, Sinskey AJ, et al. Transformation of T lymphocytes by the v-fos oncogene. J Immunol. 1990;145:4355-4364[Abstract]. 59. Ho IC, Vorhees P, Marin N, et al. Human GATA-3: a lineage-restricted transcription factor that regulates the expression of the T cell receptor alpha gene. EMBO J. 1991;10:1187-1192[Medline] [Order article via Infotrieve]. 60. Handman E, Hocking RE, Mitchell GF, et al. Isolation and characterization of infective and non-infective clones of Leishmania tropica. Mol Biochem Parasitol. 1983;7:111-126[CrossRef][Medline] [Order article via Infotrieve]. 61. Brun R, Schönenberger M. Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Acta Trop. 1979;36:289-292[Medline] [Order article via Infotrieve]. 62. Hsieh C-S, Macatonia SE, Tripp CS, et al. Development of TH 1 CD4+ T cells through IL-12 produced by Listeria-induced macrophages. Science. 1993;260:547-549[Abstract/Free Full Text]. 63. Reiner SL, Locksley RM. The regulation of immunity to Leishmania major. Annu Rev Immunol. 1995;13:151-177[CrossRef][Medline] [Order article via Infotrieve]. 64. Robinson D, Shibuya K, Mui A, et al. IGIF does not drive Th1 development but synergizes with IL-12 for interferon- production and activates IRAK and NFB. Immunity. 1997;7:571-581[CrossRef][Medline] [Order article via Infotrieve]. 65. Chatelain R, Mauze S, Varkila K, et al. Leishmania major infection in interleukin-4 and interferon-gamma depleted mice. Parasite Immunol. 1999;21:423-431[CrossRef][Medline] [Order article via Infotrieve]. 66. Brown DA, Nelson FB, Reinherz EL, et al. The human interferon-gamma gene contains an inducible promoter that can be transactivated by tax I and II. Eur J Immunol. 1991;21:1879-1885[Medline] [Order article via Infotrieve]. 67. Campbell PM, Pimm J, Ramassar V, et al. Identification of a calcium-inducible, cyclosporine sensitive element in the IFN-gamma promoter that is a potential NFAT binding site. Transplantation. 1996;61:933-939[CrossRef][Medline] [Order article via Infotrieve]. 68. Sica A, Dorman L, Viggiano V, et al. Interaction of NF-B and NFAT with the interferon- promoter. J Biol Chem. 1997;272:30412-30420[Abstract/Free Full Text]. 69. Sweetser MT, Hoey T, Sun Y-L, et al. The roles of nuclear factor of activated T cells and ying-yang 1 in activation-induced expression of the interferon- promoter. J Biol Chem. 1998;273:34775-34783[Abstract/Free Full Text]. 70. Kaplan MH, Wurster AL, Grusby MJ. A signal transducer and activator of transcription (Stat)4-independent pathway for the development of T helper type 1 cells. J Exp Med. 1998;188:1191-1196[Abstract/Free Full Text]. 71. Yang J, Murphy TL, Ouyang W, et al. Induction of IFN- production in Th1 CD4+ T cells: evidence for two distinct pathways for promoter activation. Eur J Immunol. 1999;29:548-555[CrossRef][Medline] [Order article via Infotrieve]. 72. Young HA. Regulation of interferon- gene expression. J Interferon Cytokine Res. 1996;16:563-568[Medline] [Order article via Infotrieve]. 73. Ouyang W, Jacobson NG, Bhattarcharya D, et al. The Ets transcription factor ERM is Th1-specific and induced by IL-12 through a Stat4-dependent pathway. Proc Natl Acad Sci U S A. 1999;96:3888-3893[Abstract/Free Full Text]. 74. Penix LA, Weaver WM, Pang Y, et al. Two essential regulatory elements in the human IFN- promoter confer activation specific expression in T cells. J Exp Med. 1993;178:1483-1496[Abstract/Free Full Text]. 75. Penix LA, Sweetser MT, Weaver WM, et al. The proximal regulatory element of the interferon-gamma promoter mediates selective expression in T cells. J Biol Chem. 1996;271:31964-31972[Abstract/Free Full Text]. 76. Aune TM, Penix LA, Rincón MR, et al. Differential transcription directed by discrete gamma interferon promoter elements in naive and memory (effector) CD4 T cells and CD8 T cells. Mol Cell Biol. 1997;17:199-208[Abstract]. 77. Su B, Jacinto E, Hibi M, et al. JNK is involved in signal integration during costimulation of T lymphocytes. Cell. 1994;77:727-736[CrossRef][Medline] [Order article via Infotrieve]. 78. Gupta S, Campbell D, Derijard B, et al. Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science. 1995;267:389-393[Abstract/Free Full Text]. 79. Rincón M, Enslen H, Raingeaud J, et al. Interferon- expression by Th1 effector cells mediated by the p38 MAP kinase signaling pathway. EMBO J. 1998;17:2817-2829[CrossRef][Medline] [Order article via Infotrieve]. 80. Cockerill PN, Shannon MF, Bert AG, et al. The granulocyte-macrophage colony-stimulating factor/interleukin 3 locus is regulated by an inducible cyclosporin A-sensitive enhancer. Proc Natl Acad Sci U S A. 1993;90:2466-2470[Abstract/Free Full Text]. 81. Duncliffe KN, Bert AG, Vadas MA, et al. A T cell-specific enhancer in the interleukin-3 locus is activated cooperatively by Oct and NFAT elements within a DNase I-hypersensitive site. Immunity. 1997;6:175-185[CrossRef][Medline] [Order article via Infotrieve]. 82. Schuh K, Kneitz B, Heyer J, et al. NF-ATp plays a prominent role in the transcriptional induction of Th2-type cytokines. Immunol Lett. 1997;57:171-175[CrossRef][Medline] [Order article via Infotrieve]. 83. Agarwal S, Avni O, Rao A. Cell-type-restricted binding of the transcription factor NFAT to a distal IL-4 enhancer in vivo. Immunity. 2000;12:643-652[CrossRef][Medline] [Order article via Infotrieve]. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. P. Weitzel, M. L. Lesniewski, P. Haviernik, S. Kadereit, P. Leahy, N. J. Greco, and M. J. Laughlin microRNA 184 regulates expression of NFAT1 in umbilical cord blood CD4+ T cells Blood, June 25, 2009; 113(26): 6648 - 6657. [Abstract] [Full Text] [PDF] S. Leung-Theung-Long, I. Mondor, M. Guiraud, C. Lamare, V. Nageleekar, P.-E. Paulet, M. Rincon, and S. Guerder Impaired NFAT Transcriptional Activity in Antigen-Stimulated CD8 T Cells Linked to Defective Phosphorylation of NFAT Transactivation Domain J. Immunol., June 1, 2009; 182(11): 6807 - 6814. [Abstract] [Full Text] [PDF] I. Tassi, M. Cella, R. Presti, A. Colucci, S. Gilfillan, D. R. Littman, and M. Colonna NK cell-activating receptors require PKC-{theta} for sustained signaling, transcriptional activation, and IFN-{gamma} secretion Blood, November 15, 2008; 112(10): 4109 - 4116. [Abstract] [Full Text] [PDF] B. Weigmann, H. A. Lehr, G. Yancopoulos, D. Valenzuela, A. Murphy, S. Stevens, J. Schmidt, P. R. Galle, S. Rose-John, and M. F. Neurath The transcription factor NFATc2 controls IL-6-dependent T cell activation in experimental colitis J. Exp. Med., September 1, 2008; 205(9): 2099 - 2110. [Abstract] [Full Text] [PDF] D. Singh, K. L. McCann, and F. Imani MAPK and heat shock protein 27 activation are associated with respiratory syncytial virus induction of human bronchial epithelial monolayer disruption Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L436 - L445. [Abstract] [Full Text] [PDF] M. Wittmann, C. Killig, M. Bruder, R. Gutzmer, and T. Werfel Critical involvement of IL-12 in IFN-{gamma} induction by calcineurin antagonists in activated human lymphocytes J. Leukoc. Biol., July 1, 2006; 80(1): 75 - 86. [Abstract] [Full Text] [PDF] F. Zhang and M. Boothby T helper type 1-specific Brg1 recruitment and remodeling of nucleosomes positioned at the IFN-{gamma} promoter are Stat4 dependent J. Exp. Med., June 12, 2006; 203(6): 1493 - 1505. [Abstract] [Full Text] [PDF] Y. Gao, J. Tao, M. O. Li, D. Zhang, H. Chi, O. Henegariu, S. M. Kaech, R. J. Davis, R. A. Flavell, and Z. Yin JNK1 Is Essential for CD8+ T Cell-Mediated Tumor Immune Surveillance J. Immunol., November 1, 2005; 175(9): 5783 - 5789. [Abstract] [Full Text] [PDF] L. K. Teixeira, B. P. F. Fonseca, A. Vieira-de-Abreu, B. A. Barboza, B. K. Robbs, P. T. Bozza, and J. P. B. Viola IFN-{gamma} Production by CD8+ T Cells Depends on NFAT1 Transcription Factor and Regulates Th Differentiation J. Immunol., November 1, 2005; 175(9): 5931 - 5939. [Abstract] [Full Text] [PDF] I. Ortega-Perez, E. Cano, F. Were, M. Villar, J. Vazquez, and J. M. Redondo c-Jun N-terminal Kinase (JNK) Positively Regulates NFATc2 Transactivation through Phosphorylation within the N-terminal Regulatory Domain J. Biol. Chem., May 27, 2005; 280(21): 20867 - 20878. [Abstract] [Full Text] [PDF] K.-Y. Chau, A. M. Keane-Myers, M. Fedele, Y. Ikeda, R. J. Creusot, L. Menozzi, D. J. Cousins, G. Manfioletti, L. Feigenbaum, A. Fusco, et al. IFN-{gamma} gene expression is controlled by the architectural transcription factor HMGA1 Int. Immunol., March 1, 2005; 17(3): 297 - 306. [Abstract] [Full Text] [PDF] M. C. Rodriguez-Galan, J. H. Bream, A. Farr, and H. A. Young Synergistic Effect of IL-2, IL-12, and IL-18 on Thymocyte Apoptosis and Th1/Th2 Cytokine Expression J. Immunol., March 1, 2005; 174(5): 2796 - 2804. [Abstract] [Full Text] [PDF] W. S. Park, Y. Bae, D. H. Chung, Y.-L. Choi, B. K. Kim, Y. C. Sung, E. Y. Choi, S. H. Park, and K. C. Jung T cell expression of CIITA represses Th1 immunity Int. Immunol., October 1, 2004; 16(10): 1355 - 1364. [Abstract] [Full Text] [PDF] M. H. A. Roehrl, S. Kang, J. Aramburu, G. Wagner, A. Rao, and P. G. Hogan Selective inhibition of calcineurin-NFAT signaling by blocking protein-protein interaction with small organic molecules PNAS, May 18, 2004; 101(20): 7554 - 7559. [Abstract] [Full Text] [PDF] R. Sancho, A. Macho, L. de La Vega, M. A. Calzado, B. L. Fiebich, G. Appendino, and E. Munoz Immunosuppressive Activity of Endovanilloids: N-Arachidonoyl-Dopamine Inhibits Activation of the NF-{kappa}B, NFAT, and Activator Protein 1 Signaling Pathways J. Immunol., February 15, 2004; 172(4): 2341 - 2351. [Abstract] [Full Text] [PDF] D. U. Lee, O. Avni, L. Chen, and A. Rao A Distal Enhancer in the Interferon-{gamma} (IFN-{gamma}) Locus Revealed by Genome Sequence Comparison J. Biol. Chem., February 6, 2004; 279(6): 4802 - 4810. [Abstract] [Full Text] [PDF] K. J. Erb, T. Twardzik, A. Palmetshofer, G. Wohlleben, U. Tatsch, and E. Serfling Mice Deficient in Nuclear Factor of Activated T-Cell Transcription Factor c2 Mount Increased Th2 Responses after Infection with Nippostrongylus brasiliensis and Decreased Th1 Responses after Mycobacterial Infection Infect. Immun., November 1, 2003; 71(11): 6641 - 6647. [Abstract] [Full Text] [PDF] J. Wang, R. A. Barke, R. Charboneau, H. H. Loh, and S. Roy Morphine Negatively Regulates Interferon-{gamma} Promoter Activity in Activated Murine T Cells through Two Distinct Cyclic AMP-dependent Pathways J. Biol. Chem., September 26, 2003; 278(39): 37622 - 37631. [Abstract] [Full Text] [PDF] J. Yang, G. Hu, S.-W. Wang, Y. Li, R. Martin, K. Li, and Z. Yao Calcineurin/Nuclear Factors of Activated T Cells (NFAT)-activating and Immunoreceptor Tyrosine-based Activation Motif (ITAM)-containing Protein (CNAIP), a Novel ITAM-containing Protein That Activates the Calcineurin/NFAT-signaling Pathway J. Biol. Chem., May 2, 2003; 278(19): 16797 - 16801. [Abstract] [Full Text] [PDF] J. Chen, Y. Amasaki, Y. Kamogawa, M. Nagoya, N. Arai, K.-i. Arai, and S. Miyatake Role of NFATx (NFAT4/NFATc3) in Expression of Immunoregulatory Genes in Murine Peripheral CD4+ T Cells J. Immunol., March 15, 2003; 170(6): 3109 - 3117. [Abstract] [Full Text] [PDF] This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kiani, A. Articles by Rao, A. Search for Related Content PubMed PubMed Citation Articles by Kiani, A. Articles by Rao, A. Related Collections Immunobiology Gene Expression Social Bookmarking                   What's this?

	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020