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NEOPLASIA
From the Department of Genetics and Pathology, Virgen
de la Salud Hospital, Toledo, Spain; the Centro Nacional de
Investigaciones Oncologicas (CN10), Programa de Patologia Molecular,
Madrid, Spain, the Laboratory of Citología Hematológica,
Department of Pathology, Hospital del Mar, Barcelona, Spain; and the
Department of Pathology, Hospital Clinic, Barcelona, Spain.
This study aimed to correlate the frequency of somatic mutations in
the IgVH gene and the use of specific segments
in the VH repertoire with the clinical and
characteristic features of a series of 35 cases of splenic marginal
zone lymphoma (SMZL). The cases were studied by seminested polymerase
chain reaction by using primers from the FR1 and JH region.
The results showed unexpected molecular heterogeneity in this entity,
with 49% unmutated cases (less than 2% somatic mutations). The 7q31
deletions and a shorter overall survival were more frequent in this
group. Additionally a high percentage (18 of 40 sequences) of SMZL
cases showed usage of the VH1-2 segment, thereby
emphasizing the singularity of this neoplasia, suggesting that this
tumor derives from a highly selected B-cell population and encouraging
the search for specific antigens that are pathogenically relevant in
the genesis or progression of this tumor.
(Blood. 2002;99:1299-1304) Splenic marginal zone lymphoma (SMZL), a specific
type of small B-cell lymphoma, is characterized by a peculiar
morphology with micronodular pattern of infiltration, biphasic
cytology, and the almost constant presence of marginal zone
differentiation.1,2 In addition, the tumor is usually
recognized by the presence in peripheral blood of villous lymphocytes
and by a characteristic intrasinusoidal pattern of involvement in bone
marrow biopsies.3,4 Although in most cases SMZL is an
indolent tumor, a small fraction of cases display more aggressive
behavior with peripheral lymph node infiltration, histologic
progression, and eventually death attributable to the
tumor.5
Different observations coincide in pointing toward the relative
molecular heterogeneity of this neoplasia, as observed in the study of
bcl-6 somatic mutation frequency or 7q31-q32
deletions.6-8 Simultaneously, the allegedly marginal zone
origin of this lymphoma has increasingly been questioned. This
hypothesis was based on the almost universal presence of marginal zone
differentiation when splenic sections were examined in this tumor.
However, it was brought into question because the examination of other
organs failed to discover marginal zone differentiation, thus
suggesting that the presence of marginal zone rims was dependent on the
localization of the lesion in the spleen. This suggestion has
subsequently been proven after showing that other lymphoma types, such
as follicular lymphoma, regularly show marginal zone differentiation
when infiltrating the spleen.9
The classification and comprehension of B-cell lymphomas has been aided
greatly by the recognition that B cells within the germinal centers are
exposed to somatic hypermutation of IgVH and
bcl-6,10-12 which in the case of immunoglobulin
genes increases their affinity with the antigens present in the
germinal center microenvironment.13-16 Thus, somatic
mutations of the genes for the variable region of the B-lymphocyte
antigen receptor have proven to be the hallmark of germinal B cells,
and sequence analysis of these genes offered a molecular approach to
identifying the origin of the tumors.17 Thus, most B-cell
non-Hodgkin lymphoma types have been assigned either to hypermutated B
cells18-21 or to naive unmutated B cells, such as mantle
cell lymphoma.22 In B-cell lymphocytic leukemia (B-CLL)
the study of hypermutation in IgVH genes has also revealed
the existence of a strong heterogeneity, an especially relevant finding
here being that unmutated cases show a more aggressive clinical
course.23-26
Although some initial data seem to suggest that SMZL could derive from
mutated postgerminal center B cells, which would be consistent with
their origin in splenic marginal zone B cells (usually showing
hypermutated IgVH sequences), these data were based exclusively on very short series of patients.27-30
Here we analyze a large group of samples corresponding to this
condition and show that roughly half of the cases are characterized by
an unmutated VH sequence with simultaneous loss of 7q and a
more aggressive clinical course. SMZL cases also show a preferential usage of the VH1-2 gene, thus confirming that
involvement of the VH gene is not random in SMZL cases.
To avoid uncertainty in the selection of SMZL cases, we only analyzed
cases that showed a typical histology in their splenectomy specimens,
thereby excluding cases selected exclusively on the basis of peripheral
blood cell morphology.
Tissue samples
These cases were diagnosed on the basis of splenic morphology according
to current criteria.3,31 Small lymphocytic lymphoma, mantle cell lymphoma, follicle center lymphoma, hairy cell leukemia, and lymphoplasmacytoid lymphoma were considered in each case and were
excluded according to morphologic, immunophenotypic, and molecular
findings. Studies for t(11;14) and t(14;18) were performed and were
negative in all cases. The percentage of malignant cells was estimated
by histologic examination and by immunohistochemical CD20 staining, and
at least 70% of cells were tumoral.
Immunohistochemistry
Karyotyping and 7q loss Twenty-two cases were studied for 7q deletion, with cytogenetic and/or loss of heterozygosity (LOH) studies. Cytogenetic studies were performed on lymphoid cells from the spleen in 18 cases according to the methods described by Solé et al.7 In 14 cases, 7q loss was investigated by LOH according to the methods described by Mateo et al.6 These cases have been reported previously.6,7IgVH study Rearranged IgVH genes were amplified by using a seminested polymerase chain reaction (PCR) method as described previously.14 In the first round of PCR, a mixture of 6 framework region 1 (FR1) VH family-specific primers and 2 consensus primers for the JH gene were used. The second round of PCR was performed in 6 separate reactions with one of the 6 VH FR1 primers and JH internal primers.In 4 cases, total RNA was extracted from 5-µm frozen tissue sections, using RNAsy Kit (Qiagen, Hilden, Germany), according to the manufacturer's instructions. Total RNA (1 µg) was used for reverse transcription. Complementary DNA (cDNA) was synthesized by using AMV reverse transcriptase with random primers, following the manufacturer's recommendations. Briefly, DNA 200 ng or cDNA product at a 1/10 dilution was amplified in a volume of 50 µL with 1× PCR buffer, 200 µM dNTPs, 2.5 mM MgCl2, 250 nmol/L of each primer, and 1 U Ampli Taq Gold. In the second round of amplification, the same concentrations of reagent were used, except for MgCl2, which was 1.5 mM. The first-round PCR product (1 µL) was added to the seminested reaction as a template. The PCR conditions of the first round consisted of one cycle at 95°C for 2 minutes, 59°C for 2 minutes, 72°C for 45 seconds followed by 34 cycles at 95°C for 45 seconds, 59°C for 15 seconds, 72°C for 40 seconds, and one final cycle of 72°C for 10 minutes. The second PCR consisted of a total of 25 cycles, using annealing temperatures of 65°C for the VH3 and VH4 primers and 61°C for the VH1, VH2, VH5, and VH6 primers. The denaturing and extension temperatures as well as the cycle conditions were identical to those of the first round of PCR. An aliquot of 10 µL PCR product was visualized in 3% metaphor agarose gel. Sequencing analysis Direct sequencing was performed from both strands by using the same primers as in the amplification. The direct sequencing procedure was performed by using an ABI PRISM 310 or 3700 Genetic Analizers (Applied Biosystems, Weiterstadt, Germany), following the manufacturer's procedure. Mutations were identified by comparison with the germline sequence (Ig BLAST and V BASE sequence directory). In all cases of SMZL the sequencing analysis was confirmed by 2 separate experiments. To determine whether the number of replacement and silent amino acid substitutions identified were indicative of antigen selection, the Chang and Casali method was used.32Statistical analysis A chi square test was used to compare the frequency of patient death because of the tumor between cases with and without IgVH mutations. Survival analyses were performed by using the Kaplan-Meier life-table method. Differences between survival curves were determined according to the log-rank test. P < .05 was required for significance. The SPSS software package (SPSS) was used for all statistical analyses.
Clinical data Of the 35 patients included in the study, 17 (48.6%) were women and 18 (51.4%) were men. Their median age was 64 years (range, 50-75). Thirty-two of the cases were diagnosed at clinical stage IV with bone marrow involvement and 2 at clinical stage I. All patients were treated by splenectomy, and 9 of them also underwent chemotherapy. The mean follow-up of the series was 30 months (range, 3-80). During this period, 12 patients died as a consequence of the tumor.VH gene usage in SMZL We amplified and sequenced 40 clonal IgVH rearrangements in 35 SMZL cases, because 5 cases had 2 different rearrangements. Among the 40 clonal VH gene sequences, 38 were potentially functional and 2 were rendered nonfunctional by out-of-frame rearrangement (stop codon). In 4 of 5 cases, 2 apparently functional VH genes were obtained, which probably represent a lack of allelic exclusion, similar to that described in B-CLL.33Comparison of clonal VH genes with the germline gene
segments revealed that the 40 VH genes used by these 35 cases have a striking bias toward the use of VH1, which was
detected in 20 cases, and more specifically VH1-2, which
was detected in 18 cases (Table 1). In
comparison with the predicted distribution of the VH
repertoire in peripheral blood lymphocytes, in which the use of
VH1 was observed in 19% of CD5+ and
13% of CD5
Mutational analysis In this study, 17 (49%) of 35 cases displayed more than 98% sequence homology with the nearest germline VH gene (unmutated), whereas 18 (51%) of 35 cases showed less than 98% homology with evidence of somatic hypermutation (Tables 2 and 3).
Most of the mutations were localized in FR3 and complementarity-determining region 2 (CDR2; data not shown). The mutations were represented by single nucleotide substitution, and neither point insertion nor deletion was observed. The frequency of mutations was lower in the cases involving the VH1 family (8 of 20), but this frequency was not statistically significant. Interestingly, 2 of 5 cases displaying 2 VH rearrangements
showed a discrepancy in the mutation pattern between 2 rearrangements, one sequence having a high homology with the germinal sequence and the
other showing homology of less than 98%. Of the other 3 cases
with double clonal rearrangement, 2 cases showed both sequences
with 98% or greater homology, and 1 case presented with less than 98%
homology. Messenger RNA analysis confirmed expression in 4 rearrangements; one of the rearrangements was out-of-frame (Table
4).
Analysis of mutation pattern Analysis of distribution of replacement and silent mutations were calculated by considering all possible mutations as described by Chang and Casali.32 Eighteen cases from SMZL showed somatic mutation in the IgVH gene. Evidence for positive selection was shown to be statistically significant in 3 cases (Nos. 253, 371, and 377), whereas the number of replacement mutations in the CDR regions was greater than expected in comparison with the FR regions. In 2 cases (Nos. 202 and 380), evidence for negative selection was observed in which fewer replacement mutations than those expected were seen in the FR regions, indicating pressure to maintain the germinal configuration. In the other 13 cases with somatic mutations, no statistically significant evidence for antigen selection was observed (Table 5).
Correlation between hypermutation frequency and other molecular, immunophenotypical, and clinical findings The frequency of IgVH mutations was compared with the incidence of 7q loss, as determined by karyotyping and LOH. The frequency of 7q loss was higher in the unmutated cases than it was in mutated cases (P = .038; Table 6). No relationship was detected between the frequency of somatic mutation and trisomy 3 or other cytogenetic abnormalities. The expression of CD38 in SMZL cases is shown in Tables 2 and 3. A relationship between the expression of CD38 and IgVH gene mutation status was not observed in this series.
All of the cases analyzed except 4 showed IgD expression. Curiously, in these cases the IgVH gene was mutated (Tables 2 and 3). The presence of a relationship between clinical course and the frequency of somatic mutations was also analyzed. The clinical features of patients with and without somatic mutations are show in Tables 2 and 3. Unmutated cases showed an increased probability of death because of the tumor (P = .022; Table 6). To explore the relationship between tumoral aggressiveness and IgVH status, we selected a group of cases with morphologic or clinical evidence of tumoral progression and compared the frequency of somatic mutations with the other cases. Of the 17 SMZL cases without somatic mutations, 11 (65%) showed either death attributable to the tumor or large cell transformation, whereas similar clinical aggressiveness was found in only 5 (28%) of 18 cases with somatic mutations. This finding is statistically significant (P = .027, Fisher exact test; Table 6). The survival curve was drawn up according to the Kaplan-Meier method.
The graph showing the survival of cases with mutated and unmutated
IgVH genes is shown in Figure
1. Survival at 60 months was 38% for the
unmutated cases and 86% for mutated cases (log-rank test,
P = .049).
This study analyzed mutations in VH genes and the use of the VH gene repertoire in a large number of SMZL cases. To establish beyond any doubt the diagnosis of these cases, examination of splenectomy specimen slides was required as a prior requisite for the inclusion of a case in the series. The results show that 51% of the cases presented less than 98% homology with the germinal sequence. The frequency of unmutated cases (49% in this series) was underestimated in previous analyses, probably because of the small size of the series previously reported and/or the absence of strict morphologic criteria in the recognition of the entity.27-30 Thus, previously published descriptions of somatic IgVH mutations analyzed series with a maximum of 4 to 5 patients, and study of the splenectomy specimen was not formally required for diagnosis. Our analysis also made it possible to confirm the selective use of VH1-2 genes in a high proportion of SMZL cases, as anticipated by Miranda et al.30 The results obtained here are consistent with previously reported data on the frequency of 5' noncoding region bcl-6 gene mutations in SMZL, which already showed the existence of a molecular heterogeneity in this neoplasia.8 This situation is very similar with what has been observed in B-CLL.25 In both conditions a proportion of cases (roughly 50%) are characterized by unmutated sequences, and strikingly in both types of lymphoma these unmutated cases show a more aggressive clinical course. This finding implies that this molecular heterogeneity is related to the clinical characteristics of the tumors in question. The hypothesis that the presence of unmutated VH sequences (as observed in B-CLL, SMZL, and mantle cell lymphoma) could be used as a reliable marker of aggressive behavior in small B-cell lymphomas needs to be properly addressed in larger series of patients, but it could justify a role for molecular analysis in the selection of therapy. The possibility of molecular heterogeneity in this neoplasia is strengthened because 7q deletions are significantly more frequent in unmutated cases. The presence of a selective 7q22-q32 deletion has been claimed to be the most frequent abnormality in this neoplasia,6,7 and it is also associated with a more aggressive clinical course. The selective use of the VH1-2 gene in a high proportion of cases also suggests that the origin of this lymphoma could be related to specific subsets of B lymphocytes, primed for its growth by autoantigens or superantigens. Although the clinical reports of a small proportion of SMZL patients have revealed autoimmune phenomena before the open development of the tumor, such as hemolytic autoimmune anemia or autoimmune thrombocytopenia, this situation does not apply to a high enough proportion of these patients to justify the selective use of the VH1-2 gene. It is not known how this finding of preferential VH gene involvement relates to tumor genesis. One possibility is that a common antigen could be involved in the preferential usage of individual VH genes, causing the expansion of certain lymphocyte clones, and that malignant transformation occurs within the expanded clone. A restricted involvement of IgVH segments has also been described in B-CLL, in which VH1-69 is involved in up to 12% of cases,23,25 or some mucosa-associated lymphoid tissue lymphomas, such as salivary gland lymphomas35 (8 of 14 cases), and also in hepatitis C virus-associated lymphoplasmacytic lymphomas.36 In all of these cases a selective involvement of VH1-69 has been observed. This involvement contrasts with the more frequent situation of follicular lymphoma, large B-cell lymphoma, or Burkitt lymphoma in which the expression of the VH3 or VH4 gene family is more common, thus reflecting more closely the repertoire observed in normal peripheral blood lymphocytes.19,21,34,37 The selective involvement of VH1-2 emphasizes the singularity of this neoplasia, suggesting that this tumor derives from a highly selected B-cell population. This involvement makes it advisable to search for specific antigens pathogenically relevant in the genesis or progression of this tumor. Nevertheless, the Chang and Casalli32 method showed evidence of positive antigenic selection in only 3 of 18 cases, which introduces some caveats about the potential role of antigens in the genesis of this tumoral type. A relationship between the frequency of somatic mutations and IgVH has been described in peripheral blood lymphocytes in which lymphocytes expressing VH1 family genes have a lower frequency of mutation in comparison with the higher frequency observed for VH3 or VH4.34 This frequency is reproduced in B-CLL in which the use of VH1 is accompanied by a frequency of 17%, opposed to a frequency of 90% among lymphocytes with VH3. Nevertheless, in SMZL this relationship is not detected so clearly; thus, the frequency of mutations for cases expressing VH1 was 40%, 60% for VH3, and 50% in lymphomas expressing VH4, which could be interpreted as supporting the hypothesis that, at least in this entity, heterogeneity in the frequency of somatic mutation is not clearly dependent on the selective involvement of VH1 genes. A striking finding in this series is the presence of 5 cases with double IgH rearrangements. Although there are a range of possible interpretations of this observation (bystander B-cell clones, lack of allelic exclusion, or presence of more than one tumoral clone), a similar situation in B-CLL has been linked to lack of allelic exclusion.33 Nevertheless, this situation has not been demonstrated in SMZL and needs to be properly investigated. These data once again raise doubts about the proposed cell origin of this lymphoma type in the splenic marginal zone, because marginal zone B cells in the spleen have been shown to carry mutated VH sequences. Instead, they support the hypothesis that at least a significant fraction of cases could derive from naive cells not exposed to antigen B cells situated in the mantle zone.
We thank Dr A. I. Sáez for her useful help with statistical analysis. Drs T. Flores, T. Alvaro, P. Gonzalvo, L. Bernardó, M. Medina, V. Lescano, A. Santana, and Alvarez from the Pathology Departments at hospitals in Salamanca, Tortosa, Jarrió, Gerona, Osuna, Plasencia, Madrid, and Baracaldo (Spain) for kindly providing cases included in this series.
Submitted June 22, 2001; accepted August 20, 2001.
Supported by grant FIS 99/0705, 01/0035, from the Fondo de Investigaciones Sanitarias, Ministerio de Sanidad y Consumo and grant 1FD97-0431 from the Comisión Interministerial de Ciencia y Tecnología, Spain.
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked "advertisement" in accordance with 18 U.S.C. section 1734.
Reprints: Miguel A. Piris, Centro Nacional de Investigaciones Oncológicas, Programa de Patología Molecular, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo Km 2, 28220 Majadahonda-Madrid, Spain; e-mail: mapiris{at}cnio.es.
1. Mollejo M, Lloret E, Menarquez J, Piris MA. Lymph node involvement by splenic marginal zone lymphoma morphological an immunohistochemical features. Am J Pathol. 1997;21:772-780. 2. Isaacson PG, Piris MA. Splenic marginal zone lymphoma. Adv Anat Pathol. 1997;4:191-201.
3.
Isaacson PG, Matutes E, Burke M, Catovsky D.
The histopathology of splenic marginal lymphoma with villous lymphocytes.
Blood.
1994;84:3828-3834 4. Franco V, Florena AM, Campesi G. Intrasinusoidal bone marrow infiltration: a possible hallmark of splenic lymphoma. Histopathology. 1996;29:571-575[CrossRef][Medline] [Order article via Infotrieve]. 5. Lloret E, Mollejo M, Mateo MS, et al. Splenic marginal zone lymphoma with increased number of blasts: an aggressive variant? Hum Pathol. 1999;30:1153-1160[CrossRef][Medline] [Order article via Infotrieve].
6.
Mateo M, Mollejo M, Villuendas R, et al.
7q31-32 allelic loss is a frequent finding in splenic marginal zone lymphoma.
Am J Pathol.
1999;154:1583-1589 7. Sole F, Salido M, Espinet B, et al. Splenic marginal zone B-cell lymphomas: two cytogenetic subtypes, one with gain of 3q and the other with loss of 7q. Haematologica. 2001;86:71-77[Medline] [Order article via Infotrieve]. 8. Mateo MS, Mollejo M, Villuendas R, et al. Molecular heterogeneity of splenic marginal zone lymphomas: analysis of mutations in the 5' non-coding region of the bcl-6 gene. Leukemia. 2001;15:628-634[CrossRef][Medline] [Order article via Infotrieve]. 9. Piris MA, Mollejo M, Campo E, Menarguez J, Flores T, Isaacson PG. A marginal zone pattern may be found in different varieties of non-Hodgkin's lymphoma: the morphology and immunohistology of splenic involvement by B-cell lymphomas simulating splenic marginal zone lymphoma. Histopathology. 1998;33:230-239[CrossRef][Medline] [Order article via Infotrieve]. 10. Hummel M, Stein H. Clinical relevance of immunoglobulin mutation analysis. Curr Opin Oncol. 2000;12:395-402[CrossRef][Medline] [Order article via Infotrieve]. 11. Shen HM, Peters A, Baron B, Zhu X, Storb U. Mutations of BCL-6 gene in normal B cells by the process of somatic hypermutation of Ig genes. Science. 1998;280:1751-1752.
12.
Pasqualucci L, Migliazza A, Fracchiola N, et al.
BCL-6 mutations in normal germinal centre B cells: evidence of somatic hypermutation acting outside Ig loci.
Proc Natl Acad Sci U S A.
1998;95:11816-11821 13. Berek C, Berger A, Apel M. Maturation of the immune response in germinal centres. Cell. 1991;67:1121-1129[CrossRef][Medline] [Order article via Infotrieve]. 14. Küppers R, Zhao M, Hansmann ML, Rayewsky K. Tracing B cell development in human germinal centres by molecular analysis of single cells picked from histological sections. EMBO J. 1993;12:4955-4967[Medline] [Order article via Infotrieve].
15.
Pascual V, Liu Y-J, Magalski A, Bouteiller O, Banchereau J, Capra JD.
Analysis of somatic mutation in five B cell subsets of human tonsil.
J Exp Med.
1994;180:329-339
16.
Klein U, Kuppers R, Rajewsky K.
Evidence for a large compartment of IgM-expressing memory B cells in humans.
Blood.
1997;89:1288-1298
17.
Kuppers R, Klein U, Hansmann ML, Rajewsky K.
Cellular origin of human B-cell lymphomas.
N Engl J Med.
1999;341:1520-1529 18. Stamatopoulos K, Kosmas C, Belessi C, Stavroyianni N, Kyriazopoulos P, Papadaki T. Molecular insights into the immunopathogenesis of follicular lymphoma. Immunol Today. 2000;21:298-305[CrossRef][Medline] [Order article via Infotrieve].
19.
Lossos IS, Okada CY, Tibshirani R, et al.
Molecular analysis of immunoglobulin genes in diffuse large B-cell lymphomas.
Blood.
2000;95:1797-1803 20. Vescio RA, Cao J, Hong CH, et al. Myeloma Ig heavy chain V region sequences reveal prior antigenic selection and marked somatic mutation but no intraclonal diversity. J Immunol. 1995;155:2487-2497[Abstract].
21.
Chapman CJ, Mockridge CI, Rowe M, Rickinson AB, Stevenson FK.
Analysis of VH genes used by neoplastic B cells in endemic Burkitt's lymphoma shows somatic hypermutation and intraclonal heterogeneity.
Blood.
1995;85:2176-2181
22.
Hummel M, Tamaru J, Kalvelage B, Stein H.
Mantle cell (previously centrocytic) lymphomas express VH genes with no or very little somatic mutations like the physiologic cells of the follicle mantle.
Blood.
1994;84:403-407 23. Fais F, Ghiotto F, Hashimoto S, et al. Chronic lymphocytic leukemia B cells express restricted sets of mutated and unmutated antigen receptors. J Clin Invest. 1998;102:1515-1525[Medline] [Order article via Infotrieve].
24.
Naylor M, Capra JD.
Mutational status of IgVH genes provides clinically valuable information in B-cell chronic lymphocytic leukemia.
Blood.
1999;94:1837-1839
25.
Hamblin TJ, Davis Z, Gardiner A, Oscier DG, Stevenson FK.
Unmutated IgVH genes are associated with a more aggressive form of chronic lymphocytic leukemia.
Blood.
1999;94:1848-1854
26.
Damle RN, Wasil T, Fais F, et al.
IgV gene mutation status and CD38 expression as novel prognostic indicators in chronic lymphocytic leukemia.
Blood.
1999;94:1840-1847
27.
Zhu D, Oscier DG, Stevenson FK.
Splenic lymphoma with lymphocytes involves B cells with extensively mutated Ig heavy chain variable region genes.
Blood.
1995;85:1603-1607 28. Dunn-Walters DK, Boursier L, Spencer J, Isaacson PG. Analysis of immunoglobulin genes in splenic marginal zone lymphoma suggests ongoing mutation. Human Pathol. 1998;29:585-593[CrossRef][Medline] [Order article via Infotrieve].
29.
Tierens A, Delabie J, Pittaluga S, Driessen A, Wolf-Peeters CD.
Mutation analysis of the rearranged immunoglobulin heavy chain genes of marginal zone cell lymphomas indicates an origin from different marginal zone B lymphocyte subsets.
Blood.
1998;91:2381-2386 30. Miranda RN, Cousr JB, Hammer RD, Collins RD, Vnencak-Jones CL. Somatic mutation analysis of IgH variable regions reveals that tumor cells of most parafollicular (monocytoid) B-cell lymphoma, splenic marginal zone B-cell lymphoma, and some hairy cell leukaemia are composed of memory B lymphocytes. Human Pathol. 1999;30:306-312[CrossRef][Medline] [Order article via Infotrieve]. 31. Mollejo M, Menarguez J, Lloret E, et al. Splenic marginal zone lymphoma, a distinctive type of low-grade B-cell lymphoma: a clinicopathological study of 13 cases. Am J Surg Pathol. 1995;19:1146-1157[Medline] [Order article via Infotrieve]. 32. Chang B, Casali P. The CDR1 sequences of a major proportion of human germline IgVH genes are inherently susceptible to amino acid replacement. Immunol Today. 1994;15:367-373[CrossRef][Medline] [Order article via Infotrieve].
33.
Rassenti LZ, Kipps TJ.
Lack of allelic exclusion in B cell chronic lymphocytic leukemia.
J Exp Med.
1997;185:1435-1445
34.
Brezinschek HP, Foster SJ, Brezinschek RI, Dorner T, Domiati-Saad R, Lipsky PE.
Analysis of the human VH gene repertoire: differential effects of selection and somatic hypermutation on human peripheral CD5(+)/IgM+ and CD5(
35.
Miklos JA, Swerdlow SH, Bahler DW.
Salivary gland mucosa-associated lymphoid tissue lymphoma immunoglobulin V(H) genes show frequent use of V1-69 with distinctive CDR3 features.
Blood.
2000;95:3878-3884
36.
Chan CH, Hadlock KG, Foung SK, Levy S.
V(H)1-69 gene is preferentially used by hepatitis C virus-associated B cell lymphomas and by normal B cells responding to the E2 viral antigen.
Blood.
2000;97:1023-1026 37. Noppe SM, Heirman C, Bakkus MH, Brissinck J, Schots R, Thielemans K. The genetic variability of the VH genes in follicular lymphoma: the impact of the hypermutation mechanism. Br J Haematol. 1999;107:625-640[CrossRef][Medline] [Order article via Infotrieve].
© 2002 by The American Society of Hematology.
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  C. KALPADAKIS, G. A. PANGALIS, E. DIMITRIADOU, M. K. ANGELOPOULOU, M. P. SIAKANTARIS, M.-C. KYRTSONIS, M. XIMERIS, T. TZENOU, S. SAHANAS, X. YIAKOUMIS, et al. Mutation Analysis of IgVH Genes in Splenic Marginal Zone Lymphomas: Correlation with Clinical Characteristics and Outcome Anticancer Res, May 1, 2009; 29(5): 1811 - 1816. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Abramson, M. Chatterji, and A. Rahemtullah Case 39-2008 -- A 51-Year-Old Woman with Splenomegaly and Anemia N. Engl. J. Med., December 18, 2008; 359(25): 2707 - 2718. [Full Text] [PDF]
|
|
|
|
 |
|
 L. Tracey, M. Aggarwal, M. Garcia-Cosio, R. Villuendas, P. Algara, M. Sanchez-Beato, A. Sanchez-Aguilera, J. F. Garcia, A. Rodriguez, F. I. Camacho, et al. Somatic hypermutation signature in B-cell low-grade lymphomas Haematologica, August 1, 2008; 93(8): 1186 - 1194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Traverse-Glehen, L. Baseggio, E. C. Bauchu, D. Morel, S. Gazzo, M. Ffrench, A. Verney, D. Rolland, C. Thieblemont, J.-P. Magaud, et al. Splenic red pulp lymphoma with numerous basophilic villous lymphocytes: a distinct clinicopathologic and molecular entity? Blood, February 15, 2008; 111(4): 2253 - 2260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kahl and D. Yang Marginal Zone Lymphomas: Management of Nodal, Splenic, and MALT NHL Hematology, January 1, 2008; 2008(1): 359 - 364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Martin-Jimenez, R. Garcia-Sanz, A. Balanzategui, M. Alcoceba, E. Ocio, M{a} L. Sanchez, M. Gonzalez, and J. San Miguel Molecular characterization of heavy chain immunoglobulin gene rearrangements in Waldenstrom's macroglobulinemia and IgM monoclonal gammopathy of undetermined significance Haematologica, May 1, 2007; 92(5): 635 - 642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Stamatopoulos, C. Belessi, C. Moreno, M. Boudjograh, G. Guida, T. Smilevska, L. Belhoul, S. Stella, N. Stavroyianni, M. Crespo, et al. Over 20% of patients with chronic lymphocytic leukemia carry stereotyped receptors: pathogenetic implications and clinical correlations Blood, January 1, 2007; 109(1): 259 - 270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Arcaini, M. Lazzarino, N. Colombo, S. Burcheri, E. Boveri, M. Paulli, E. Morra, M. Gambacorta, S. Cortelazzo, A. Tucci, et al. Splenic marginal zone lymphoma: a prognostic model for clinical use Blood, June 15, 2006; 107(12): 4643 - 4649. [Abstract] [Full Text] [PDF]
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E. Ruiz-Ballesteros, M. Mollejo, A. Rodriguez, F. I. Camacho, P. Algara, N. Martinez, M. Pollan, A. Sanchez-Aguilera, J. Menarguez, E. Campo, et al. Splenic marginal zone lymphoma: proposal of new diagnostic and prognostic markers identified after tissue and cDNA microarray analysis Blood, September 1, 2005; 106(5): 1831 - 1838. [Abstract] [Full Text] [PDF]
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 G. Troen, V. Nygaard, T.-K. Jenssen, I. M. Ikonomou, A. Tierens, E. Matutes, A. Gruszka-Westwood, D. Catovsky, O. Myklebost, G. Lauritzsen, et al. Constitutive Expression of the AP-1 Transcription Factors c-jun, junD, junB, and c-fos and the Marginal Zone B-Cell Transcription Factor Notch2 in Splenic Marginal Zone Lymphoma J. Mol. Diagn., November 1, 2004; 6(4): 297 - 307. [Abstract] [Full Text] [PDF]
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 A. Rodriguez, N. Martinez, F. I. Camacho, E. Ruiz-Ballesteros, P. Algara, J.-F. Garcia, J. Menarguez, T. Alvaro, M. F. Fresno, F. Solano, et al. Variability in the Degree of Expression of Phosphorylated I{kappa}B{alpha} in Chronic Lymphocytic Leukemia Cases With Nodal Involvement Clin. Cancer Res., October 15, 2004; 10(20): 6796 - 6806. [Abstract] [Full Text] [PDF]
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 M. P. Cancro and J. F. Kearney B Cell Positive Selection: Road Map to the Primary Repertoire? J. Immunol., July 1, 2004; 173(1): 15 - 19. [Abstract] [Full Text] [PDF]
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 S. A. Pileri, P. L. Zinzani, P. Went, A. Pileri Jr, and M. Bendandi Indolent lymphoma: the pathologist's viewpoint Ann. Onc., January 1, 2004; 15(1): 12 - 18. [Abstract] [Full Text] [PDF]
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 N. Martinez, F. I. Camacho, P. Algara, A. Rodriguez, A. Dopazo, E. Ruiz-Ballesteros, P. Martin, J. A. Martinez-Climent, J. Garcia-Conde, J. Menarguez, et al. The Molecular Signature of Mantle Cell Lymphoma Reveals Multiple Signals Favoring Cell Survival Cancer Res., December 1, 2003; 63(23): 8226 - 8232. [Abstract] [Full Text] [PDF]
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J. Orchard, R. Garand, Z. Davis, G. Babbage, S. Sahota, E. Matutes, D. Catovsky, P. W. Thomas, H. Avet-Loiseau, and D. Oscier A subset of t(11;14) lymphoma with mantle cell features displays mutated IgVH genes and includes patients with good prognosis, nonnodal disease Blood, June 15, 2003; 101(12): 4975 - 4981. [Abstract] [Full Text] [PDF]
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F. I. Camacho, P. Algara, A. Rodriguez, E. Ruiz-Ballesteros, M. Mollejo, N. Martinez, J. A. Martinez-Climent, M. Gonzalez, M. Mateo, A. Caleo, et al. Molecular heterogeneity in MCL defined by the use of specific VH genes and the frequency of somatic mutations Blood, May 15, 2003; 101(10): 4042 - 4046. [Abstract] [Full Text] [PDF]
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V. Franco, A. M. Florena, and E. Iannitto Splenic marginal zone lymphoma Blood, April 1, 2003; 101(7): 2464 - 2472. [Abstract] [Full Text] [PDF]
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 A. Tierens, J. Delabie, A. Malecka, J. Wang, A. Gruszka-Westwood, D. Catovsky, and E. Matutes Splenic Marginal Zone Lymphoma with Villous Lymphocytes Shows On-Going Immunoglobulin Gene Mutations Am. J. Pathol., February 1, 2003; 162(2): 681 - 689. [Abstract] [Full Text] [PDF]
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J. I. Chacon, M. Mollejo, E. Munoz, P. Algara, M. Mateo, L. Lopez, J. Andrade, I. G. Carbonero, B. Martinez, M. A. Piris, et al. Splenic marginal zone lymphoma: clinical characteristics and prognostic factors in a series of 60 patients Blood, August 13, 2002; 100(5): 1648 - 1654. [Abstract] [Full Text] [PDF]
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D. W. Bahler, J. A. Pindzola, and S. H. Swerdlow Splenic Marginal Zone Lymphomas Appear to Originate from Different B Cell Types Am. J. Pathol., July 1, 2002; 161(1): 81 - 88. [Abstract] [Full Text] [PDF]
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Top
Abstract
Introduction
, and 
(DAKO), as well as CD5 and CD10 (Novocastra, Newcastle, United Kingdom).
cells, VH3 is usually observed in
54% to 56% of cells. This finding shows a statistically significant
difference (P < .000 01).
)/IgM+ B cells.
J Clin Invest.
1997;99:2488-2501