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CLINICAL OBSERVATIONS, INTERVENTIONS, AND THERAPEUTIC TRIALS
From the Division of Oncology, Department of Medicine,
and the Stanford Blood Center, Stanford University School of Medicine,
CA.
Tumor-specific clonal immunoglobulin expressed by B-cell lymphomas
(idiotype [Id]) can serve as a target for active immunotherapy. We
have previously described the vaccination of 4 patients with follicular
lymphoma using dendritic cells (DCs) pulsed with tumor-derived Id
protein and now report on 35 patients treated using this approach. Among 10 initial patients with measurable lymphoma, 8 mounted T-cell
proliferative anti-Id responses, and 4 had clinical responses Low-grade follicular B-cell non-Hodgkin lymphoma
(NHL) is an incurable disease characterized by relatively slow growth
and excellent initial responsiveness to chemotherapy but also by a continuous pattern of relapse and death.1 Despite recent
advances in chemotherapy, radiation therapy, and supportive care,
overall survival for low-grade NHL has not significantly improved in
the past 3 decades. This fact, coupled with the substantial toxicities of standard treatments, has fueled the search for novel and more tumor-selective therapies.
Fortunately, there is a target expressed by B-cell lymphomas that can
be exploited using an active immunotherapeutic approach. This target is
the idiotype (Id), which is composed of the unique antigenic
determinants in the variable regions of the clonal immunoglobulin (Ig)
expressed by the tumor cells. Each normal B cell expresses an
immunoglobulin with unique variable region sequences in the heavy and
light chains that together form the antigen-binding site. When a B cell
undergoes malignant transformation, these sequences are maintained by
the malignant clone and can thus serve as a tumor-specific antigen. Id
proteins contain structures that can be recognized by
antibodies2-5 and by CD4+ T
cells5-8 and CD8+ T cells9-12 and
can be isolated from autologous tumor cells and formulated into a
custom-made vaccine.
In preclinical studies, vaccination of mice with Id protein coupled to
the highly immunogenic carrier protein keyhole limpet hemocyanin
(Id-KLH) has been shown to protect animals from subsequent tumor
challenge5,13-16 and to cure established lymphoma when combined with chemotherapy.16,17 In our previous clinical
studies, vaccination with Id-KLH plus a chemical adjuvant was found to induce anti-Id immune responses in approximately half the patients with
follicular NHL. These immune responses were correlated with improved
disease-free survival and overall survival.6,7
Subsequently, ways have been sought to improve the frequency and the
potency of anti-Id immune responses in patients with NHL.
The critical role played by dentritic cells (DCs) in the initiation of
immune responses makes them an attractive addition to cancer vaccine
strategies.18,19 When pulsed (cocultured) with
tumor-derived peptides or proteins or when transduced with tumor
antigen-encoding viruses or nucleic acids and administered as a
cellular vaccine, DCs have been shown to promote protective and even
therapeutic antitumor immunity in murine tumor models. These studies
have provided a compelling rationale for the clinical use of DCs in
active vaccination strategies against human cancer.20 Based on studies in a murine lymphoma model showing that vaccination with Id-pulsed DCs could induce protective antitumor
immunity,21 we initiated a pilot clinical trial in 1993 of
Id-pulsed DC vaccination for patients with follicular NHL. In the
preliminary report of our first 4 patients,22 we described
the induction of cellular anti-Id responses and evidence of antitumor
effects in 3 of 4 patients. Since our initial report, clinical activity
of DC-based vaccines has also been demonstrated in
melanoma,23,24 prostate carcinoma,25,26 renal
cell carcinoma,27 and carcinoembryonic antigen-expressing
cancers.28
We now report on our expanded experience and long-term follow-up of 35 patients with follicular NHL treated with Id-pulsed DC vaccination,
including a cohort of 25 patients treated in first remission after
their initial cytoreductive chemotherapy. Our observations include
durable, objective tumor regression in patients with measurable
disease, induction of T-cell anti-Id immune responses, and induction of
anti-Id antibodies capable of specifically recognizing and mediating
signal transduction in autologous tumor cells. In addition, we found
that patients who were resistant to or who had relapses after primary
Id-pulsed DC vaccination could achieve complete tumor regression after
the administration of booster vaccinations of Id-KLH protein. These
findings confirm the clinical activity of Id-pulsed DCs and Id-KLH
vaccination in follicular NHL and provide the rationale for applying
similar strategies to other B-cell malignancies.
Patients
Vaccine production
We used peripheral blood DCs for our Id-pulsed DC vaccine approach given the proven capacity of these cells to prime naive CD4+ and CD8+ T cells in vitro.32,33 DCs were isolated from leukapheresis products by a series of density gradient centrifugation steps, as previously described.22 Peripheral blood mononuclear cells (PBMCs) were obtained by leukapheresis using a COBE cell separator apparatus followed by Ficoll-Hypaque sedimentation (Pharmacia, Uppsala, Sweden). Monocytes were then removed by a discontinuous (50%) Percoll gradient (Pharmacia). The resultant high-density fraction was cultured overnight in RPMI 1640 plus 10% human AB serum in Teflon vessels (Savillex, Minneapolis, MN) along with antigen at 2 µg/mL. During this period, DC precursors spontaneously matured without exogenous cytokines and acquired the low-density characteristic of DCs. The next day, high-density lymphocytes were removed using a 15% metrizamide gradient (Sigma, St Louis, MO), and the low-density, DC-enriched fraction was then recultured overnight in antigen at 50 µg/mL and was harvested, washed, and resuspended in sterile saline for re-infusion. The choice of antigen concentrations for sequential overnight incubations with DCs was based on our earlier in vitro studies using nominal antigens32,33 and on the limited availability of Id proteins. DCs were enumerated by flow cytometry as lineage (CD3, CD14, CD19) negative and HLA-DR bright, and purity was confirmed by morphology. For patients D1 to D22, DCs were divided and pulsed separately with Id and KLH as in our original report,22 but patients D23 to D35 received DCs pulsed with Id-KLH conjugate as indicated in Table 2. Vaccine treatments Antigen-pulsed DCs were administered intravenously after premedication with acetaminophen and diphenhydramine. Patients received 3 monthly infusions, followed by a fourth given 2 to 6 months later. Each infusion was followed 2 weeks later by subcutaneous injections of 0.5 mg soluble Id and KLH proteins (without DCs). In patients D1 to D22, Id and KLH proteins were injected separately at different sites as in our original report,22 whereas in patients D23 to D35, Id-KLH conjugate was administered at a single site. Where indicated (Table 3), certain patients received further subcutaneous booster vaccinations consisting of 0.5 mg Id protein coupled to 0.5 mg KLH and emulsified in the chemical adjuvant SAF1 as previously described.6,7 Five of these vaccinations were administered at weeks 0, 4, 8, 12, and 20.
Cellular immune response assessments T-cell proliferation assays were performed as reported previously.6,7,22 Fresh PBMCs were cultured in quadruplicate in media alone or with tumor Id, irrelevant Id proteins, or KLH at 0.1, 1.0, 10, and 100 µg/mL. Incorporation of [3H]-thymidine (Amersham Pharmacia Biotech, Piscataway, NJ; 185 GBq/mmol) was measured after an overnight pulse (1 µCi [0.037 MBq]/well) on day 5. A response was interpreted as positive when the incorporation of more than twice the background (media alone) with at least one antigen concentration was observed on 2 or more occasions.Tumor-specific cytotoxicity assays for patient D12 were adapted from
methods described by Schultze et al.34 Autologous
follicular lymphoma cells were activated by 3- to 5-day coculture with
irradiated (55 Gy) CD40L-expressing mouse L cells (a gift of Yong-Jun
Liu, DNAX, Palo Alto, CA). Activated tumor cells were then irradiated (64 Gy) and were used to restimulate cryopreserved prevaccine and
postvaccine (vaccine course 3) PBMCs for 2 weekly cycles. Interleukin-2
(Chiron, Emeryville, CA) was added at 30 IU/mL on the third day of
culture, with fresh medium added every 3 days thereafter. On day 14, cytotoxicity against unmodified, cryopreserved autologous follicular
lymphoma cells, irrelevant tumor cells (non-HLA matched follicular
lymphoma), and normal B cells was assessed in a standard 4-hour
chromium Cr 51 release assay.12 Normal autologous
peripheral blood B cells were isolated by negative selection using the
RosetteSep reagent (StemCell Technologies, Vancouver, BC, Canada), and
flow cytometry showed that they were more than 95% CD19+
and without evidence of light chain restriction (data not shown). Target cells were labeled with 51Cr (Amersham, 150 µCi
(555 MBq/mL) per 106 target cells) for 1.5 to 2 hours and
were washed 3 times, and 10 000 targets were incubated with effector
cells at the effector-target (E/T) ratios of 100, 30, 10, and 3. Unlabeled K562 erythroleukemia cells were added at a ratio of 50:1 to
labeled targets to quench natural killer cell-mediated lysis as
previously described.12 Spontaneous release of
51Cr was less than 20% for all target cell populations.
The percentage specific lysis is expressed as the mean of
triplicate values, determined using the equation: % Specific
lysis = (experimental cpm Humoral immune response assessments Enzyme-linked immunosorbent assay was used to analyze sera of all patients for anti-Id antibodies. Autologous IgM tumor Id proteins or irrelevant IgM Id proteins were captured onto microtiter plates coated with goat anti-human IgM (Biosource International, Camarillo, CA). When the tumor Id was an IgG, the plates were coated directly either with F(ab')2 fragments of the IgG Id produced by digestion with immobilized pepsin (Pierce, Rockford, IL) or with whole IgG Id. Prevaccine and postvaccine sera were serially diluted and allowed to bind to the target Id proteins. Reagents used to detect bound antibodies depended on the isotype of the target Id molecule. When the Id protein was an IgM or a F(ab')2 fragment of IgG, bound anti-Id antibodies of the IgG subclass were detected using polyclonal goat anti-human IgG coupled to horseradish peroxidase (HRP; Southern Biotech, Birmingham, AL). When the target Id protein was whole IgG, bound anti-Id antibodies were detected with polyclonal anti-human IgM HRP or with antibodies to the immunoglobulin light chain ( or
) opposite that of the tumor Id. A response was considered positive
when a 4-fold increase in anti-Id titer was found compared to the
prevaccine serum and the isotype-matched irrelevant Id proteins used as
specificity controls. Antibody responses to KLH were measured as
previously described.7 For tumor cell staining
experiments, cryopreserved IgM-positive tumor cells were incubated with
1% prevaccine or postvaccine serum overnight at 4°C, washed, and
incubated with goat anti-human IgG F(ab')2-phycoerythrin
(PE) followed by counter-staining with anti-human IgM
F(ab')2-fluorescein isothiocyanate (FITC; Biosource).
Stained cells were analyzed using a FACScan flow cytometer (Becton
Dickinson, San Jose, CA).
Analysis of signal transduction in tumor cells Cryopreserved tumor cells (2 × 106/mL) were incubated with 1% relevant or irrelevant prevaccine or postvaccine serum for 3 hours on ice to allow the binding of anti-Id antibodies to the cell surface, warmed to 37°C for 2 minutes, and immediately chilled by the addition of ice-cold phosphate-buffered saline plus 1 mM Na3VO4 and washed once. Goat F(ab')2 anti-µm antibodies (Biosource) served as a positive control. Western blotting was performed as previously described.35 Briefly, aliquots of cells treated as above were lysed, and solubilized proteins were separated by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions and were transferred to nitrocellulose (Schleicher and Schuell, Keene, NJ). The blot was then stained with antiphosphotyrosine antibody 4G10 (Upstate Biotechnology, Lake Placid, NY) followed by goat anti-mouse IgG-HRP (Southern Biotech) and was developed using the enhanced chemiluminescence reagent (Amersham Pharmacia Biotech). For the detection of tyrosine phosphorylation by flow cytometry, cells treated as above were fixed and permeabilized (FACS Lysing Solution; Becton Dickinson), then were stained with antibody 4G10 coupled to FITC (Upstate Biotechnology). Aliquots of cells counter-stained with phycoerythrin-labeled anti-CD20 or anti-CD3 antibodies were fixed in 1% paraformaldehyde, and phosphotyrosine content was quantitated by FACScan.
Pilot experience in patients with measurable follicular lymphoma Idiotype-pulsed DC vaccination was first applied in the pilot phase of our study to 10 patients with measurable relapsed or residual tumor after chemotherapy. Characteristics of these patients are shown in Table 1. In this group, native, unmodified Id protein was used for pulsing the DCs. Patients D1 to D4 were described in our initial report.22 Eight of the 10 patients underwent 2 or more chemotherapy regimens before vaccination, and all but one had multiple sites of evaluable tumor. Total numbers of DCs administered over the 4 infusions were dictated by cell yield and ranged from 12 to 69 × 106 (median, 19 × 106; range, 2-32 × 106/infusion). Side effects (mild to moderate chills, rigors, or fevers) were associated with less than 20% of infusions and were self-limiting.After vaccination, T-cell proliferative responses to Id protein developed in 8 of the 10 patients. Serum anti-Id antibodies of the IgG class were not found in any patient using our traditional anti-IgG detection strategy,6,7 but 2 patients (D3 and D8) were found to have serum anti-Id antibodies of the IgM class after vaccination. All 10 patients mounted humoral and cellular proliferative responses to KLH. Evidence of antitumor activity was seen in 4 of the 10 patients,
including 2 who experienced CR. Complete regression of paracardiac and
mediastinal lymph nodes occurred in patient D1.22 This
patient's remission lasted 44 months, after which recurrent disease
was confined almost exclusively to bone, while all known prior sites of
disease were spared. Patient D9 experienced complete regression of
axillary (Figure 1), peri-aortic, and
iliac lymph nodes. This patient's CR lasted 57 months, when recurrent
disease was detected in a single previously uninvolved site (left
thyroid). Patient D2 had a PR of peri-aortic and para-iliac nodes
lasting 12 months. In patient D3, the only evidence of disease at the
time of vaccination was a tumor-specific PCR signal in the bone marrow
that became negative after vaccination,22 and she has
remained without clinical evidence of disease progression for more than
6 years. Of the 4 patients with clinical responses, 3 of 4 had T-cell
anti-Id immune responses, and patient D9 had no detectable in vitro
anti-Id response despite having achieved durable CR. There was no
correlation of immune or clinical responses to the number of DCs
infused. Follow-up status of the 10 patients is listed in Table 1. All 10 patients remain alive at a median follow-up of 64 months after vaccination (range, 47-94 months) and 103 months after diagnosis (range, 81-276 months). Patient D3 has not received any subsequent treatments, and the remaining patients have undergone additional treatments as indicated. Patient D8 remains in CR after booster vaccinations with Id-KLH protein (see below and Table 3).
Vaccination of patients in first remission Given the results of the pilot phase, we applied this treatment to a group of 25 patients with advanced-stage follicular lymphoma after an attempt at remission induction with chemotherapy. The characteristics of these patients are shown in Table 2. Chemotherapy was administered to achieve the best clinical response (response plateau with last 2 cycles). Patients were then observed until vaccine production was completed (median, 5 months; range, 3-18 months). After chemotherapy and at the time of vaccination, patients achieved and sustained the following responses: CR in 5 patients, CRu in 11 patients, and PR in 7 patients (designated prevaccine tumor status of NED, MRD, and RD, respectively). Two patients (patients D20 and D27) had progressive disease (PD) at the time of vaccination (both at 9 months after chemotherapy) and completed only 3 vaccinations before they needed chemotherapy and had to be removed from the study. Numbers of DCs administered (median, 16 × 106 over 4 infusions; range, 8-35 × 106 total DCs given, 0.7-12 × 106/infusion) and toxicities observed (less than 20% incidence of transient flulike symptoms) were comparable to those in the pilot phase of the study.Immune responses of patients in first remission Twenty-three patients completed the series of 4 vaccinations and were considered evaluable for immune responses. All 23 patients mounted humoral and cellular proliferative responses to KLH. Overall, 15 of the 23 (65%) patients mounted T-cell or humoral anti-Id responses. In patients D11 to D22, native, unmodified Id protein was used for DC pulsing as in the pilot study. Five of the 11 evaluable patients in this subgroup mounted T-cell anti-Id responses, and anti-Id antibodies of the IgM class, mirroring the low frequency of anti-Id antibodies seen in the pilot phase, developed in one patient. To improve the induction of Id-specific humoral immunity, 13 additional patients (patients D23-D35) were immunized with DCs pulsed with the Id-KLH conjugate. This modification was based on recent studies of Id-pulsed DC vaccination in a murine lymphoma model, demonstrating that the use of Id-KLH conjugate for DC pulsing led to improved induction of anti-Id antibodies and tumor protection over native Id protein.36 Among 12 evaluable patients vaccinated in this manner, 4 mounted cellular anti-Id responses, and 6 developed humoral anti-Id responses. In 4 of these patients, high titers of anti-Id antibodies of the IgG class were detectable. In 2 additional patients, anti-Id antibodies were of the IgM class. The Id specificity of the humoral anti-Id response evoked in 2 representative patients (D29 and D31) after vaccination with Id-KLH-pulsed DCs is shown in Figure 2. These serum antibodies showed a high level of binding to the relevant (autologous) tumor Id protein but a low degree of binding to irrelevant Id proteins from other patients' tumors. To further characterize these humoral anti-Id responses, we next asked whether these sera could recognize tumor Id protein as presented in its native conformation by tumor cells. Tumor cells were incubated with either relevant or control prevaccine or postvaccine serum and were stained for bound anti-Id antibodies. Figure 3 shows a flow cytometric analysis demonstrating the ability of IgG antibodies in postvaccine serum to specifically recognize autologous tumor cells but not control, irrelevant tumor cells from another patient. Postvaccine sera could specifically recognize tumor cells in 4 of 5 patients for whom tumor cells were available for testing.
Evoked antibodies induce signal transduction in tumor B cells We next investigated whether these serum anti-Id antibodies could mediate signal transduction after binding to tumor cells. When B-cell surface immunoglobulin is engaged and cross-linked by antigen, tyrosine kinases are recruited to phosphorylate tyrosine residues in a number of cellular proteins.37 Anti-Id antibodies, because they also specifically cross-link surface immunoglobulin, can transmit a similar signal. Previous studies have established that tumor regression induced by passively administered anti-Id antibodies38 can be correlated with ability of the antibodies to induce tyrosine phosphorylation in tumor cells.35 However, such effects have not been described using anti-Id antibodies from actively vaccinated patients. Therefore, we treated tumor cells with immune sera and assayed for the induction of tyrosine phosphorylation. As shown in the Western blot in Figure 4A, only cells treated with the relevant postvaccine serum displayed the distinct pattern of signal intensities, with more prominent bands at 140 and 75 kd. To verify and further quantitate these effects on a per-cell basis, we used flow cytometric analysis for cellular phosphotyrosine content. Using this method, permeabilized tumor cells stained for total intracellular phosphotyrosine could be counterstained for surface markers to determine the population accounting for the increased signal. As shown in Figure 4B, only treatment of cells with the relevant postimmune serum results in a rightward shift in signal, indicating increased phosphotyrosine content within the CD20+ tumor cell population (left panel), whereas no such shift is seen in the CD3+ tumor-infiltrating T cells (right panel). Mean fluorescence intensities of the phosphotyrosine (4G10) signal induced in the CD20+ cells by prevaccine serum, postvaccine serum, or anti-µm antibodies (positive control) were 48, 103, and 110, respectively. These results indicate that serum anti-Id antibodies induced by active vaccination can initiate signal transduction specifically in tumor B cells. Such effects were observed in 2 of the 3 patients for whom tumor cells were available for testing.
Clinical responses and follow-up of patients in first remission In evaluable patients with less than CR at the time of vaccination (n = 18), sustained regression of residual tumor (in bone marrow, lymph nodes, or pleura) was seen in 4 patients (22%; patients D13, D15, D16, and D29). Patient D29, whose postvaccine sera specifically bound to tumor cells, had regression of lymphomatous pleural disease after vaccination (Figure 5). Pleural effusion shown in the CT images of the chest was refractory to cyclophosphamide, vincristine, and prednisone (CVP) chemotherapy and remained cytologically positive before vaccination. However, 9 months after vaccination with Id-KLH-pulsed DCs, it had almost completely resolved. This patient also had a marked reduction in bone marrow disease after vaccination and has remained without disease progression for more than 21 months. Long-term follow-up of patients vaccinated during first remission shows that 16 of 23 (70%) remain progression free at a median of 43 months after the completion of chemotherapy (range, 23-56 months). There has been a single death (patient D21) associated with a transformation to aggressive diffuse large-cell lymphoma. Six of 7 (86%) patients mounting humoral anti-Id responses have remained progression free compared with 10 of 16 (63%) without antibody responses, though this difference is not statistically significant (P = .40, log-rank statistic). The proportion of patients remaining progression free did not differ between patients mounting T-cell or humoral responses (10 of 15, 67%) versus those without immune responses (6 of 8, 75%).
Administration of booster vaccinations with Id-KLH protein plus chemical adjuvant Patients with persistent or relapsed tumors after DC vaccination were offered booster vaccination with Id-KLH protein without DCs, as performed in our previous vaccine trials.7 Biopsy samples were again taken from tumors of 4 patients before revaccination (patients D5, D6, D10, D12). Each was found to have retained expression of surface immunoglobulin heavy and light chains of the same classes found on the original tumor. Therapy consisted of 5 subcutaneous injections of the same Id protein used for DC vaccination yet was coupled to KLH and was emulsified in a chemical adjuvant.7 Six patients were treated 5 from the pilot study who had not fully responded and 1 who had a relapse after DC vaccination given during first remission (Table 3). The Id-KLH booster vaccinations were given
at different lengths of time after the original DC vaccination series
(range, 8-33 months), and all patients had multiple sites of
progressive, evaluable tumor. Three of the patients had immune responses to the booster vaccine series; 2 were T-cell responses and
one was a humoral response (Table 3). These responses were of the same
type (T cell, humoral) and of similar magnitude to those each patient
previously mounted to the DC vaccine, though the earlier responses had
waned in the interval before boosting. Each of the 3 immune responders
also had a corresponding clinical response; one PR lasting 14 months,
one CR ongoing at 48+ months, and one CRu lasting 16 months. The latter
patient (patient D12), treated 18 months after relapse following DC
vaccination given in first remission, had extensive lymphadenopathy
involving cervical, axillary, iliac, and inguinal lymph nodes,
including a pelvic mass measuring more than 4 × 5 × 6 cm. Bone
marrow was also involved. Within 2 weeks of the first injection of
Id-KLH, all palpable lymphadenopathy began to rapidly regress, and
physical examination results became normal in the next 4 weeks.
Normalization of all nodes was documented by CT at 1 and 4 months after
the completion of vaccination (Figure
6A-B), and the only evidence of tumor was equivocal findings in the bone marrow. At 16 months after vaccination, small recurrent lymph nodes were detected in the right side of the
neck. A repeat series of Id-KLH vaccinations (designated vaccine course
3) was then administered, and tumor regression occurred once again,
with CR documented 7 months later accompanied by an ongoing Id-specific
T-cell proliferative response (Table 3).
Given this patient's repeated tumor regression in response to
vaccination, we looked for evidence of antitumor cytolytic activity in
peripheral blood lymphocyte (PBL) effectors after restimulation with
CD40L-activated tumor cells.39 As shown in Figure
7, restimulated PBLs obtained after
vaccine course 3 exhibited specific cytotoxicity toward autologous
tumor cells. PBL obtained before vaccine course 3 had lower, but
measurable, cytolytic activity. There was no significant lysis of
purified autologous normal B cells (obtained after the attainment of
CR) or of follicular lymphoma cells from a patient of unknown HLA
haplotype (data not shown).
Our earliest studies of Id vaccination for NHL focused on describing the induction of anti-Id immune responses6 and their correlation with favorable clinical outcomes.7 With our more recently developed DC vaccine approach, we directly assessed antitumor effects by vaccination of larger numbers of patients with measurable tumors. In the pilot phase of our DC study, we observed antitumor activity in 4 of 10 patients consisting of 3 objective radiographic responses and 1 molecular complete response. In 3 patients, these responses were complete and durable (44, 57, and 73+ months). Among patients with residual disease in first remission after chemotherapy, 4 of 18 also had objective reductions in measured tumor burden. Two additional patients had tumor shrinkage after Id-KLH booster vaccinations. Thus, 10 of 28 (36%) patients with evaluable tumor had objective antitumor effects after our Id vaccine interventions. A novel finding in this study was the occurrence of tumor regression in patients with resistant or relapsed disease after retreatment with booster Id-KLH protein vaccinations. These observed tumor regressions are instructive of several points. First, revaccination using the same Id protein, reformulated as Id-KLH plus chemical adjuvant, can lead to tumor regression despite the lack of a clinical response to the initial Id-pulsed DC vaccine. Second, even relatively large tumor burdens can undergo regression after Id-KLH vaccination (Figure 6). Whether the prior immunization with Id-pulsed DCs contributed to the observed antitumor responses to Id-KLH is unknown. However, the rapid regression of tumor observed in patient D12, beginning within 2 weeks of Id-KLH initiation, suggests stimulation of a memory response, primed either through the previously administered DC vaccine or through endogenous mechanisms. Taken together, these observations provide unequivocal evidence of the clinical activity of Id vaccination. Induction of anti-Id immune responses was another important endpoint of our study. The overall (humoral or T-cell) immune response rate of 65% compared favorably with the 49% response rate seen in our earlier study of Id-KLH vaccination.7 In keeping with the principal role of DCs in priming T-cell responses, vaccination with Id-pulsed DCs preferentially induced cellular responses over antibody responses (Tables 1-2). Although 62% of patients given native Id-pulsed DCs mounted cellular responses, only 14% produced anti-Id antibodies. However, the ability of DCs to function as adjuvants for the induction of a humoral anti-Id response varied according to the form of Id used for DC pulsing, as previously observed in a murine lymphoma model.36 Vaccination using DCs pulsed with native Id induced detectable anti-Id antibodies, always of the IgM class, in only 3 patients. Use of the Id-KLH conjugate for DC pulsing led to a greater frequency of humoral anti-Id responses (6 of 12 patients; 50%) including high-titer IgG antibodies, once again pointing to the capacity of DCs to prime B-cell responses.18,36 Induction of a humoral anti-Id response is thought to be an important goal of Id vaccination for several reasons. First, passively administered anti-Id murine monoclonal antibodies are able to induce tumor regression in two thirds of patients.38 In addition, in murine lymphoma models of Id vaccination, anti-Id antibodies alone can be sufficient to provide tumor protection.4,13,36,40 Thus, a broad anti-Id immune response, including anti-Id T cells and antibodies, might have greater antitumor effects. Antibodies induced by vaccination with Id-pulsed DCs were highly relevant to tumor cells because they could bind selectively to autologous tumor cells in addition to the isolated Id protein. Furthermore, these antibodies had the capacity to stimulate signal transduction in tumor cells, as indicated by increased tyrosine phosphorylation. Induction of tyrosine phosphorylation by cross-linking surface immunoglobulin on B-lymphoma cells has been shown to be an initial step in the cascade of events leading to apoptosis,37,41,42 suggesting a direct mechanism whereby anti-Id antibodies may exert antitumor effects. Vuist et al35 previously demonstrated that in patients treated with passive anti-Id monoclonal antibodies, achievement of a clinical response was highly correlated with the ability of the antibodies to stimulate tyrosine phosphorylation in tumor cells. Similarly, we have found that among our treated patients, serum anti-Id antibodies capable of binding to tumor cells could induce tyrosine phosphorylation in 2 of 3 patients. Whether this signal transduction response leads to tumor cell apoptosis, correlates with clinical outcome, or can be detected in patients treated with other types of Id vaccines is the subject of ongoing studies. Overall and progression-free survival of our selected group of 35 patients appears favorable in comparison to that historically observed in follicular NHL,43 though a controlled trial would be necessary to ascribe long-term outcomes to the vaccine interventions. Given the clinically significant tumor regressions observed in this study, however, it is possible that the natural history of the disease was altered in some patients. Bendandi et al39 have also described excellent long-term disease-free survival in 18 of 20 patients (median since chemotherapy, 42 months) with follicular lymphoma vaccinated with Id-KLH plus granulocyte macrophage-colony-stimulating factor. Because their study was limited to patients achieving CR after chemotherapy, few comparisons can be made with our current trial in which 20 of 25 patients had less than CR at the time of vaccination. Nevertheless, all but 1 of our 5 patients in CR has remained free of disease progression (Table 2). Bendandi et al39 measured tumor-specific cytokine secretion by patient PBL cocultured with autologous tumor cells in 19 of 20 patients, and they measured antitumor cytotoxic T-cell activity in a subset of these patients. This 95% overall immune response rate is higher than that observed in the current study. The difference may be explained by the use of alternative assay techniques, their use of Id-KLH plus granulocyte macrophage-colony-stimulating factor adjuvant, or by selection of a more favorable first CR patient population. Indeed it does appear from previous work7 and our current study that immune responses after Id vaccination are more frequent in patients in CR at the time of vaccination (4 of 5 immune response-positive in the current study). Although we did not routinely measure antitumor cytotoxic T cells in our patients, in the one patient studied (patient D12) we did find considerable tumor-specific cytolytic activity in PBLs obtained after repeat booster vaccinations with Id-KLH and objective tumor regressions. We believe that our findings have several implications for the design of future lymphoma vaccine trials. Our results in NHL once again confirm tumor antigen-pulsed DC vaccination as a promising platform for cancer immunotherapy.20 This approach is more technically demanding than traditional protein (ie, Id-KLH) vaccination methods, and it remains to be proven superior in formal comparisons. Before embarking on such studies, it will be important to further refine this technique, particularly with regard to the types and numbers of antigen-pulsed DCs administered.19,20 Our use of peripheral blood DCs isolated from leukapheresis product was based on the demonstrated T-cell stimulatory capacity of this DC fraction32,33,44 and its observed clinical activity early in our pilot study.22 Numbers of DCs obtainable using this method, approximately 5 to 10 million per infusion, do not allow for dose-escalation studies. However, newer methodologies for propagating DCs from monocytes45 or CD34+ hematopoietic progenitor cells46 or from the mobilization of peripheral blood DCs using Flt3 ligand28,47 now allow DC dose escalation to more than 108 cells. The use of greater numbers of antigen-pulsed cells, cultured under conditions that promote maturation and activation of DCs,48-50 may provide even greater potency at inducing anti-Id immune and clinical responses. Our observation of significant clinical activity in DC-vaccinated patients after booster vaccination with Id-KLH suggests a potential new strategy for the immunotherapy of follicular NHL, and further studies using Id-KLH vaccines in tandem with Id-pulsed DCs are in progress.51 These observations may also herald a shift in our view of the optimal timing of Id vaccination. Earlier studies of Id vaccination have been principally confined to patients in remission after cytoreductive chemotherapy,6,7,39 based on the view that vaccination might be more effective when the level of tumor is minimal. Thus, little is known about the therapeutic effects of Id-KLH vaccination in patients with progressive lymphoma. Indeed, in our previous study, in vitro anti-Id immune responses were more frequently detectable in patients in CR at the time of vaccination than in those with residual disease,7 suggesting that the presence of tumor might impair the host's ability to mount an effective antitumor response. The finding that substantial tumor burdens may regress after Id-KLH vaccination (Table 3; Figure 6) provides rationale for further study of Id-KLH vaccination in patients with relapsed or even untreated follicular NHL. In the latter case, avoidance of the strongly immunosuppressive effects of chemotherapy before immunization might prove to be an important advantage. Given its validation as a susceptible target, lymphoma idiotype will likely remain a highly relevant tumor antigen for NHL immunotherapy despite the need to produce patient-specific material. In multiple clinical trials, Id vaccine production has been shown to be feasible, and evidence of clinical activity has been obtained.6,22,39 Moreover, novel genetic approaches are now yielding recombinant forms of Id and will continue to make large-scale production of Id vaccines more practical.52-56 These technologies offer new sources of Id for loading into DCs. In the future, as additional and possibly shared NHL-associated tumor antigens are described, these too will be candidates for evaluation using DC-based vaccine strategies.
We thank Hendrik Veelken for critical reading of the manuscript; Dan Denney and Diane Ingolia for help in producing recombinant tumor Id proteins; Yong-Jun Liu at DNAX for the gift of CD40 ligand-transfected L cells; and Patty Ciesla for assistance in preparing the manuscript.
Submitted June 22, 2001; accepted September 14, 2001.
Supported by National Institutes of Health grants HL57443 and CA33399. J.M.T. and T.A.D. are recipients of Clinical Associate Physician awards from the National Institutes of Health (RR-00070-CAP). R.L. is an American Cancer Society Clinical Research Professor.
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked "advertisement" in accordance with 18 U.S.C. section 1734.
Reprints: Ronald Levy, Dept of Medicine, Division of Oncology, Stanford University Medical Center, CCSR Rm 1105, Stanford, CA 94305-5151; e-mail: levy{at}stanford.edu.
1. Horning SJ. Natural history of and therapy for the indolent non-Hodgkin's lymphomas. Semin Oncol. 1993;20:75-88[Medline] [Order article via Infotrieve]. 2. Miller RA, Maloney DG, Warnke R, Levy R. Treatment of B-cell lymphoma with monoclonal anti-idiotype antibody. N Engl J Med. 1982;306:517-522[Medline] [Order article via Infotrieve]. 3. Miller RA, Hart S, Samoszuk M, et al. Shared idiotypes expressed by human B-cell lymphomas. N Engl J Med. 1989;321:851-857[Abstract]. 4. George AJ, Tutt AL, Stevenson FK. Anti-idiotypic mechanisms involved in suppression of a mouse B cell lymphoma, BCL1. J Immunol. 1987;138:628-634[Abstract]. 5. Campbell MJ, Carroll W, Kon S, et al. Idiotype vaccination against murine B cell lymphoma: humoral and cellular responses elicited by tumor-derived immunoglobulin M and its molecular subunits. J Immunol. 1987;139:2825-2833[Abstract]. 6. Kwak LW, Campbell MJ, Czerwinski DK, Hart S, Miller RA, Levy R. Induction of immune responses in patients with B-cell lymphoma against the surface-immunoglobulin idiotype expressed by their tumors. N Engl J Med. 1992;327:1209-1215[Abstract].
7.
Hsu FJ, Caspar CB, Czerwinski D, et al.
Tumor-specific idiotype vaccines in the treatment of patients with B-cell lymphoma: long-term results of a clinical trial.
Blood.
1997;89:3129-3135
8.
Lauritzsen GF, Weiss S, Dembic Z, Bogen B.
Naive idiotype-specific CD4+ T cells and immunosurveillance of B-cell tumors.
Proc Natl Acad Sci U S A.
1994;91:5700-5704
9.
Cao W, Myers-Powell BA, Braciale TJ.
Recognition of an immunoglobulin VH epitope by influenza virus-specific class I major histocompatibility complex-restricted cytolytic T lymphocytes.
J Exp Med.
1994;179:195-202 10. Chakrabarti D, Ghosh SK. Induction of syngeneic cytotoxic T lymphocytes against a B cell tumor, II: characterization of anti-idiotypic CTL lines and clones. Cell Immunol. 1992;144:443-454[CrossRef][Medline] [Order article via Infotrieve]. 11. Abe A, Emi N, Taji H, Kasai M, Kohno A, Saito H. Induction of humoral and cellular anti-idiotypic immunity by intradermal injection of naked DNA encoding a human variable region gene sequence of an immunoglobulin heavy chain in a B cell malignancy. Gene Ther. 1996;3:988-993[Medline] [Order article via Infotrieve].
12.
Osterroth F, Garbe A, Fisch P, Veelken H.
Stimulation of cytotoxic T cells against idiotype immunoglobulin of malignant lymphoma with protein-pulsed or idiotype-transduced dendritic cells.
Blood.
2000;95:1342-1349 13. Campbell MJ, Esserman L, Byars NE, Allison AC, Levy R. Idiotype vaccination against murine B cell lymphoma: humoral and cellular requirements for the full expression of antitumor immunity. J Immunol. 1990;145:1029-1036[Abstract]. 14. George AJ, Folkard SG, Hamblin TJ, Stevenson FK. Idiotypic vaccination as a treatment for a B cell lymphoma. J Immunol. 1988;141:2168-2174[Abstract]. 15. Sugai S, Palmer DW, Talal N, Witz IP. Protective and cellular immune responses to idiotypic determinants on cells from a spontaneous lymphoma of NZB-NZW F1 mice. J Exp Med. 1974;140:1547-1558[Abstract].
16.
Kwak LW, Young HA, Pennington RW, Weeks SD.
Vaccination with syngeneic, lymphoma-derived immunoglobulin idiotype combined with granulocyte/macrophage colony-stimulating factor primes mice for a protective T-cell response.
Proc Natl Acad Sci U S A.
1996;93:10972-10977 17. Campbell MJ, Esserman L, Levy R. Immunotherapy of established murine B cell lymphoma: combination of idiotype immunization and cyclophosphamide. J Immunol. 1988;141:3227-3233[Abstract]. 18. Banchereau J, Steinman RM. Dendritic cells and the control of immunity. Nature. 1998;392:245-252[CrossRef][Medline] [Order article via Infotrieve]. 19. Banchereau J, Briere F, Caux C, et al. Immunobiology of dendritic cells. Annu Rev Immunol. 2000;18:767-811[CrossRef][Medline] [Order article via Infotrieve]. 20. Timmerman JM, Levy R. Dendritic cell vaccines for cancer immunotherapy. Annu Rev Med. 1999;50:507-529[CrossRef][Medline] [Order article via Infotrieve]. 21. Flamand V, Sornasse T, Thielemans K, Demanet C, Bakkus M, et al. Murine dendritic cells pulsed in vitro with tumor antigen induce tumor resistance in vivo. Eur J Immunol. 1994;24:605-610[Medline] [Order article via Infotrieve]. 22. Hsu FJ, Benike C, Fagnoni F, et al. Vaccination of patients with B-cell lymphoma using autologous antigen-pulsed dendritic cells. Nat Med. 1996;2:52-58[CrossRef][Medline] [Order article via Infotrieve]. 23. Nestle FO, Alijagic S, Gilliet M, et al. Vaccination of melanoma patients with peptide- or tumor lysate-pulsed dendritic cells [see comments]. Nat Med. 1998;4:328-332[CrossRef][Medline] [Order article via Infotrieve].
24.
Thurner B, Haendle I, Roder C, et al.
Vaccination with mage-3A1 peptide-pulsed mature, monocyte-derived dendritic cells expands specific cytotoxic T cells and induces regression of some metastases in advanced stage IV melanoma.
J Exp Med.
1999;190:1669-1678 25. Murphy GP, Tjoa BA, Simmons SJ, et al. Phase II prostate cancer vaccine trial: report of a study involving 37 patients with disease recurrence following primary treatment. Prostate. 1999;39:54-59[CrossRef][Medline] [Order article via Infotrieve]. 26. Murphy GP, Tjoa BA, Simmons SJ, et al. Infusion of dendritic cells pulsed with HLA-A2-specific prostate-specific membrane antigen peptides: a phase II prostate cancer vaccine trial involving patients with hormone-refractory metastatic disease. Prostate. 1999;38:73-78[CrossRef][Medline] [Order article via Infotrieve]. 27. Kugler A, Stuhler G, Walden P, et al. Regression of human metastatic renal cell carcinoma after vaccination with tumor cell-dendritic cell hybrids [see comments]. Nat Med. 2000;6:332-336[CrossRef][Medline] [Order article via Infotrieve].
28.
Fong L, Hao Y, Rivas A, et al.
Altered peptide ligand vaccination with Flt3 ligand expanded dendritic cells for tumor immunotherapy.
Proc Natl Acad Sci U S A.
2001;98:8809-8814
29.
Cheson BD, Horning SJ, Coiffier B, et al.
Report of an international workshop to standardize response criteria for non-Hodgkin's lymphomas: NCI Sponsored International Working Group [published erratum appears in J Clin Oncol 2000;18:2351].
J Clin Oncol.
1999;17:1244 30. Campbell MJ, Zelenetz AD, Levy S, Levy R. Use of family specific leader region primers for PCR amplification of the human heavy chain variable region gene repertoire. Mol Immunol. 1992;29:193-203[CrossRef][Medline] [Order article via Infotrieve]. 31. Okada CY, Wong CP, Denney DW, Levy R. TCR vaccines for active immunotherapy of T cell malignancies. J Immunol. 1997;159:5516-5527[Abstract]. 32. Mehta-Damani A, Markowicz S, Engleman EG. Generation of antigen-specific CD4+ T cell lines from naive precursors. Eur J Immunol. 1995;25:1206-1211[Medline] [Order article via Infotrieve]. 33. Mehta-Damani A, Markowicz S, Engleman EG. Generation of antigen-specific CD8+ CTLs from naive precursors. J Immunol. 1994;153:996-1003[Abstract].
34.
Schultze JL, Seamon MJ, Michalak S, Gribben JG, Nadler LM.
Autologous tumor infiltrating T cells cytotoxic for follicular lymphoma cells can be expanded in vitro.
Blood.
1997;89:3806-3816
35.
Vuist WM, Levy R, Maloney DG.
Lymphoma regression induced by monoclonal anti-idiotypic antibodies correlates with their ability to induce Ig signal transduction and is not prevented by tumor expression of high levels of bcl-2 protein.
Blood.
1994;83:899-906
36.
Timmerman JM, Levy R.
Linkage of foreign carrier protein to a self-tumor antigen enhances the immunogenicity of a pulsed dendritic cell vaccine.
J Immunol.
2000;164:4797-4803 37. Sefton BM, Campbell MA. The role of tyrosine protein phosphorylation in lymphocyte activation. Annu Rev Cell Biol. 1991;7:257-274[CrossRef].
38.
Davis TA, Maloney DG, Czerwinski DK, Liles TM, Levy R.
Anti-idiotype antibodies can induce long-term complete remissions in non-Hodgkin's lymphoma without eradicating the malignant clone.
Blood.
1998;92:1184-1190 39. Bendandi M, Gocke CD, Kobrin CB, et al. Complete molecular remissions induced by patient-specific vaccination plus granulocyte-monocyte colony-stimulating factor against lymphoma [see comments]. Nat Med. 1999;5:1171-1177[CrossRef][Medline] [Order article via Infotrieve].
40.
Syrengelas AD, Levy R.
DNA vaccination against the idiotype of a murine B cell lymphoma: mechanism of tumor protection.
J Immunol.
1999;162:4790-4795 41. Benhamou LE, Cazenave PA, Sarthou P. Anti-immunoglobulins induce death by apoptosis in WEHI-231 B lymphoma cells. Eur J Immunol. 1990;20:1405-1407[Medline] [Order article via Infotrieve]. 42. Hasbold J, Klaus GG. Anti-immunoglobulin antibodies induce apoptosis in immature B cell lymphomas. Eur J Immunol. 1990;20:1685-1690[Medline] [Order article via Infotrieve]. 43. Armitage JO, Weisenburger DD. New approach to classifying non-Hodgkin's lymphomas: clinical features of the major histologic subtypes: Non-Hodgkin's Lymphoma Classification Project. J Clin Oncol. 1998;16:2780-2795[Abstract].
44.
Freudenthal PS, Steinman RM.
The distinct surface of human blood dendritic cells, as observed after an improved isolation method.
Proc Natl Acad Sci U S A.
1990;87:7698-7702 45. Thurner B, Roder C, Dieckmann D, et al. Generation of large numbers of fully mature and stable dendritic cells from leukapheresis products for clinical application [published erratum appears in J Immunol Methods 199922;224(1-2):211]. J Immunol Methods. 1999;223:1-15[CrossRef][Medline] [Order article via Infotrieve]. 46. Banchereau J, Palucka K, Dhodapkhar M, et al. Immune and clinical responses after vaccination of patients with metastatic melanoma with CD34+ HPC-derived dendritic cells [abstract]. Keystone Symposia-Dendritic Cells: Interfaces with Immunobiology and Medicine. Taos, NM:; 2001:71.
47.
Maraskovsky E, Daro E, Roux E, et al.
In vivo generation of human dendritic cell subsets by Flt3 ligand.
Blood.
2000;96:878-884
48.
Reddy A, Sapp M, Feldman M, Subklewe M, Bhardwaj N.
A monocyte conditioned medium is more effective than defined cytokines in mediating the terminal maturation of human dendritic cells.
Blood.
1997;90:3640-3646
49.
Morse MA, Lyerly HK, Gilboa E, Thomas E, Nair SK.
Optimization of the sequence of antigen loading and CD40-ligand-induced maturation of dendritic cells.
Cancer Res.
1998;58:2965-2968
50.
Hartmann G, Weiner GJ, Krieg AM.
CpG DNA: a potent signal for growth, activation, and maturation of human dendritic cells.
Proc Natl Acad Sci U S A.
1999;96:9305-9310 51. Hsu F, Komarovskaya M, Song B, Doyon AG. Active immunotherapy of patients with lymphoma with sequential dendritic cell and protein/adjuvant idiotype vaccines [abstract]. Blood. 1999;94:386. 52. Timmerman JM, Czerwinski D, Taid B, et al. A phase I/II trial to evaluate the immunogenicity of recombinant idiotype protein vaccines for the treatment of non-Hodgkin's lymphoma (NHL) [abstract]. Blood. 2000;96:578.
53.
Timmerman JM, Caspar CB, Lambert SL, Syrengelas AD, Levy R.
Idiotype-encoding recombinant adenoviruses provide protective immunity against murine B-cell lymphomas.
Blood.
2001;97:1370-1377
54.
McCormick AA, Kumagai MH, Hanley K, et al.
Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants.
Proc Natl Acad Sci U S A.
1999;96:703-708 55. King CA, Spellerberg MB, Zhu D, et al. DNA vaccines with single-chain Fv fused to fragment C of tetanus toxin induce protective immunity against lymphoma and myeloma. Nat Med. 1998;4:1281-1286[CrossRef][Medline] [Order article via Infotrieve]. 56. Osterroth F, Alkan O, Mackensen A, et al. Rapid expression cloning of human immunoglobulin Fab fragments for the analysis of antigen specificity of B cell lymphomas and anti-idiotype lymphoma vaccination. J Immunol Methods. 1999;229:141-153[CrossRef][Medline] [Order article via Infotrieve].
© 2002 by The American Society of Hematology.
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||||
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||||
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||||
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||||
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||||
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||||
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||||
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||||
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||||
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||||
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||||
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||||
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||||
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||||
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||||
![]() |
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||||
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||||
![]() |
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||||
![]() |
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||||
![]() |
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||||
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||||
![]() |
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Y. Sakai, B. J. Morrison, J. D. Burke, J.-M. Park, M. Terabe, J. E. Janik, G. Forni, J. A. Berzofsky, and J. C. Morris Vaccination by Genetically Modified Dendritic Cells Expressing a Truncated neu Oncogene Prevents Development of Breast Cancer in Transgenic Mice Cancer Res., November 1, 2004; 64(21): 8022 - 8028. [Abstract] [Full Text] [PDF] |
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D. W. O'Neill, S. Adams, and N. Bhardwaj Manipulating dendritic cell biology for the active immunotherapy of cancer Blood, October 15, 2004; 104(8): 2235 - 2246. [Abstract] [Full Text] [PDF] |
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Q. Lou, R. J. Kelleher Jr, A. Sette, J. Loyall, S. Southwood, R. B. Bankert, and S. H. Bernstein Germ line tumor-associated immunoglobulin VH region peptides provoke a tumor-specific immune response without altering the response potential of normal B cells Blood, August 1, 2004; 104(3): 752 - 759. [Abstract] [Full Text] [PDF] |
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D. Avigan, B. Vasir, J. Gong, V. Borges, Z. Wu, L. Uhl, M. Atkins, J. Mier, D. McDermott, T. Smith, et al. Fusion Cell Vaccination of Patients with Metastatic Breast and Renal Cancer Induces Immunological and Clinical Responses Clin. Cancer Res., July 15, 2004; 10(14): 4699 - 4708. [Abstract] [Full Text] [PDF] |
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J. N. Blattman and P. D. Greenberg Cancer Immunotherapy: A Treatment for the Masses Science, July 9, 2004; 305(5681): 200 - 205. [Abstract] [Full Text] [PDF] |
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C. L. van Broekhoven, C. R. Parish, C. Demangel, W. J. Britton, and J. G. Altin Targeting Dendritic Cells with Antigen-Containing Liposomes: A Highly Effective Procedure for Induction of Antitumor Immunity and for Tumor Immunotherapy Cancer Res., June 15, 2004; 64(12): 4357 - 4365. [Abstract] [Full Text] [PDF] |
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S. Paczesny, J. Banchereau, K. M. Wittkowski, G. Saracino, J. Fay, and A. K. Palucka Expansion of Melanoma-specific Cytolytic CD8+ T Cell Precursors in Patients with Metastatic Melanoma Vaccinated with CD34+ Progenitor-derived Dendritic Cells J. Exp. Med., June 7, 2004; 199(11): 1503 - 1511. [Abstract] [Full Text] [PDF] |
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M. Bendandi, S. A. Pileri, and P. L. Zinzani Challenging paradigms in lymphoma treatment Ann. Onc., May 1, 2004; 15(5): 703 - 711. [Full Text] [PDF] |
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J. G. G. Gilles, S. C. Grailly, M. De Maeyer, M. G. Jacquemin, L. P. VanderElst, and J.-M. R. Saint-Remy In vivo neutralization of a C2 domain-specific human anti-Factor VIII inhibitor by an anti-idiotypic antibody Blood, April 1, 2004; 103(7): 2617 - 2623. [Abstract] [Full Text] [PDF] |
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M. Y. Mapara and M. Sykes Tolerance and Cancer: Mechanisms of Tumor Evasion and Strategies for Breaking Tolerance J. Clin. Oncol., March 15, 2004; 22(6): 1136 - 1151. [Abstract] [Full Text] [PDF] |
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M. A. Caligiuri, A. Velardi, D. A. Scheinberg, and I. M. Borrello Immunotherapeutic Approaches for Hematologic Malignancies Hematology, January 1, 2004; 2004(1): 337 - 353. [Abstract] [Full Text] [PDF] |
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T. Maier, A. Tun-Kyi, A. Tassis, K.-P. Jungius, G. Burg, R. Dummer, and F. O. Nestle Vaccination of patients with cutaneous T-cell lymphoma using intranodal injection of autologous tumor-lysate-pulsed dendritic cells Blood, October 1, 2003; 102(7): 2338 - 2344. [Abstract] [Full Text] [PDF] |
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D. Winter, E. Fiebiger, P. Meraner, H. Auer, C. Brna, R. Strohal, F. Trautinger, R. Knobler, G. F. Fischer, G. Stingl, et al. Definition of TCR Epitopes for CTL-Mediated Attack of Cutaneous T Cell Lymphoma J. Immunol., September 1, 2003; 171(5): 2714 - 2724. [Abstract] [Full Text] [PDF] |
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K. U. Lundin, P. O. Hofgaard, H. Omholt, L. A. Munthe, A. Corthay, and B. Bogen Therapeutic effect of idiotype-specific CD4+ T cells against B-cell lymphoma in the absence of anti-idiotypic antibodies Blood, July 15, 2003; 102(2): 605 - 612. [Abstract] [Full Text] [PDF] |
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H.-W. Chen, Y.-P. Lee, Y.-F. Chung, Y.-C. Shih, J.-P. Tsai, M.-H. Tao, and C.-C. Ting Inducing long-term survival with lasting anti-tumor immunity in treating B cell lymphoma by a combined dendritic cell-based and hydrodynamic plasmid-encoding IL-12 gene therapy Int. Immunol., March 1, 2003; 15(3): 427 - 435. [Abstract] [Full Text] [PDF] |
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I. D. Davis, M. Jefford, P. Parente, and J. Cebon Rational approaches to human cancer immunotherapy J. Leukoc. Biol., January 1, 2003; 73(1): 3 - 29. [Abstract] [Full Text] [PDF] |
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E. Gatza and C. Y. Okada Tumor Cell Lysate-Pulsed Dendritic Cells Are More Effective Than TCR Id Protein Vaccines for Active Immunotherapy of T Cell Lymphoma J. Immunol., November 1, 2002; 169(9): 5227 - 5235. [Abstract] [Full Text] [PDF] |
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J. M. Timmerman, G. Singh, G. Hermanson, P. Hobart, D. K. Czerwinski, B. Taidi, R. Rajapaksa, C. B. Caspar, A. van Beckhoven, and R. Levy Immunogenicity of a Plasmid DNA Vaccine Encoding Chimeric Idiotype in Patients with B-Cell Lymphoma Cancer Res., October 15, 2002; 62(20): 5845 - 5852. [Abstract] [Full Text] [PDF] |
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H. Ben-Bassat, Z. Hartzstark, R. Levitzki, B. Y. Klein, Z. Shlomai, A. Gazit, and A. Levitzki Tyrosine Kinase Inhibitors Suppress the Growth of Non-Hodgkin B Lymphomas J. Pharmacol. Exp. Ther., October 1, 2002; 303(1): 163 - 171. [Abstract] [Full Text] [PDF] |
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B. Schuler-Thurner, E. S. Schultz, T. G. Berger, G. Weinlich, S. Ebner, P. Woerl, A. Bender, B. Feuerstein, P. O. Fritsch, N. Romani, et al. Rapid Induction of Tumor-specific Type 1 T Helper Cells in Metastatic Melanoma Patients by Vaccination with Mature, Cryopreserved, Peptide-loaded Monocyte-derived Dendritic Cells J. Exp. Med., May 20, 2002; 195(10): 1279 - 1288. [Abstract] [Full Text] [PDF] |
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J. M. Vose, B. C.-H. Chiu, B. D. Cheson, J. Dancey, and J. Wright Update on Epidemiology and Therapeutics for Non-Hodgkin's Lymphoma Hematology, January 1, 2002; 2002(1): 241 - 262. [Abstract] [Full Text] |
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