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Blood, Vol. 109, Issue 9, 3856-3864, May 1, 2007
Stimulation of P2 receptors causes release of IL-1ß–loaded microvesicles from human dendritic cells
Blood Pizzirani et al.
109: 3856
Supplemental materials for: Pizzirani et al, Vol 109, Issue 9, 3856-3864
Files in this Data Supplement:
- Video S1. Extracellular BzATP triggers microvesicle shedding from human DCs (MOV, 3 MB)
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Immature monocyte-derived DCs seeded on a glass coverslip at a concentration of 105/mL were stimulated with 30 µM BzATP at 37°C in standard saline solution. Microvesicle shedding was analyzed by mounting coverslips in a thermostatted Leyden chamber (set at 38°C; model TC-202A; Medical Systems, Greenvale, NY), placed on the stage of an inverted Nikon Eclipse TE300 microscope (Nikon, Tokyo, Japan). The images were captured at 5-second intervals with a back-illuminated CCD camera (Princeton Instruments, Trenton, NJ) using the Metamorph software (Universal Imaging, West Chester, PA) as described in “Materials and methods,” under “Microscopic analysis.”
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