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Blood, Vol. 108, Issue 6, 1821-1829, September 15, 2006
Transforming growth factor- 1 regulates macrophage migration via RhoA
Blood Kim et al.
108: 1821
Supplemental materials for: Kim et al
Files in this Data Supplement:
- Figure S1. Cytoskeletal rearrangement and ERK are involved in TGF-β1-induced cell migration (JPG, 53 KB)
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Cells were incubated in the upper reservoir with or without a variety of MAPK inhibitors (30 µM PD98059, 30 µM SB203580, and 40 µM SP600125) for 30 min. The lower chamber was then filled with 5 ng/ml TGF- 1, and migration measured as described in the legend to Fig .1. The values are means ± SE (n=3), and each value was compared to the corresponding control (**, P<0.01) (A). Cells were incubated with or without 5 ng/ml TGF-1 for the indicated times at 37°C. Phosphorylation of p38 MAPK, ERK, and JNK and RhoA was analyzed by Western blotting using anti-phospho-p38 MAPK, -ERK, -JNK, and anti-RhoA antibodies (B).
- Figure S2. LPS induces MIP-1α, and this is inhibited by TGF-β1 (JPG, 36.2 KB)
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Cells were incubated with or without 1 µg/ml LPS, 5 ng/ml TGF- 1, 10 µg/ml Tat-C3, or 30 µM H89 for 6 h, and then incubated with 1 µg/ml LPS for 6 h. LPS increased MIP-1 transcription for 6 h, and TGF-1, and Tat-C3 blocked this increased transcription, and this inhibition was reversed by H89.
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