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Blood, Vol. 109, Issue 3, 1211-1219, February 1, 2007
Bcr-Abl signaling through the PI-3/S6 kinase pathway inhibits nuclear translocation of the transcription factor Bach2, which represses the antiapoptotic factor heme oxygenase-1
Blood Yoshida et al.
109: 1211
Supplemental materials for: Yoshida et al, Vol 109, Issue 3, 1211-1219
Files in this Data Supplement:
- Figure S1. 3T3 cells were transiently transfected with pEGFPBach2 plasmids, and the subcellular localization of GFP-Bach2 was observed through a fluorescence microscope (PDF, 31 KB) -
Representative images of cells expressing GFP-Bach2 (green) categorized as being localized mainly in the cytoplasm (C > N), equally in the cytoplasm and nucleus (C = N), and mainly in the nucleus (C < N). GFP (left panels), DAPI-stained DNA (blue, middle panels), and merged (right panels) images are shown. The coverslips were mounted in DAPI-containing Vectashield (Vector Labs, Burlingame, CA). Slides were examined under a 40×/1.0 NA oil objective through an Olympus BX-41 fluorescence microscope (Tokyo, Japan). Images were captured using an Orca AG digital camera (Hamamatsu Photonics UK Ltd, Hertfordshire, United Kingdom) and the Smartcapture X Software (Digital Scientific, Cambridge, United Kingdom), and processed with Adobe PhotoShop CS (Adobe Systems Inc., San Jose, CA).
- Figure S2. BV173 cells were incubated with the indicated inhibitors, and immunoblots were probed with the 2G11 anti-Bach2 monoclonal antibody (PDF, 63.1 KB)
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