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Blood, Vol. 108, Issue 7, 2182-2189, October 1, 2006 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Two novel activating mutations in the Wiskott-Aldrich syndrome protein result in congenital neutropenia Blood Ancliff et al. 108: 2182 Supplemental materials for: Ancliff et al, Vol 108, Issue 7, 2182-2189 Files in this Data Supplement: Document S1. Supplemental methods (PDF, 129 KB) - Details include quantification of WASp expression and live-cell analysis. Video 1. IRM film of human peripheral-blood–derived macrophages from a healthy individual plated on poly-L-lysine–coated glass viewing chambers (MOV, 4.03 MB) - Micrographs were taken 10 seconds apart and displayed at 10 frames per second. Normal macrophages formed dynamic podosomes that assembled and disassembled continuously behind the leading edge. Video 2: IRM film of human peripheral-blood–derived macrophages from a WAS patient deficient in the expression of WASP plated on poly-L-lysine–coated glass viewing chambers (MOV, 4.15 MB) - Micrographs were taken 10 seconds apart and displayed at 10 frames per second. These macrophages failed to develop a patent leading edge, and podosomes were replaced by adhesions that were highly stable compared with those in normal cells. Video 3: IRM film of human peripheral-blood–derived macrophages from patient P1 with the mutation WASp-294Thr plated on poly-L-lysine–coated glass viewing chambers (MOV, 4.56 MB) - Micrographs were taken 10 seconds apart and displayed at 10 frames per second. Podosomes assembled by these cells are more highly dynamic compared with podosomes in normal macrophages. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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