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Blood, Vol. 110, Issue 7, 2600-2609, October 1, 2007
NPM/ALK binds and phosphorylates the RNA/DNA-binding protein PSF in anaplastic large-cell lymphoma
Blood Galietta et al.
110: 2600
Supplemental materials for Galietta et al, Vol 110, Issue 7, 2600-2609
Files in this Data Supplement:
- Document 1. Supplemental materials and methods (PDF, 11.9 KB)
- Figure S1. RNA binding activity of PSF (JPG, 20.1 KB)
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32P end-labelled oligonucleotides corresponding to a consensus pre-mRNA intronic 3′ polypyrimidine tract or to a scrambled sequence (lanes 5-8) of equivalent length and base composition were incubated with oligo alone (lane 1,5), with purified recombinant PSF alone (lane 2,6), purified recombinant ALK kinase domain alone (lane 4,8), or with PSF that had been preincubated with ALK kinase domain under conditions to allow tyrosine phosphorylation (lane 3,7). RNA-protein complexes were resolved by native-PAGE and visualized by autoradiography.
- Figure S2. Relative expression of GAGE6 mRNA, as assessed by real time PCR in 293T cells transiently transfected with empty vector, PSF alone, PSF Y293F, NPM/ALK, NPM/ALK cotransfected with PSF and NPM/ALK cotransfected with PSF Y293F (JPG, 48.4 KB)
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The bar heights represent the means and the error bars the standard error of three separate experiments.
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