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Blood, Vol. 109, Issue 2, 533-542, January 15, 2007
The extracellular nucleotide UTP is a potent inducer of hematopoietic stem cell migration
Blood Rossi et al.
109: 533
Supplemental materials for: Rossi et al, Vol 109, Issue 2, 533-542
Files in this Data Supplement:
- Table S1. Sample name, GEO accession number, and release date (PDF, 11.9 KB)
- Table S2. Increased UTP versus CTR (PDF, 63.5 KB)
- Figure S1. Chemotaxis and in vitro migration of CD34+ HSCs in response to dinucleotide analogs (PDF, 86.8 KB) -
The chemotactic effect of ATP/UTP stable analogs on CD34+ HSCs was assessed in transwell migration assays, as described in “Materials and methods,” under “Chemotaxis assays.” As expected, UTP significantly enhanced CXCL12-induced chemotaxis, and its activity was similar to that of INS415 and INS45973 analogs (P < .05 compared with CXCL12 alone; A). Moreover, UTP alone significantly improved HSC migration rate when combined with CXCL12 in the lower chamber of transwell assays as well as INS415 analog (P < .05; B). These results were confirmed by clonogenic assays (C-D). Results are expressed as mean ± SEM and were obtained from 3 independent experiments. *P values less than .05.
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