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Blood, Vol. 109, Issue 9, 3649-3657, May 1, 2007 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients Blood Lécureuil et al. 109: 3649 Supplemental materials for: Lécureuil et al, Vol 109, Issue 9, 3649-3657 Files in this Data Supplement: Figure S1. In vitro functional assay of CD4+ and CD8+ T cells expressing CCR6 (PDF, 67.5 KB) - Representative staining for cytokine production of CD4 and CD8 T cells following TCR stimulation (A-D). Freshly isolated peripheral blood lymphocytes (PBLs) of uninfected individuals were stimulated with SEB (E) for 12 hours in the presence of brefeldin A for cytokine production assay. CD4+ T cells and CD8+ T cells were then analyzed by flow cytometric analysis as a function of CCR6 expression for their production of IFN-, IL-2, and TNF-α in the 2 memory subsets defined as TCM (CD45RA−/CCR7+) and TEM (CD45RA−/CCR7−). Results are shown as the percentage of cytokine secreting CD4+ or CD8+ T cells expressing or not CCR6 and represent 5 independent experiments. *P < .05; **P < .01. Figure S2. Analysis of phenotypical changes in subpopulations expressing or not expressing CCR6 and/or CCR7 following polyclonal stimulation (PDF, 39.2 KB) - CD45RA− PBMCs were sorted according to their expression of CCR6 and/or CCR7. Cells were then stimulated overnight with or without polyclonal stimuli. Figure S3. Representative staining of CCR6 expression on CD4 and CD8 T cells in uninfected individuals and patients with HIV (PDF, 101 KB) This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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