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Blood, Vol. 109, Issue 9, 4020-4022, May 1, 2007
Flt3 is dispensable to the Hoxa9/Meis1 leukemogenic cooperation
Blood Morgado et al.
109: 4020
Supplemental materials for: Morgado et al, Vol 109, Issue 9, 4020-4022
Files in this Data Supplement:
- Table S1. Characteristics of Flt3+/+ Hoxa9/Meis1 leukemias (PDF, 14.5 KB)
- Figure S1. The protein c-Myb is highly expressed in Hoxa9/Meis1-transduced cells independently of Flt3 (PDF, 14.5 KB) -
Western-blot analysis of cells pooled from primary methylcellulose colonies resulting from the seeding of Flt3+/+ or Flt3–/– cells infected with MSCVneo, Hoxa9, or the bicistronic Hoxa9/Meis1 vectors. The blots were hybridized with specific antibodies directed against c-Myb (mouse monoclonal antibody clone 1-1; Upstate, Lake Placid, NY) or actin isoforms (rabbit polyclonal; Sigma, Saint Louis, MO).
- Figure S2. The morphology of Hoxa9/Meis1 myeloblasts is not influenced by Flt3 expression (PDF, 33.9 KB) -
Wright Giemsa stain of cytospin preparations of BM from Hoxa9/Meis1 leukemic mice (identities of the Hoxa9/Meis1 A9M animals are indicated in the top left corner to allow comparison with Table S1 and Figure 1D). Coverslips were mounted with Coverquick (Labonord, Templemars France) and cells were observed with a Leica DMRB microscope, using a 100×/1.30 NA oil objective (Leica, Wetzlar, Germany). Pictures were obtained using a Canon Powershot S50 camera (Canon, Tokyo, Japan) and the acquisition software RemoteCapture version 2.7.0.10 (Canon).
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