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Blood, Vol. 109, Issue 3, 1147-1155, February 1, 2007
PI3K regulates pleckstrin-2 in T-cell cytoskeletal reorganization
Blood Bach et al.
109: 1147
Supplemental materials for: Bach et al, Vol 109, Issue 3, 1147-1155
Files in this Data Supplement:
- Video 1. Effect of Latrunculin-A on pleckstrin-2-induced cellular spreading (AVI, 5.32 MB)
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Jurkat cells expressing GFP fused to the amino-terminus of wild type pleckstrin-2, were plated on OKT3. Real time confocal imaging of live cells was performed. Shown is a cell expressing pleckstrin-2, before and after inhibition of the actin cytoskeleton with Latrunculin-A for 60 seconds. Images were captured at ∼10 second intervals and are displayed at 5 frames per second. The duration of observation was ∼14.5 minutes, and Latrunculin-A was added after imagining the cells for ∼5 minutes.
- Video 2. Effect of Wortmannin on the membrane association of pleckstrin-2 in Jurkat cells (AVI, 362 KB)
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Jurkat cells expressing GFP fused to the amino-terminus of wild type pleckstrin-2, were plated on fibronectin. When indicated, Wortmannin was added to the chamber slide to a final concentration of 100 nM. Images were captured at ∼15 second intervals, and are displayed at 5 frames per second. The duration of observation was ∼13 minutes, and Wortmannin was added after imaging the cells for ∼4 minutes.
- Video 3. The membrane association of pleckstrin-2 in 2B4 cells is disrupted by LY294002 (AVI, 156 KB)
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2B4 T-cells expressing GFP fused to the amino-terminus of wild type pleckstrin-2, were plated on fibronectin. Images were captured at ∼20 second intervals, and are displayed at 5 frames per second. The duration of observation was ∼8 minutes, and 50 µM LY294002 was added after imaging the cells for ∼4 minutes.
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