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Blood, Vol. 109, Issue 1, 228-234, January 1, 2007
Nitric oxide plays a critical role in suppression of T-cell proliferation by mesenchymal stem cells
Blood Sato et al.
109: 228
Supplemental materials for: Sato et al, Vol 109, Issue 1, 228-234
Files in this Data Supplement:
- Figure S1. Characterization of mouse MSCs (PDF, 202 KB) -
(A) Flow cytometric analysis of cell-surface markers of MSCs. (B) Differentiation of MSCs into adipocytes and osteoblasts. For adipocytic differentiation, cells were treated with 5 µg/mL insulin (Sigma, St Louis, MO) and 10–8 M dexamethasone (Sigma); and for osteoblastic differentiation, cells were treated with 10–8 M dexamethasone, 5 µg/mL ascorbic acid (Sigma), and 10 mM  -glycerophosphate (Sigma).1 Adipocytic differentiation was assessed by staining lipid deposits in cells with Oil red O (Chemicon, Temecula, CA), and osteoblastic differentiation was assessed by staining the cells for alkaline phosphatase activity using an alkaline phosphatase kit (Sigma).2
- Figure S2. Dose-dependent suppression of NO production by L-NAME, a specific NOS inhibitor (PDF, 10.4 KB) -
Splenocytes (1 × 106) were activated with Con A in the presence of 1 × 105 MSCs for 48 hours in the medium containing different concentrations of L-NAME as indicated. NO concentrations were determined by Griess assay as described in Figure 3.
REFERENCES
1. Meirelles Lda S, Nardi NB. Murine marrow-derived mesenchymal stem cell: isolation, in vitro expansion, and characterization. Br J Haematol. 2003;123:702-711.
2. Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284:143-147.
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