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Blood, Vol. 108, Issue 12, 3792-3800, December 1, 2006 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Stimulated plasmacytoid dendritic cells impair human T-cell development Blood Schmidlin et al. 108: 3792 Supplemental materials for: Schmidlin et al, Vol 108, Issue 12, 3792-3800 Files in this Data Supplement: Table S1. Exogenously added IFN-α interferes with the development of thymic CD34+CD1a– precursors into T cells (XLS, 17.5 KB) - Percentages of CD1a+ (analyzed between days 6 and 8), CD4+CD8+, and CD3+ cells (analyzed between days 14 and 18) developing from thymic precursors on OP9-DL1 cells with IL-7 and increasing concentrations of exogenously added IFN- were determined using flow cytometric analysis. Statistical analysis was performed using a paired 2-tailed Student t test. P values indicated were calculated for the percentage of positive cells at the respective concentration versus percentage of positive cells at 0 U/mL (P value 1), 10 U/mL (P value 2), or 1000 U/mL (P value 3) of IFN-. Figure S1. Exogenously added IFN-α hampers the development of human thymic CD34+ precursors in a hybrid human-mouse fetal thymic organ culture (JPEG, 104 KB) - Sorted human thymic CD34+ progenitor cells were cocultured with fetal thymic lobes dissected from embryos of RAG 1–deficient mice in the absence or presence of IFN-. Flow cytometric analysis was performed on days 7 and 10 after staining with antibodies against CD4, CD8, and CD1a. Numbers indicate the percentages of cells within the electronic gate. Shaded curves represent isotype controls; open curves, specific stainings. MFI indicates mean fluorescence intensity. Results are representative of 2 independent experiments. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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