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Blood, Vol. 108, Issue 8, 2755-2763, October 15, 2006
Gene-expression profiling of Waldenström macroglobulinemia reveals a phenotype more similar to chronic lymphocytic leukemia than multiple myeloma
Blood Chng et al.
108: 2755
Supplemental materials for: Chng et al
Files in this Data Supplement:
- Table S1. Gene lists (XLS, 326 KB)
- Figure S1. Unsupervised clustering of WM samples (JPG, 313 KB)
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Using all genes, the unsupervised clustering of WM samples did not identify any prominent clusters. Importantly, 6q deleted and non-deleted cases do not form distinct clusters. In general, WM samples have relatively homogenous gene expression profiles. 6 samples over-expressed proliferation genes corresponding to samples with pre-B cell contamination. 4 samples have reduced expression of a cluster of genes. These genes are down-regulated in MGUS and normal PCs samples suggesting contamination with normal PCs. The colored bar at the bottom of the heatmap represents chromosome 6q deletion status: yellow = deleted, red = non-deleted, blue = unknown. The scale of the gene expression data is similar to figure 1a.
- Figure S2. Expression of genes encoding CD markers in normal B cells, CLL, WM, MM and normal PCs (JPG, 93.7 KB)
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(A) The pattern of expression in WM was more similar to normal B cells and CLL than MM and normal PCs. (B) The appropriate expression of markers known to be differentially expressed in CLL, WM and MM (e.g. CD5, CD19, CD20, CD23, CD56, CD138) provide internal validation to our analysis. There were no obvious new markers specific to WM, CLL or MM except for CD200 that was highly expressed in CLL but not the other conditions. CD1c expression was different between WM and CLL but expression is seen in normal B cells. High expression of CD163 and MME (CD10) in WM is probably due to contaminating monocytes and pre-B cells. These genes are also highly expressed in normal PC samples. The scale of the gene expression data is similar to Fig. 1A.
- Figure S3. Expression of cell cycle related genes in normal B cells, CLL, WM, MM and normal PCs (JPG, 46.9 KB)
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The pattern of expression of these genes was very similar between normal B cells and CLL. Most of the genes encoding cyclin D kinaseinhibitors were upregulated in the malignant cells (especially in MM and WM) compared to the normal B cells or PCs. The scale of the gene expression data is similar to Fig. 1A.
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