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Blood, Vol. 108, Issue 9, 3045-3052, November 1, 2006
Continuous signaling via PI3K isoforms  and  is required for platelet ADP receptor function in dynamic thrombus stabilization
Blood Cosemans et al.
108: 3045
Supplemental material for: Cosemans et al
Files in this Data Supplement:
- Video 1. Thrombi remain stable during post-perfusion with plasma (WMV, 823 KB)
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Human blood was perfused over a vWF/collagen coated surface at a shear rate of 1000 s-1 for 4 min. Thrombi formed on collagen, were then post-perfused for 10 min with PPACK-anticoagulated plasma, while phase contrast images were captured.
- Video 2. Complete disintegration of thrombi during post-perfusion with HEPES buffer (WMV, 979 KB)
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Human thrombi were formed on vWF/collagen, as described for movie A. Post-perfusion for 10 min was with HEPES buffer pH 7.45 containing 2 mM CaCl2 and 2 mM MgCl2.
- Video 3. Key role of P2Y12 in thrombus stability in the presence of coagulation (WMV, 1.84 MB)
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Citrated human blood was recalcified with CaCl2/MgCl2 before flowing over a vWF/collagen surface at a shear rate of 1000 s-1. Fibrin-containing thrombi formed on collagen were post-perfused for 10 min with HEPES buffer pH 7.45, containing 2 mM CaCl2 and 2 mM MgCl2, supplemented with AR-C69931MX (30 µM).
- Video 4. ADP prevents disintegration of thrombi in the presence of coagulation (WMV, 1.85 MB)
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Citrated human blood was recalcified with CaCl2/MgCl2 before flowing over a vWF/collagen surface at a shear rate of 1000 s-1. Fibrin-containing thrombi on coverslips were post-perfused for 10 min with HEPES buffer pH 7.45, containing 2 mM CaCl2 and 2 mM MgCl2, supplemented with ADP (20 µM).
- Video 5. Absence of PI3Kγ leads to the formation of unstable embolizing thrombi (AVI, 4.71 MB)
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Formation of thrombi during flow over a vWF/collagen surface at 1000 s-1 for 4 min, recorded by high-speed fluorescence imaging. Blood from PI3K -/- mice was labeled with non-blocking AF5-568-anti-GPIb mAb Xia.B2.
- Video 6. Stable thrombus formation with blood from wildtype mice (AVI, 4.14 MB)
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Formation of thrombi during flow over a vWF/collagen surface at 1000 s-1 for 4 min, recorded by high-speed fluorescence imaging. Blood from wildtype mice was labeled with non-blocking AF5-568-anti-GPIb mAb Xia.B2.
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