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Blood, Vol. 109, Issue 7, 2944-2952, April 1, 2007
Twisted gastrulation (Tsg) is regulated by Tob and enhances TGF-ß signaling in activated T lymphocytes
Blood Tzachanis et al.
109: 2944
Supplemental materials for: Tzachanis et al, Vol 109, Issue 7, 2944-2952
Files in this Data Supplement:
- Figure S1. Results with a commercial Tsg (PDF, 11.9 KB) -
Primed alloreactive CD4+ T-cell lines were cultured as in Figure 3, except that commercially available recombinant Tsg (R&D Systems) was used. Proliferation was determined by 3H-thymidine incorporation for the last 16 hours of a 72-hour total period of culture, and IL-2 production was determined by ELISA in the supernatants of 24-hour cultures.
- Figure S2. Use of subinhibitory concentrations of TGF-β (PDF, 11.4 KB) -
Primed alloreactive CD4+ T cell lines were cultured with anti-CD3 mAb as in Figure 3, in the presence of subinhibitory concentrations of TGF- (0.25 ng/mL) in the absence or in the presence of increasing concentrations of Tsg. Proliferation was determined by 3H-thymidine incorporation for the last 16 hours of a 72-hour total period of culture.
- Figure S3. Tsg interacts with TGF-β (PDF, 58.1 KB) -
Purified hTGF-–V5 and mTsg (R&D Systems) were incubated overnight at 4°C, and subsequently immunoprecipitation was done with V5-specific antibody coupled on protein G. Complexes (lanes 1-2) were analyzed by SDS-PAGE along with purified proteins (lanes 3-4), and immunoblot was conducted using anti-Tsg mAb or anti-V5 mAb. Incubation of purified hTGF-–V5 with diluent used to reconstitute Tsg protein was used as control (indicated as −).
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