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Blood, Vol. 108, Issue 9, 3143-3151, November 1, 2006
MMP-9 in B-cell chronic lymphocytic leukemia is up-regulated by  4 1 integrin or CXCR4 engagement via distinct signaling pathways, localizes to podosomes, and is involved in cell invasion and migration
Blood Redondo-Muñoz et al.
108: 3143
Supplemental materials for: Redondo-Muñoz et al
Files in this Data Supplement:
- Supplemental Methods (PDF, 72 KB)
- Figure S1. Viability of B-CLL cells upon incubation on BSA or H89 fragment for 24 h (JPG, 31.4 KB)
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B-CLL cells from 2 representative samples were added to BSA- or FN-H89-coated wells; after 24 h, cell viability was determined by flow cytometry using Annexin V-FITC and propidium iodide. Numbers indicate the percentage of viable cells for each condition.
- Figure S2. Viability of B-CLL cells after incubation with various protein kinase inhibitors for 24 h (JPG, 44.8 KB)
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B-CLL cells from a representative patient were pre-incubated for 1 h with either 5 µM UO126, 30 nM wortmannin (Wmn), 1 µM API-2, or 5 µM LY294002 (LY), and added to plates coated with FN-H89 (10 µg/ml). Control cells were pre-incubated with medium alone. After 24 h, cell viability was determined by blow cytometry using Annexin V-FITC and propidium idodide. Numbers represent the percentage of viable cells for each condition.
- Figure S3. Kinetic analysis of IκB-α expression during adhesion of B-CLL cells to the H89 fragment (JPG, 37.2 KB)
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B-CLL cells from a representative patient were added to BSA- or FN-H89-coated plates. At the indicated times, cells were lysed and lysates analysed by western blotting using anti-I B- mAbs. Values represent the relative IB- levels for each condition, after normalization with respect to actin levels.
- Figure S4. Viability of B-CLL cells after transfection with siRNAs for MMP-9 (JPG, 41.2 KB)
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B-CLL cells from a representative patient were transfected con a control siRNA or with 3 different siRNA for MMP-9. After 24 h, the concentration of FCS was reduced to 0.1% (same conditions used for the functional assays described in Figure 6C and D of the main manuscript) and cells incubated for an additional 24 h period. Cells were then analysed by flow cytometry using Annexin V-FITC and propidium iodide. Values indicate the percentage of viable cells for each condition.
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