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Blood, Vol. 108, Issue 13, 4025-4034, December 15, 2006
Ikaros is required for plasmacytoid dendritic cell differentiation
Blood Allman et al.
108: 4025
Supplemental materials for: Allman et al, Vol 108, Issue 13, 4025-4034
Files in this Data Supplement:
- Table S1. Complete list of up-regulated genes in IkL/L BM pDCs (PDF, 145 KB) -
Complete list of genes that were increased in all 9 IkL/L/WT comparisons. Fold changes correspond to the average of 9 values. The last column indicates the number of independent probe sets (when > 1) detecting the same gene, with similar variations between WT and mutant samples.
- Table S2. Complete list of down-regulated genes in IkL/L BM pDCs (PDF, 99.8 KB) -
Complete list of genes that were decreased in all 9 IkL/L/WT comparisons. Fold changes correspond to the average of 9 values. The last column indicates the number of independent probe sets (when > 1) detecting the same gene, with similar variations between WT and mutant samples.
- Figure S1. cDC responses to MCMV and STAg stimulation (JPG, 128 KB)
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(A) Mice were infected with MCMV as described in Figure 3. Spleen DC subsets were analyzed 1.5 days after challenge for IL-12, CD86, and MHC class II (I-Ab) expression. Spleen cells were first distinguished on the basis of CD11c and 120G8 expression. pDCs were defined as CD11clo120G8+ cells. CD11c+120G8− cDCs were further separated into CD8 + and CD8− cDC subsets and analyzed for IL-12 expression. Histograms show CD86 and I-Ab expression of total cDCs. Gray histograms indicate expression on day 0 cells; white histograms indicate expression on day 1.5 cells. Results shown are representative of 2 experiments, with 3 to 8 mice/genotype per condition. NA indicates not applicable. (B) Mice were injected intraperitoneally with 10 µg STAg in 200 µL PBS. Six hours after injection, spleen DC subsets were analyzed as described in panel A. Gray histograms indicate expression in cells injected with PBS; white histograms indicate expression in cells injected with STAg. Results are representative of 3 experiments.
- Figure S2. Cellular localization of deregulated gene products (JPG, 44.8 KB)
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Gene ontology annotations for the top 150 up-regulated and 50 down-regulated genes were retrieved from the NetAffx Analysis Center. Genes with known cellular localizations were grouped into 4 main classes: extracellular, membrane, cytoplasmic and intracellular organelles, and nucleus. Note that the genes that code for surface or secreted proteins account for two thirds of the annotated genes.
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