|
|
Blood, Vol. 109, Issue 2, 819-826, January 15, 2007
Rapid quantification of naive alloreactive T cells by TNF- production and correlation with allograft rejection in mice
Blood Brehm et al.
109: 819
Supplemental materials for: Brehm et al, Vol 109, Issue 2, 819-826
Files in this Data Supplement:
- Figure S1. Naive, effector, and nonresponsive H2k-specific CD8+ T cells distinguished by differential expression of cytokines and activation markers (JPG, 90.3 KB)
-
Splenocytes from naive C57BL/6 mice, C57BL/6 mice that were injected with CBA splenocytes (CBA, H2k) 7 days previously, or C57BL/6 mice that were tolerized with anti-CD154 mAb and CBA DST were stimulated in vitro with either (A) CBA (H2k) splenocytes or (B) BALB/c (H2d) splenocytes. Samples were then stained for cell-surface markers and for intracellular cytokines. Samples were gated on CD8+ cells, and the values shown represent the percentage of CD8+ T cells staining positive for TNF- . (C) H2k-specific CD8+ T cells producing TNF- were evaluated for IFN- production, and the values represent the percentage of TNF-–producing CD8+ T cells that were positive for IFN-. The total number of CD8+ T cells producing TNF- following stimulation with CBA (H2k) splenocytes is shown in panel D (n = 4). One-way analysis of variance (ANOVA) was used to compare naive C57BL/6 mice with mice treated with DST or with mice treated with DST and MR1 (*P < .05; **P < .05). The data are representative of 2 experiments
|
|
