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Blood, Vol. 109, Issue 1, 339-342, January 1, 2007
JAK2V617F: prevalence in a large Chinese hospital population
Blood Xu et al.
109: 339
Supplemental materials for: Xu et al, Vol 109, Issue 1, 339-342
Files in this Data Supplement:
- Figure S1. Detection of JAK2V617F by BsaX1 restriction enzyme digestion (PDF, 482 KB) -
Blood genomic DNA samples were amplified by PCR with P1 and P1r primers, and the 521-bp PCR product was gel purified. (A) The purified PCR products were digested with BsaX1 for 3 hours and then analyzed on 3% agarose gel. (B) The BsaX1 digests were further amplified by allele-specific PCR with primers P2 and Pmr, and then PCR products were analyzed on 3% agarose gel. Lanes +, –, and M denote positive control, negative control, and molecular markers, respectively. The expected positive bands were indicated by arrows. Note that allele-specific PCR following BsaX1 digestion is much more sensitive and thus allows detection of more positive samples.
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