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Blood, Vol. 108, Issue 8, 2850-2856, October 15, 2006
Non-side-population hematopoietic stem cells in mouse bone marrow
Blood Morita et al.
108: 2850
Supplemental materials for: Morita et al
Files in this Data Supplement:
- Table S1. SP and MP cells among various populations (PDF, 8.94 KB) -
Data are expressed as mean±S.D. (n=7). Unpaired t testing showed P=0.0053 for comparison of %SP cells among Lin- cells and %SP cells among KSL cells, and P<0.0001 for comparison of %SP cells among Lin- cells and %SP cells among CD34-KSL cells.
- Table S2. CD34-KSL cells among various populations (PDF, 8.98 KB) -
Data are expressed as mean±S.D. (n=7). Unpaired t testing showed P<0.0001 for comparison of %CD34-KSL cells among Lin- cells and %CD34-KSL cells among SP- Lin- cells, and P=0.0102 for comparison of %CD34-KSL cells among Lin- cells and %CD34-KSL cells among MP-Lin- cells.
- Table S3. SP- and MP-CD34-KSL cells among lineage marker- cells (PDF, 8.89 KB) -
Data are expressed as mean±S.D. (n=7). Unpaired t testing showed P=0.7116 for comparison of %SP-CD34-KSL cells and %MP-CD34-KSL cells.
- Table S4. Undetectable repopulating activity in SP-CD34+KSL cells (PDF, 9.13 KB) -
5 SP-CD34-KSL (CD34-) cells or SP-CD34+KSL (CD34+) cells were transplanted into 10 lethally irradiated mice, together with 2×105 competitor cells. Peripheral blood cells of the recipient mice were analyzed 4 months after transplantation. The number of engrafted mice per the number of transplanted mice is shown. The percent chimerism is shown as mean ± S.D. for total leukocytes, myeloid cells, B-lymphoid cells, and T-lymphoid cells.
- Figure S1. Reanalysis results of sorted cells (PDF, 201 KB) -
After SP-CD34-KSL cells and MP-CD34-KSL cells were individually sorted, a portion of sorted cells was analyzed to examine their purity. (A) Reanalysis of sorted SP-CD34-KSL cells. (B) Reanalysis of sorted MP-CD34-KSL cells.
- Figure S2. BrdU uptake by SP- and MP-CD34-KSL cells (PDF, 258 KB) -
After administration of BrdU to mice for 3 weeks, 5,000 SP- or MP-CD34-KSL cells were isolated and analyzed for BrdU incorporation and DNA content on a flow cytometer.
- Figure S3. CD34 expression in SP-KSL cells (PDF, 227 KB) -
(A) SP cells are gated among Lin-BM cells. (B) Kit+Sca-1+ cells are gated among Lin-SP cells. (C) The sorting gates for SP-CD34-KSL cells and SP-CD34+KSL cells are shown.
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