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Blood, Vol. 108, Issue 7, 2290-2299, October 1, 2006
Granzyme B, a novel mediator of allergic inflammation: its induction and release in blood basophils and human asthma
Blood Tschopp et al.
108: 2290
Supplemental materials for: Tschopp et al
Files in this Data Supplement:
- Table S1 and Methods (PDF, 21.8 KB)
- Figure S1. IL-3-induced Granzyme B expression in human basophils isolated from the blood of 9 different healthy volontiers (JPG, 41.8 KB)
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Basophils were purified from the blood of healthy donors which had no history of symptoms of allergic disease (rhino-conjunctivitis, asthma, atopic dermatitis). Basophils were cultured during 24 hours in medium without (circles) or with (triangles) the addition of a maximally effective concentration of IL-3 (10 ng/ml). Cells were lysed in culture medium without centrifugation and the total amounts of granzyme B was measured by ELISA. Symbols represent the mean value of triplicates of each donor. The means and SEM are shown by thick or thin lines, respectively.
- Figure S2. Tryptase, IL-13, Granzyme B and Granzyme A levels 10 minutes and 18 hours after semental provocation (JPG, 52.8 KB)
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See legend figure 7. Mean (line) and individual values (symbols) of the different experimental groups for Tryptase, IL-13, GzmB and GzmA levels in the BAL of asthma patient after segmental challenge are shown.
- Figure S3. Tryptase, IL-13, Granzyme B and Granzyme A levels in BAL-fluids 10 minutes and 42 or 162 hours after provocation (JPG, 55.7 KB)
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Segmental allergen provocation was performed in 10 patients suffering from allergic asthma. Separate lung segments were challenged with saline or antigen and bronchiolar-alveolar lavage (BAL) was performed in the same session 10 min later. 42 h (left) or 162 h (right) after challenge a second bronchoscopy was performed and BAL was repeated. Levels of tryptase, IL-13, granzyme B and granzyme A in the BAL samples of the different patients are shown.
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