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Blood, Vol. 108, Issue 10, 3560-3563, November 15, 2006
Overexpression of CEBPA resulting from the translocation t(14;19)(q32;q13) of human precursor B acute lymphoblastic leukemia
Blood Chapiro et al.
108: 3560
Supplemental materials for: Chapiro et al, Vol 108, Issue 10, 3560-3563
Files in this Data Supplement:
- Table S1. Immunophenotypes of BCP-ALL (PDF, 22.3 KB)
- Figure S1. Partial map of the chromosome 19 and 14 region involved in the t(14;19) translocation (JPG, 28.3 KB)
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Top panel shows map of the 19q13 region. The BAC probe RP11-270I13 produces a split signal in all patients with BCP-ALL who have t(14;19). The vertical arrow indicates the location of the breakpoint in patients P1 and P2. Bottom panel shows map of the IGH gene around the Cmu-JH-DH region. The breakpoints of P1 and P2 occur within the JH cluster. Exons appear as empty boxes and the enhancer as a black dot. The map is not drawn to scale.
- Figure S2. Expression analysis of CEBPG, LRP3, and PEPD genes (JPG, 43.8 KB)
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Quantitative RT-PCR analyses of 3 genes surrounding the chromosome 19 breakpoint in t(14;19) samples and 3 patients with BCP-ALL devoid of chromosome 19 abnormality. Data are shown in comparison to ABL expression in the samples. Note that the scale differs from Figure 1. The reference of the gene expression assay is indicated.
- Figure S3. Western blot detection of CEBPA proteins (JPG, 22.3 KB)
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Detection of CEBPA protein species by Western blot analyses using a goat anti-CEBPA immuneserum (sc 9314; Santa Cruz Biotechnology). Arrowheads indicate the known CEBPA p42 and p30 protein species. U937 and MOLT-4 extracts were loaded as positive and negative control, respectively. Left panel shows 2 × 106 cells directly lysed in Laemmli buffer and loaded in the gel. Right panel shows that the p30 CEBPA species is not observed, because only 100 µg of whole-cell extract was loaded for P2 and P4.
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