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Blood, Vol. 108, Issue 13, 4018-4024, December 15, 2006
Primitive erythropoiesis from mesodermal precursors expressing VE-cadherin, PECAM-1, Tie2, endoglin, and CD34 in the mouse embryo
Blood Ema et al.
108: 4018
Supplemental materials for: Ema et al, Vol 108, Issue 13, 4018-4024
Files in this Data Supplement:
- Table S1. Cell-surface expression of PECAM-1, VE-cadherin, and Tie2 on cells expressing GFP (PDF, 94 KB) -
Data represent the means ± SD of 3 independent experiments. For each experiment, 10 embryos (LS-OB) were pooled and treated with collagenase and then were incubated with a combination of antibodies.
- Figure S1. Whole-mount antibody staining of embryos using the anti-Flk1, VE-cadherin, Tie2, and endoglin antibodies (JPG, 73.9 KB)
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Asterisk indicates endoglin expression at the extraembryonic ectoderm. ex indicates extraembryonic region; em, embryonic region; VE, visceral endoderm.
- Figure S2. Gastrulating embryos express Flk1, PECAM-1, and GATA1 in the extraembryonic mesoderm layer (JPG, 57.9 KB)
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Boxed areas in Figure 3E,J,N are magnified. Images before merge are also shown.
- Figure S3. Flow cytometric analysis of wild-type embryos at late streak–to–no bud stage (LS-OB) with cell surface markers VE-cadherin and PECAM-1 (JPG, 41.6 KB)
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