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Blood, Vol. 108, Issue 6, 1868-1876, September 15, 2006
Thrombin-induced endothelial microparticle generation: identification of a novel pathway involving ROCK-II activation by caspase-2
Blood Sapet et al.
108: 1868
Supplemental materials for: Sapet et al
Files in this Data Supplement:
- Table S1. Genes expression changes induced by thrombin (PDF, 94.1 KB) -
The table represents 157 genes up-regulated and 31 genes down-regulated determined by the unsupervised hierarchical clustering and differential expression as described in “Material and Methods.” The values represent fold changes in gene expression induced by thrombin compared to untreated cells. Genes were clustered into functional families according to their biological process in Gene Ontology and BIOSOURCE database.
- Figure S1A. Microarray analyses of thrombin-stimulated HMEC-1:
total tree presenting up- and downregulated patterns (JPG, 82.8 KB)
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Total cellular RNA from three independent experiments was isolated from unstimulated or thrombin stimulated cells at 0.5, 1, 2, 4, 6, 12 and 18 hours. 3µg of the RNA was converted into double-stranded CRNA by reverse transcription, labeled with 33PdCTP and hybridized on custom nylon chips containing 7874 selected IMAGE human cDNA clones and 200 cDNA controls as described in Materials and Methods. The images from the scanned chips were processed by means of Cluster and Tree-wiew softwares as described by Eisen (ref). The figure shows a supervised hierarchical clustering of gene expression in HMEC-1 in response to thrombin (1.0 IU/ml). After data filtering, patterns of genes with at least a two-fold variation are represented. Only genes present in the three independent experiments were considered. The mean relative expression levels for genes in HMEC-1 are presented by a color scale, whereby red reflects high expression (2 × SD) and green reflects low expression (-2 × SD). Distances between nodes of the tree diagram indicate the degree of association between genes or groups of genes. Correlated genes are grouped across the samples into clusters localized by the colored boxes. A: total tree presenting up- and downregulated patterns; Genes upregulated at B: 0.5, 1 and 2 hours; C: 4, 6 and 12 hours D: downregulated genes at 1 and 4 hours.
- Figure S1B. Genes upregulated at 0.5, 1 and 2 hours (JPG, 280 KB)
- Figure S1C. Genes upregulated at 4, 6 and 12 hours (JPG, 204 KB)
- Figure S1D. Genes downregulated at 1 and 4 hours (JPG, 61.5 KB)
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