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Blood, Vol. 108, Issue 9, 3085-3093, November 1, 2006
Hypogammaglobulinemia and exacerbated CD8 T-cell–mediated immunopathology in SAP-deficient mice with chronic LCMV infection mimics human XLP disease
Blood Crotty et al.
108: 3085
Supplemental materials for: Crotty et al, Vol 108, Issue 9, 3085-3093
Files in this Data Supplement:
- Figure S1. CD8 and CD4 T cells, day 30 after infection with LCMVcl13 (PDF, 251 KB) -
Wild-type and SAP– mice were infected with LCMVcl13. T-cell responses were quantified at day 30 after infection. (A) Percentage of LCMV-specific (gp33-41 Db epitope) CD8 T cells was comparable between SAP– and B6 mice (P > 0.05), although absolute numbers were lower in SAP– mice (data not shown) due to smaller spleen size (Figure 6). (B) Intracellular cytokine staining of CD8 T cells for IFN (top) and TNF (bottom) after stimulation with gp33-41 peptide. CD8+ gated cells are shown. (C) Percentage of LCMV-specific (gp61-80 I-Ab epitope) CD4 T cells was comparable between SAP– and B6 mice (P > 0.05), although absolute numbers were lower in SAP– mice (data not shown) due to smaller spleen size (Figure 6). LCMV-specific CD4+ T cells in spleen were quantified by 5 hours of stimulation with the immunodominant gp61-80 I-Ab MHCII peptide and were then analyzed by intracellular cytokine staining for IFN production (WT, n = 7; SAP–, n = 3). (D) Intracellular cytokine staining of CD4 T cells for IL-2 and IFN after stimulation with gp61-80 peptide. CD4+ gated cells are shown. Data are representative of 3 independent experiments.
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