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Blood, Vol. 108, Issue 7, 2463-2469, October 1, 2006
Trans-presentation of donor-derived interleukin 15 is necessary for the rapid onset of acute graft-versus-host disease but not for graft-versus-tumor activity
Blood Blaser et al.
108: 2463
Supplemental materials for: Blaser et al
Files in this Data Supplement:
- Figure S1. Absence of donor-derived IL-15 does not decrease donor T-cell chimerism (JPG, 64.7 KB)
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Lethally irradiated wt B6D2F1 mice were transplanted with 5 × 106 wt B6 splenic T cells and 1 × 107 TCD BM cells from either wt B6 mice or IL 15-/- B6 mice, sacrificed at the time points indicated, and spleens harvested. Chimerism was calculated by dividing the number of CD4+H-2Dd- or CD8+H-Dd- events by the total number of CD4+ or CD8+ events. Values for individual mice from 1 of 3 to 5 similar experiments were averaged and plotted as mean ± s.e.m. The total number of mice analyzed in each group at each time point was between 9 and 14. There was no statistically significant decrease in T cell chimerism at any time point analyzed in recipients of IL-15-/- B6 BM cells.
- Figure S2. Absence of donor-derived IL-15 does not decrease reconstitution of donor-derived CD8+ CD122+ memory T cells (JPG, 51.2 KB)
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Lethally irradiated wt B6D2F1 mice were transplanted with 5 × 106 wt B6 splenic T cells and 1 × 107 TCD BM cells from either wt B6 mice or IL 15-/- B6 mice, sacrificed at the time points indicated, and spleens harvested. The number of CD8+ CD122+ H-2Dd- events was divided by the total viable cell events collected and multiplied by the number of splenocytes recovered for each mouse. Values for individual mice from 1 of 3 to 4 similar experiments were averaged and plotted as mean ± s.e.m. The total number of mice analyzed in each group at each time point was between 9 and 14. There was no statistically significant decrease in memory T cell reconstitution at any time point analyzed in recipients of IL-15-/- B6 BM cells.
- Figure S3. Absence of donor-derived IL-15 does not decrease reconstitution of donor-derived CD8+ CD44hi CD62Llo effector memory T cells (JPG, 51.1 KB)
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Lethally irradiated wt B6D2F1 mice were transplanted with 5 × 106 wt B6 splenic T cells and 1 × 107 TCD BM cells from either wt B6 mice or IL 15-/- B6 mice, sacrificed at the time points indicated, and spleens harvested. The number of CD8+ CD44hi CD62Llo H-2Dd- events was divided by the total viable cell events collected and multiplied by the number of splenocytes recovered for each mouse. Values for individual mice from 1 of 3 to 4 similar experiments were averaged and plotted as mean ± s.e.m. The total number of mice analyzed in each group at each time point was between 9 and 14. There was no statistically significant decrease in effector memory T cell reconstitution at any time point analyzed in recipients of IL-15-/- B6 BM cells.
- Figure S4. Absence of donor-derived IL-15 does not decrease reconstitution of donor-derived CD8+ CD44hi CD62Lhi central memory T cells (JPG, 51.7 KB)
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Lethally irradiated wt B6D2F1 mice were transplanted with 5 × 106 wt B6 splenic T cells and 1 × 107 TCD BM cells from either wt B6 mice or IL 15-/- B6 mice, sacrificed at the time points indicated, and spleens harvested. The number of CD8+ CD44hi CD62Lhi H-2Dd- events was divided by the total viable cell events collected and multiplied by the number of splenocytes recovered for each mouse. Values for individual mice from 1 of 3 to 4 similar experiments were averaged and plotted as mean ± s.e.m. The total number of mice analyzed in each group at each time point was between 9 and 14. There was no statistically significant decrease in central memory T cell reconstitution at any time point analyzed in recipients of IL-15-/- B6 BM cells.
- Figure S5. Absence of donor-derived IL-15 does not decrease chimerism or reconstitution of donor-derived NK cells (JPG, 61.6 KB)
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Lethally irradiated wt B6D2F1 mice were transplanted with 5 × 106 wt B6 splenic T cells and 1 × 107 TCD BM cells from either wt B6 mice or IL 15-/- B6 mice, sacrificed at the time points indicated, and spleens harvested. Chimerism was calculated by dividing the number of NK1.1+ CD3- H-2Dd- events by the total number of NK1.1+ CD3- events. Absolute numbers of donor-derived NK cells were calculated by dividing the number of NK1.1+ CD3- H-2Dd- events by the total viable cell events collected and multiplying by the number of splenocytes recovered for each mouse. Values for individual mice from 1 of 2 similar experiments were averaged and plotted as mean ± s.e.m. The total number of mice analyzed in each group at each time point was between 4 and 7. There was no statistically significant decrease in memory NK cell reconstitution at any time point analyzed in recipients of IL-15-/- B6 BM cells.
- Figure S6. Absence of donor-derived IL-15 does not decrease myeloid reconstitution (JPG, 80.7 KB)
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Lethally irradiated wt B6D2F1 mice were transplanted with 5 × 106 wt B6 splenic T cells and 1 × 107 TCD BM cells from either wt B6 mice or IL 15-/- B6 mice, sacrificed at the time points indicated, and spleens harvested. Absolute numbers of donor-derived monocytes/granulocytes (CD11b+ CD11c- H-2Dd-) and dendritic cells (CD11b- CD11c+ H-2Dd-) were calculated by dividing the number of monocyte/granulocyte or dendritic cell events by the total viable cell events collected and multiplying by the number of splenocytes recovered for each mouse. Values for individual mice from 1 of 2 to 3 similar experiments were averaged and plotted as mean ± s.e.m. The total number of mice analyzed in each group at each time point was between 5 and 13. There was no statistically significant decrease in monocyte/granulocyte or dendritic cell reconstitution at any time point analyzed in recipients of IL-15-/- B6 BM cells.
- Figure S7. Absence of donor-derived IL-15 does not alter IEL phenotype (JPG, 107 KB)
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Lethally irradiated wt B6D2F1 mice were transplanted with 5 × 106 wt B6 splenic T cells and 1 × 107 TCD BM cells from either wt B6 mice or IL 15-/- B6 mice, sacrificed at the time points indicated, and gut (duodenum to rectum) was harvested. IEL were harvested as described in Methods and phenotyped by flow cytometry. Data represent mean ± s.e.m. from 1 of 2 similar experiments. Between 4 and 6 mice were analyzed at each time point. There was no statistically significant difference in IEL phenotype at any time point analyzed.
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