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Blood, Vol. 109, Issue 2, 836-842, January 15, 2007
Attenuation of phagocytosis of xenogeneic cells by manipulating CD47
Blood Wang et al.
109: 836
Supplemental materials for: Wang et al, Vol 109, Issue 2, 836-842
Files in this Data Supplement:
- Figure S1. Clearance of CD47 KO splenocytes in WT mice (PDF, 22.1 KB) -
(A-B) PKH26-labeled WT and CFSE-labeled CD47 KO mouse spleen cells were mixed at a ratio of 1:1, and intravenously injected into WT (A; n = 3) or CD47 KO (B; n = 3) mice (total 5 × 107 per mouse). Mice were bled at 2, 8, 24, 48, and 72 hours after cell infusion, and the percentages of injected cells in white blood cells (WBCs) were determined by flow cytometric analysis. Data shown are percentages (mean ± SDs) of injected WT (●) and CD47 KO (○) splenocytes, which were normalized with the levels at 2 hours after cell transfer as 100%. (C-D) PKH26-labeled WT and CFSE-labeled CD47 KO mouse spleen cells were mixed at a ratio of 1:1, and intravenously injected into WT mice (total 5 × 107 per mouse; n = 3). Mice were bled at 2, 4, 8, 24, and 48 hours after cell infusion; WBCs were prepared and stained with APC-conjugated anti–T-cell (TCR  ) or anti–B-cell (B220) mAbs, and the percentages of injected T and B cells were analyzed by flow cytometry. Shown are percentages (mean ± SDs) of injected WT (●) and CD47 KO (○) T (C) and B (D) cells, which were normalized with the levels at 2 hours after cell transfer as 100%. Together, these results indicate that CD47 KO mouse splenocytes, including T and B cells, are rapidly cleared by macrophages in WT mice, but not in CD47 KO mice.
- Figure S2. CD47 KO and WT mice have a similar number of F4/80+ macrophages in the spleen (PDF, 12.7 KB) -
Spleen cells prepared from 12-week-old WT (n = 3) and CD47 KO (n = 3) mice were stained with R-phycoerythrin (R-PE)–conjugated antimouse F4/80 (Caltag Laboratories, Burlingame, CA), and the percentages of F4/80+ macrophages were determined by flow cytometric analysis. (A) Percentages (mean ± SDs) of F4/80+ cells in the spleen. (B) Numbers (mean ± SDs) of F4/80+ cells per spleen.
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