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Blood, Vol. 110, Issue 6, 1739-1747, September 15, 2007
Lipid rafts are required for Kit survival and proliferation signals
Blood Jahn et al.
110: 1739
Supplemental materials for: Jahn et al, Vol. 110, Issue 6, 1739-1747
Files in this Data Supplement:
- Figure S1. SFKs are constitutively present and highly enriched in lipid rafts of Mo7e cells (JPG, 62.1 KB)
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Subcellular fractions, as in Figure 1A, were immunoblotted with specific antibodies against the SFKs Hck, Lck, Fyn, and Lyn, against the phospho-tyrosine residues(PY), and against the non-SFK tyrosine kinase Syk.
- Figure S2. Distribution of tyrosine-phophorylated proteins in Mo7e cells (JPG, 52.5 KB)
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Subcellular fractions, as in Figure 1A, were separated by 10% SDS-PAGE and immunoblotted against PY. Phosphorylated Kit (closed arrow) and pSFKs (bracket) in lipid rafts are indicated.
- Figure S3. Effect of imatinib treatment of HMC-1 cells on the presence of SFKs in lipid rafts (JPG, 57.8 KB)
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Subcellular fractions, as in Figure 2A, were immunoblotted with specific antibodies against the SFKs Hck, Lck, Fyn, Lyn, and PY.
- Figure S4. Distribution of tyrosine-phophorylated proteins in HMC-1 cells (JPG, 56.6 KB)
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Subcellular fractions as in Figure 2A were separated by 7.5% SDS-PAGE and immunoblotted against PY. Phosphorylated Kit (closed arrow) and pSFKs (bracket) in lipid rafts are indicated.
- Figure S5. Comparison of Mo7e cell viability after 20 hours incubation in media alone or media with the indicated concentrations of MβCD (JPG, 26.5 KB)
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To test for nonspecific cell death, Mo7e cells were incubated over the time period of 20 hours either in media alone or in media containing 2 or 4 mM M CD. Cells were washed, Trypan blue stained, and viable cells were counted. Error bars represent the range of cell numbers obtained in three experiments.
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