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Blood, Vol. 109, Issue 3, 1113-1122, February 1, 2007
Dendritic-cell maturation alters intracellular signaling networks, enabling differential effects of IFN- /ß on antigen cross-presentation
Blood Longman et al.
109: 1113
Supplemental materials for: Longman et al, Vol 109, Issue 3, 1113-1122
Files in this Data Supplement:
- Table S1 (PDF, 64.4 KB) -
A. >2.5× in both iDCs + IFN and mDCs + IFN iDC + IFN & mDC + IFN signal value >100, present.
B. >2.5× in iDCs + IFN iDC + IFN signal value >100, present; <1.5× in mDCs + IFN.
C. >2.5× mDCs + IFN mDC + IFN signal value >100, present; <1.5× iDCs + IFN.
- Figure S1. IFNα/β early alters CD40L gene induction profile (PDF, 14.5 KB) -
Affymetrix data was queried for genes induced by CD40L that were inhibited by exposure of iDCs to IFN/ during maturation. The top nine differentially regulated genes are displayed graphically by relative expression in iDCs, mDC, or mDCs exposed to CD40L (mDC + 40L). Blue line, untreated. Red line, IFN/ treated.
- Figure S2. IFNα/β does not alter CD40L induced IκB-α phosphorylation or degradation (PDF, 24.9 KB) -
iDCs matured in the presence of 60 IU/ml IFN/, mDCs, and mDCs exposed to 60 IU/ml IFN/ were incubated with 0.5µg/mL CD40L for 30 minutes. 20µg of protein from the total cell lysates were run by 12% SDS-PAGE, transferred to PVDF membranes, and blotted with indicated antibodies as indicated in “Materials and Methods.”
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