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Blood, Vol. 108, Issue 9, 3035-3044, November 1, 2006
Blockade of  v 3 and v5 integrins by RGD mimetics induces anoikis and not integrin-mediated death in human endothelial cells
Blood Maubant et al.
108: 3035
Supplemental materials for: Maubant et al
Files in this Data Supplement:
- Figure S1. Effects of S 36578 2 on HUVEC spread on type I collagen (PDF, 63.7 KB) -
S 36578 2 was added to cells 3 hours after seeding onto type I collagen (5µg/cm2, BD Biosciences) in experimental medium. (A) Phase contrast micrographs were taken 24 hours after treatment. Scale bar, 100µm. Results from a representative experiment, repeated at least 3 times, are shown. (B) Pro caspase 8, pro-caspase 9, cleaved caspase 3 and PARP were detected by western blotting. Erk1/2 was used as loading control. Results from a representative experiment are shown (n=3).
- Figure S2. Time-dependent effects of S 36578 2 on HUVEC maintained in suspension (PDF, 12.4 KB) -
S 36578 2 was added to HUVEC at the time of seeding on polyHEMA. Floating cells were collected 6, 14 or 24 hours after treatment. Apoptosis was assessed by flow cytometry after annexin V and propidium iodide staining. One experiment out of two, with similar results, is shown.
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