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Blood, Vol. 109, Issue 3, 1138-1146, February 1, 2007
Distinct memory CD4+ T-cell subsets mediate immune recognition of Epstein Barr virus nuclear antigen 1 in healthy virus carriers
Blood Heller et al.
109: 1138
Supplemental materials for: Heller et al, Vol 109, Issue 3, 1138-1146
Files in this Data Supplement:
- Table S1. Synthetic peptides used in this study (PDF, 17.3 KB)
- Table S2. Frequency of CD4+ T cells expressing IFNγ (PDF, 16.7 KB)
- Table S3. Percent of proliferating CD4+ T cell precursors (PDF, 10 KB)
- Figure S1. A subpopulation of EBNA1 specific CD4+ T cells produced IL-2 in addition to IFNγ (PDF, 56 KB) -
IFN and IL-2 production was assessed after stimulation with the indicated stimuli (Medium: no stimulus, Staphyloccocus enteriotoxin B: SEB, MHC class I restricted CMV peptides: CMV, and subpool IV of EBNA1 peptides representing aa539-593: EBNA1*) by whole blood assay after gating on CD3+CD4+ T cells. The depicted values are frequencies of CD3+CD4+ T cells in the respective quadrants. A representative CD3+CD4+ T cell response from one out of 20 healthy volunteers is shown.
- Figure S2. EBV and CMV specific CD8+ T cell responses can be evaluated by intracellular cytokine staining (PDF, 48 KB) -
Whole blood assay gating on CD8+ T cells; value is the percentage of CD8+ T cells positive for IFN in response to Medium (no stimulus), Staphylococcus enterotoxin B (SEB), MHC class I restricted CMV peptides (CMV), and MHC class I restricted EBV peptides (EBV) (see Table S1). Representative CD8+ T cell responses from one out of 20 healthy volunteers are shown.
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