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Blood, Vol. 109, Issue 7, 2887-2893, April 1, 2007
Interferon regulatory factor 3-dependent responses to lipopolysaccharide are selectively blunted in cord blood cells
Blood Aksoy et al.
109: 2887
Supplemental materials for: Aksoy et al, Vol 109, Issue 7, 2887-2893
Files in this Data Supplement:
- Table S1. Complete list of differentially expressed genes in LPS-stimulated adult (Ad) versus neonatal (NB) moDCs (PDF, 36 KB)
- Table S2. Oligonucleotide sequences used for real-time RT PCR (PDF, 36.6 KB)
- Figure S1. Expression of MyD88, TRIF, TRAM, IRF-3, TBK1, and IKKε protein and TBK1 and IKKε mRNA in adult and neonatal moDCs (PDF, 40.4 KB) -
(A) Adult and cord blood moDCs (106 cells) were collected and directly lysed in Laemmli sample buffer. Equal volumes of total cell lysate were resolved by 8% SDS-PAGE and analyzed by Western blotting using antibodies directed against MyD88 (F-19; Santa Cruz Biotechnology), TRIF (ALX-210-908; Alexis Biochemicals, Zandhoven, Belgium), TRAM (ALX-210-914; Alexis Biochemicals), IRF-3 (550428; BD Biosciences, Erembodegem, Belgium), TBK1 (IMG-139; Imgenex, Huissen, The Netherlands), and IKK (IMG-270A; Imgenex). To control loading, immunoblots were reprobed with anti-GAPDH (Biodesign International, Venendaal, The Netherlands). Data are representative of 12 different donors in each group. (B) TBK-1 and IKK mRNA levels were determined in adult and cord blood moDCs by real-time RT-PCR.
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