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Blood, Vol. 109, Issue 9, 3786-3793, May 1, 2007
FcRL6, a new ITIM-bearing receptor on cytolytic cells, is broadly expressed by lymphocytes following HIV-1 infection
Blood Wilson et al.
109: 3786
Supplemental materials for: Wilson et al, Vol 109, Issue 9, 3786-3793
Files in this Data Supplement:
- Table S1. Antibodies used for flow cytometry (JPG, 91.6 KB)
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- Figure S1. FcRL6 does not significantly influence NK- or CD8+ T-cell cytotoxicity (JPG, 42.6 KB)
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(A) The presence or absence of anti-FcRL6 antibody has no effect in a redirected lysis assay using anti-CD16 as an activating stimulus. In addition, FcRL6 has minimal or no effect on cytotoxicity when primary NK cells are activated in the presence or absence of antibodies specific for 2B4, NKp30, DNAM-1, NKG2D, or CD11a (not shown). (B) NK92 cells transfected with wild-type FcRL6 show increased cytotoxicity in a redirected lysis assay in the presence of both anti-FcRL6 and anti-NKp30 compared with anti-NKp30 antibody alone. A similar increase in cytotoxicity is found in NK92 cells expressing a truncation mutant of FcRL6 that lacks the cytoplasmic tail, indicating that the increase in specific lysis is due to antibody-mediated adhesion between effector and target cells, and is not due to direct signaling by FcRL6. (C) The presence of anti-FcRL6 antibody has a negligible influence on anti-CD3 antibody-mediated redirected lysis of P815 target cells by purified CD8+ T cells.
- Figure S2. FcRL6 is not a prominent regulator of IFNγ or TNFα secretion (JPG, 50.4 KB)
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(A) Stimulation of primary NK cells overnight with IL-2 and plate-bound anti-FcRL6 F(ab) fragment leads to a slight increase in IFN and TNF production by primary NK cells. Graphs shown are the results from 4 separate donors with each value measured in triplicate. (B) Production of IFN and TNF by CD8+ T cells stimulated with plate-bound anti-CD3 is unaffected by FcRL6 stimulation.
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