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Blood, Vol. 109, Issue 6, 2406-2415, March 15, 2007
 4-Integrin+ endothelium derived from primate embryonic stem cells generates primitive and definitive hematopoietic cells
Blood Shinoda et al.
109: 2406
Supplemental materials for: Shinoda et al, Vol. 109, Issue 6, 2406-2415
Files in this Data Supplement:
- Figure S1. Single-cell deposition assay showing that VE-cadherin+CD45– cells contain single cells with hematopoietic and endothelial capacity (JPG, 108 KB)
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Single VE-cadherin+CD45– cells were sorted on day 10 and seeded onto fresh OP9 cells in 96-well plates. Seven days later, cells in each well were analyzed in situ by immunostaining with anti–VE-cadherin mAb (blue) and a mixture of anti-CD45, CD41, and  -globin mAbs (brown). Representative micrographs of wells containing endothelial progeny only (A), hematopoietic progeny only (B), or both endothelial and hematopoietic progeny (C) are depicted. Scale bar, 50 µm.
- Figure S2. The α4-integrin+ subpopulation among VE-cadherin+CD45– cells generates primitive and definitive erythrocytes sequentially (JPG, 65.4 KB)
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Immunostaining of floating erythrocytes on days 10+6 (A-E) and 10+30 (F-J) from VE-cadherin+ 4-integrin+ cells and on day 10+6 (K-O) from VE-cadherin–4-integrin+ cells. Cells were stained, as described for Figure 3. Merged images are shown. Scale bars, 50 µm.
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