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Blood, Vol. 109, Issue 4, 1568-1573, February 15, 2007 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef L-arginine availability regulates T-lymphocyte cell-cycle progression Blood Rodriguez et al. 109: 1568 Supplemental materials for: Rodriguez et al, Vol 109, Issue 4, 1568-1573 Files in this Data Supplement: Figure S1. L-Arg enhances expression and binding of E2F-1 to its consensus sequence (JPG, 44.7 KB) - (A) Stimulated T cells were cultured with or without L-Arg and cell extracts tested for E2F-1 expression. L-Arg replenishment (2 mM) was performed after 24 hours of L-Arg starvation. (B) Nuclear extracts of T cells cultured for 48 hours with or without L-Arg were tested for binding to radiolabeled E2F-1 consensus sequence. Exogenous cold probe, but not cold mutated probe, blocked the formation of the complex. Figure S2. Cyclin D3/cdk4 inhibitor expression in T cells cultured with and without L-Arg (JPG, 37.1 KB) - (A) Activated T cells (2 × 106) were cultured with or without L-Arg and tested for the expression of INK4 and KIP inhibitors. (B) Lysates from stimulated T cells cultured for 24 hours were prepared and 100 µg was immunoprecipitated with agarose-conjugated anti–cyclin-D3 or anti-p27Kip1 and then tested for p27Kip1. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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