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Blood, Vol. 109, Issue 6, 2565-2570, March 15, 2007
Hypoxia-inducible transcription factor–1 alpha determines sensitivity of endothelial cells to the proteosome inhibitor bortezomib
Blood Veschini et al.
109: 2565
Supplemental materials for: Veschini et al, Vol 109, Issue 6, 2565-2570
Files in this Data Supplement:
- Figure S1. MG132 and bortezomib induce death of HUVEC, mainly when HUVEC are grown in hypoxia (PDF, 11.6 KB) -
HUVEC were cultured either in normoxic or hypoxic (5% O2) conditions for 1 day, and where indicated, MG132 (7.5 mM) or bortezomib (100 nM) was added for the last 12 hours. The percentage of living cells, calculated as annexin V/PI double-negative, was assessed by FACS analysis. The MG132/bortezomib-induced apoptosis was greater when HUVEC were cultured in hypoxia compared with the normoxic/untreated counterpart. Data represent the mean plus or minus SEM of 3 independent experiments, and statistical analysis was performed with the Wilcoxon/Kruskal-Wallis test. **P < 0.5.
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