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Blood, Vol. 109, Issue 4, 1574-1583, February 15, 2007
Contribution of Toll-like receptors to the innate immune response to Gram-negative and Gram-positive bacteria
Blood Elson et al.
109: 1574
Supplemental materials for: Elson et al, Vol 109, Issue 4, 1574-1583
Files in this Data Supplement:
- Figure S1. Multiple cytokine bead array of conditioned supernatants from human whole blood stimulated by 104/mL K pneumoniae and 106/mL L monocytogenes (PDF, 53.5 KB) -
K pneumoniae is shown in panel A, and L monocytogenes is shown in panel B.
- Figure S2. Inhibitory effects of anti-TLR4, anti–MD-2, anti-TLR2, and anti-CD14 mAbs in human whole blood stimulated by heat-inactivated or antibiotic-inactivated E coli, K pneumoniae, P aeruginosa, and S aureus (PDF, 24.7 KB) -
Heat inactivation is shown by the black bars, and antibiotic inactivation by the white bars. Duplicates were performed for all conditions. The figure shows 1 of 2 representative experiments.
- Figure S3. Stimulation of whole blood and BEAS 2B cells with heat-inactivated whole bacteria (PDF, 46.6 KB) -
Bacteria were added to cells either with a wash step or not. (A) Whole blood (left) or BEAS 2B (right) was stimulated with the various bacteria at the concentration indicated. Cell stimulation was determined by the level of production of IL-6. (B) Either 104 cfu/mL E coli WT (left) or 106 cfu/mL L monocytogenes (right) were added to whole blood following incubation with the mAbs indicated. Results are expressed as a percentage of the IL-6 production relative to the control.
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