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Blood, Vol. 109, Issue 5, 2130-2138, March 1, 2007
SDX-308, a nonsteroidal anti-inflammatory agent, inhibits NF- B activity, resulting in strong inhibition of osteoclast formation/activity and multiple myeloma cell growth
Blood Feng et al.
109: 2130
Supplemental materials for: Feng et al, Vol. 109, Issue 5, 2130-2138
Files in this Data Supplement:
- Figure S1. Optimal inhibition of OCL formation requires 3-week culture with SDX drugs (PDF, 560 KB) -
Nonadherent cells from healthy donor (HBM) were cultured with 10 ng/mL M-CSF and 50 ng/mL RANKL for 21 days. DMSO (control; 0.1%), SDX-101 (75 µM), or SDX-308 (7.5 µM) was added to the culture twice a week. Drugs were added to (A) HBM or (C) MMBM only for the first week, the first 2 weeks, or all 3 weeks or to (B) HBM and (D) MMBM only for the last week, the last 2 weeks, or all 3 weeks. Then the cells were fixed and stained with 23c6 antibody to detect the multinucleated mature OCLs. Data shown are the mean ± SD of multinucleated cells (MNCs) per well of at least 8 wells. Asterisks indicate significant difference from control (P < .05). All experiments were performed independently 3 times.
- Figure S2. Densitometric analysis of phospho–IκB-α protein for Figure 6E (PDF, 284 KB) -
RAW cells were incubated with drug vehicle, SDX-101, or SDX-308 for 1 hour, treated with RANKL (100 ng/mL) for 30 minutes, and lysed. Phospho–I B- was detected in whole-cell lysates of RAW cells by Western blot analysis. Histogram shows the densitometric analysis of phosphorylated protein expression normalized to the equal loading (n = 3). Asterisks indicate significant difference from RANKL-treated control (P < .05).
- Figure S3. Densitometric analysis of nuclear p65 protein for Figure 7B (PDF, 283 KB) -
MM.1S MM cells were incubated with drug vehicle, SDX-101, or SDX-308 for 1 hour, treated with TNF- (20 ng/mL) for 15 minutes, and lysed. NE was prepared as described in Figure 7. NF-B p65 in NE was detected by Western blot assay. Histogram shows the densitometric analysis of nuclear p65 protein normalized to the equal loading (n = 3). Asterisks indicate significant difference from TNF-–treated control (P < .05).
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