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Blood, Vol. 109, Issue 4, 1381-1386, February 15, 2007
Shear flow–dependent integration of apical and subendothelial chemokines in T-cell transmigration: implications for locomotion and the multistep paradigm
Blood Schreiber et al.
109: 1381
Supplemental materials for: Schreiber et al, Blood, Vol 109, Issue 4, 1381-1386
These video files can be played using Microsoft Windows Media Player.
Files in this Data Supplement:
- Video 1. T cells cannot integrate apical and basal chemokine information under shear-free conditions (M1V, 3.91 MB)
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This is a representative movie for all trials performed under shear-free conditions in the presence of apical CXCL12 (10 ng/mL) and basal CCL5 (100 ng/mL). Eight minutes of a 10-minute trial are shown, compressed to 13.25 seconds. The green circle indicates a T cell characterized as locomotive (the dominant cell group in this arrangement). Note that the indicated cell travels across the endothelium over the course of the trial, encountering multiple paracellular junctions without undergoing TEM. Only cells that adhered to the endothelium before shear was stopped (at 3 minutes) were included in the analysis.
- Video 2. Shear flow enables adherent T-cells to integrate apical and basal chemokine information, promoting TEM (M1V, 4.05 MB)
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This is a representative movie for all trials performed under shear conditions in the presence of apical CXCL12 (10 ng/mL) and basal CCL5 (100 ng/mL). Most (8.5 minutes) of a 10-minute trial are shown, compressed to 14.17 seconds. The green circle indicates a T cell characterized as TEM (the dominant cell group in this arrangement). Note that the indicated cell arrests on the endothelium and completes TEM (note change in T-cell contrast to pale gray with passage through endothelium) within 5 minutes of time.
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