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Blood, Vol. 109, Issue 8, 3360-3368, April 15, 2007 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Vav proteins control MyD88-dependent oxidative burst Blood Miletic et al. 109: 3360 Supplemental materials for: Miletic et al, Vol 109, Issue 8, 3360-3368 Files in this Data Supplement: Figure S1. Dose-response of LPS and ROI production (JPG, 56.7 KB) - Peritoneal exudate cells from WT (C57/B6) mice were stimulated with varying doses of LPS, as indicated. Lucigenin chemiluminescence was used to detect ROI. RLU indicates relative light units. Figure S2. Peritoneal exudate cells from WT and Vavnull mice are phenotypically indistinguishable (JPG, 71.9 KB) - Mice were administered thioglycollate by intraperitoneal injection, and cells were harvested by peritoneal lavage 4 hours later. (A) Cell nuclei were stained with DAPI for microscopic examination. Percentages of WT and Vavnull cells with segmented nuclei were independently scored in triplicate and presented as the mean ± SD. (B) Cells were subsequently stained for the indicated markers and analyzed by FACS. Figure S3. Bone marrow–derived macrophages from WT and Vavnull mice are phenotypically indistinguishable (JPG, 61.4 KB) - Bone marrow was harvested and cultured in the presence of M-CSF to induce differentiation to macrophages. Cells were subsequently harvested, stained with the indicated antibodies, and analyzed by FACS. Figure S4. Vav is required for LPS-induced activation of Erk (JPG, 41.9 KB) - Bone marrow–derived macrophages from WT and Vavnull mice were stimulated with LPS (0.1 µg/mL) for the indicated time points. Activation of Erk MAPK was assayed by probing of immunoblots with antibodies recognizing phosphorylated Erk1/2 (pERK1/2). Protein loading was verified by stripping and reprobing of blots with antibodies against total Erk2. Figure S5. Vav is not required for COX2 induction in response to LPS stimulation (JPG, 56.1 KB) - WT and Vavnull BMDMs were stimulated with LPS (1 or 0.1 µg/mL) and lysed at the indicated time points. Lysates were subsequently analyzed by Western blotting for COX2. The asterisk represents a nonspecific band. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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