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Blood, Vol. 109, Issue 11, 4816-4824, June 1, 2007
Reduced natural killer (NK) function associated with high-risk myelodysplastic syndrome (MDS) and reduced expression of activating NK receptors
Blood Epling-Burnette et al.
109: 4816
Supplemental materials for: Epling-Burnette et al, Vol 109, Issue 11, 4816-4824
Files in this Data Supplement:
- Figure S1. Cell line derived from an MDS patient (MDS1) serves as a target for NK-mediated cytotoxicity (JPG, 59.5 KB)
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(A) To determine whether MDS1 cells elicit direct NK lytic activation, we used normal unactivated PBMCs, normal enriched unactivated NK cells, normal enriched unactivated T cells, and NK cell lines (NK92 and NKL) as effector cells in standard 5-hour 51Cr-release assays. Graphic representations of the percent specific lysis at 50:1, 25:1, 12:1, and 6:1 effector-target (E/T) ratios are shown for normal PBMCs, enriched NK cells, and enriched T cells. Percent specific lysis by NK92 and NKL cells was determined using 10:1, 5:1, 2:1, and 1:1 ratios of effector-target (E/T) cells. The graphs represent the average of triplicate samples; SD is indicated by the error bars. (B) Flow cytometry histograms for MHC class I expression on 721.221, K562, HL-60, and MDS1 cell lines stained with anti–HLA-ABC–FITC antibody. The M1 marker represents positive expression compared with isotype control antibody staining (data not shown).
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